Analyses of nuclear envelope dynamics using a novel model fission yeast

• Project/Area Number: 24770192
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Cell biology
• Research Institution: National Institute of Genetics
• Principal Investigator: AOKI KEITA 国立遺伝学研究所, 系統生物研究センター, 助教 (20620884)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 核膜動態 / 核膜崩壊 / 分裂期制御 / 細胞周期 / S. japonicus / 国際情報交換 / アメリカ

Research Abstract

For the inheritance of genetical information, a nuclear membrane, surrounding DNA, has to be inherited to daughter cells correctly. Higher eukariotic cells like human have 'open' mitosis system accompanying breakdown and regeneration of nuclear membrane. On the other hand, lower eukariotic cells like yeasts have 'closed' mitosis system accompanying shrinkage of nuclear membrane. The significance and mechanism of the difference has not been understood so far.
For our study, a fission yeast S. japonicus, which has 'semi-open' mitosis, was used. Searching genes relating to inheritance of nuclear membrane by genetical screening, it was found that APC/Cyclosome and fatty acid synthase function for the inheritance of nuclear membrane. Because APC/Cyclosome is a key factor in the protein degradation pathway, and fatty acids are important for constructing membrane, we proposed a model that regulation of level of nuclear membrane via protein degradation is necessary for the inheritance of DNA.

