| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
In this study, I have investigated how a protein kinase IKKepsilon can localize to the distal tip of Drosophila bristle cells. I have demonstrated that IKKepsilon forms a complex with cytoplasmic dynein via an adaptor protein Spn-F. Photobleaching experiments revealed that IKKepsilon/Spn-F complex is selectively retained at the distal tip. Furthermore, I have identified Jvl as the key regulator of the retention of IKKepsilon/Spn-F complex. Thus, IKKepsilon localization is regulated by polarized transport and selective retention. The retention of IKKepsilon/Spn-F complex is in stark contrast with another dynein cargo, Rab11, which displays bidirectional shuttling movement. These results suggest that the distal tip of bristles act as a sorting station for cytoplasmic dynein-dependent cargoes, and suggests that the specific recognition of cargo adaptors plays a key role in cargo sorting. These results unveil the regulatory mechanisms of cytoplasmic dynein-dependent transport.
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