Regulation of motor-cargo interaction in cytoplasmic dynein-dependent intracellular transport
| Project/Area Number |
24770197
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Cell biology
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
OTANI Tetsuhisa 独立行政法人理化学研究所, 多細胞システム形成研究センター, 研究員 (50415105)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 細胞質ダイニン / 細胞内輸送 / 微小管 / IKKε / 細胞極性 / ショウジョウバエ / 細胞伸長 / Spindle-F / 細胞内シグナル伝達 / リン酸化 / 細胞骨格 |
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| Outline of Final Research Achievements |
In this study, I have investigated how a protein kinase IKKepsilon can localize to the distal tip of Drosophila bristle cells. I have demonstrated that IKKepsilon forms a complex with cytoplasmic dynein via an adaptor protein Spn-F. Photobleaching experiments revealed that IKKepsilon/Spn-F complex is selectively retained at the distal tip. Furthermore, I have identified Jvl as the key regulator of the retention of IKKepsilon/Spn-F complex. Thus, IKKepsilon localization is regulated by polarized transport and selective retention. The retention of IKKepsilon/Spn-F complex is in stark contrast with another dynein cargo, Rab11, which displays bidirectional shuttling movement. These results suggest that the distal tip of bristles act as a sorting station for cytoplasmic dynein-dependent cargoes, and suggests that the specific recognition of cargo adaptors plays a key role in cargo sorting. These results unveil the regulatory mechanisms of cytoplasmic dynein-dependent transport.
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Report
(4 results)
Research Products
(17 results)