Research Products

• [Journal Article] Regulation of nuclear envelope dynamics via APC/C is necessary for the progression of semi-open mitosis in Schizosaccharomyces japonicus (2013)
  • Author(s): 青木敬太、志波優、高田啓、吉川博文、仁木宏典
  • Journal Title: Genes to Cells Volume: 18巻 Pages: 733-752
  • Peer Reviewed
• [Journal Article] Regulation of nuclear envelope dynamics via APC/C is necessary for throgression of semi-open mitosis in Schizosaccharomyces japonicus. (2013)
  • Author(s): Aoki, K., Shiwa, Y., Takada, H., Yoshikawa, H., and Niki, H.
  • Journal Title: Genes to Cells Volume: 18 Issue: 9 Pages: 733-752
  • DOI: 10.1111/gtc.12072
  • Peer Reviewed
• [Journal Article] Genome-wide identification and characterization of replication origins by deep sequencing. (2012)
  • Author(s): Xu, J., Yanagisawa, Y., Tsankov, A.M., Hart, C., Aoki, K., Kommajosyula, N., Steinmann, K.E.,.. Russ, C., Regev, A., Rando, O.J., Nusbaum, C., Niki, H., Milos, P., Weng, Z., & Rhind, N.
  • Journal Title: Genome Biology Volume: 13 Issue: 4 Pages: R27-R27
  • DOI: 10.1186/gb-2012-13-4-r27
  • Peer Reviewed
• [Journal Article] Construction of an insertion marker collection of Sz. japonicus(IMACS) for genetic mapping and a fosmid library covering its genome (2012)
  • Author(s): Furuya, K. Aoki, K. and Niki, H
  • Journal Title: Yeast Volume: 5 Issue: 6 Pages: 241-249
  • DOI: 10.1002/yea.2907
  • Peer Reviewed
• [Presentation] Regulation of nuclear envelope dynamics via APC/C is necessary for the progression of semi-open mitosis in Schizosaccharomyces japonicus (2013)
  • Author(s): 青木敬太、志波優、高田啓、吉川博文、仁木宏典
  • Organizer: The 7th International Fission Yeast Meeting
  • Place of Presentation: UCL, London
• [Presentation] 染色体と核膜構造の維持に必要な因子の解析 (2013)
  • Author(s): 青木敬太、志波優、高田啓、吉川博文、仁木宏典
  • Organizer: 第46回酵母遺伝学フォーラム研究報告会
  • Place of Presentation: 東北学院大学土樋キャンパス押川記念ホール
• [Presentation] 脂肪酸合成とRan経路は、染色体と核膜の構造維持に関わる (2013)
  • Author(s): 青木敬太、志波優、高田啓、吉川博文、仁木宏典
  • Organizer: 第31回染色体ワークショップ、第12回核ダイナミクス研究会
  • Place of Presentation: ホテルおかだ箱根湯本
• [Presentation] 脂肪酸合成とRan経路は、染色体と核膜の構造維持に関わる (2013)
  • Author(s): 青木敬太、志波優、高田啓、吉川博文、仁木宏典
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド
• [Presentation] Regulation of nuclear envelope dynamics via APC/C is necessary for the progression of semi-open mitosis in Schizosaccharomyces japonicus. (2013)
  • Author(s): Keita Aoki, Yuh Shiwa, Hiraku Takada, Hirofumi Yoshikawa, and Hironori Niki.
  • Organizer: The 7th International Fission Yeast Meeting
  • Place of Presentation: UCL, London
• [Presentation] 染色体と核膜構造の維持に必要な因子の解析 (2013)
  • Author(s): 青木敬太、志波優、高田啓、吉川博文、仁木宏典
  • Organizer: 第46回酵母遺伝学フォーラム研究報告会
  • Place of Presentation: 東北学院大学土樋キャンパス 押川記念ホール
• [Presentation] 脂肪酸合成とRan経路は、染色体と核膜の構造維持に関わる (2013)
  • Author(s): 青木敬太、志波優、高田啓、吉川博文、仁木宏典
  • Organizer: 第31回染色体ワークショップ、第12回核ダイナミクス研究会
  • Place of Presentation: ホテルおかだ 箱根湯本
• [Presentation] Oar2を介した核膜動態の制御は、Anaphaseの進行に重要である (2012)
  • Author(s): 青木敬太、志波優、高田啓、吉川博文、仁木宏典
  • Organizer: 第45回酵母遺伝学フォーラム研究報告会
  • Place of Presentation: 京大宇治キャンパス
• [Presentation] Regulation of nuclear envelope dynamics via Oar2 is necessary for the progression of anaphase (2012)
  • Author(s): 青木敬太、志波優、高田啓、吉川博文、仁木宏典
  • Organizer: DYNAMIC ORGANIZATION OF NUCLEAR FUNCTION
  • Place of Presentation: Cold Spring Harbor Laboratory
• [Presentation] Limitation of nuclear envelope dynamics via APC/C is necessary for the progression of semi-open mitosis in Sz. japonicus (2012)
  • Author(s): 青木敬太、志波優、高田啓、吉川博文、仁木宏典
  • Organizer: The 8th 3R Symposium
  • Place of Presentation: 淡路夢舞台
• [Presentation] Regulation for breakage of nuclear envelope via Oar2 is necessary for the progression of anaphase in Sz. japonicus (2012)
  • Author(s): 青木敬太、志波優、高田啓、吉川博文、仁木宏典
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡、マリンメッセ福岡、福岡国際会議場
• [Presentation] Semi-open mitosisの進行に必須な核膜動態制御の解析、第30回染色体ワークショップ (2012)
  • Author(s): 青木敬太、志波優、高田啓、吉川博文、仁木宏典
  • Organizer: 第11回核ダイナミクス研究会
  • Place of Presentation: 淡路夢舞台
• [Presentation] Regulation of nuclear envelope dynamics via Oar2 is necessary for the progression of anaphase (2012)
  • Author(s): 青木敬太、志波優、高田啓、吉川博文、仁木宏典
  • Organizer: DYNAMIC ORGANIZATION OF NUCLEAR FUNCTION
  • Place of Presentation: Cold Spring Harbor Laboratory, USA
• [Presentation] Regulation for breakage of nuclear envelope via Oar2 is necessary for the progression of anaphase in Sz. japonicus (2012)
  • Author(s): 青木敬太、志波優、高田啓、吉川博文、仁木宏典
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: マリンメッセ福岡、福岡国際会議場
• [Presentation] Semi-open mitosisの進行に必須な核膜動態制御の解析 (2012)
  • Author(s): 青木敬太、志波優、高田啓、吉川博文、仁木宏典
  • Organizer: 第30回染色体ワークショップ、第11回核ダイナミクス研究会
  • Place of Presentation: 淡路夢舞台
• [Remarks] (1)タイトル:ジャポニカス酵母菌を材料とした染色体分配の研究
  • URL: http://www.nig.ac.jp/labs/MicroGen/res_act/ourresearch/kobetsu.html
• [Remarks] 仁木研の主要な研究テーマ
  • URL: http://www.nig.ac.jp/labs/MicroGen/res_act/ourresearch/index.html