Functional analysis of a novel PCP regulator Jitterbug

• Project/Area Number: 24770201
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Developmental biology
• Research Institution: Akita University
• Principal Investigator: AYUKAWA Tomonori 秋田大学, 医学(系)研究科(研究院), その他 (80586165)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 平面内細胞極性 / PCP / 発生生物学 / 遺伝学 / 足場タンパク質

Research Abstract

Most tissues and organs acquire a polarity within the plane of the epithelium, which is orthogonal to the axis of apico-basal polarity. This is called planar cell polarity (PCP). PCP pathway is an evolutionarily conserved mechanism in multicellular organism.
PCP molecules are categorized into two groups by its function. We found that Jbug is categorized into a novel group of PCP regulators (Jbug group). In this study we succeed to identify 9 genes that co-operate with Jbug.

Research Products

• [Journal Article] Rescue of Notch signaling in cells incapable of GDP-L-fucose synthesis by gap junction transfer of GDP-L-fucose in Drosophila (2012)
  • Author(s): Tomonor i Ayukawa, Kenjirou Matsumoto, Hiroyuki O. Ishikawa, Akira Ishio, Tomoko Yamakawa, Naoyuki Aoyama, Takuya Suzuki, Kenji Matsuno
  • Journal Title: Proc Natl Acad Sci U S A Volume: 109(38) Pages: 15318-23
  • Peer Reviewed
• [Journal Article] Metabolism and transportation pathways of GDP-fucose that are required for the O-fucosylation of Notch (2012)
  • Author(s): Tomoko Yamakawa, Tomonor i Ayukawa and Kenji Matsuno
  • Journal Title: Adv Exp Med Biol Volume: 727 Pages: 37-46
  • DOI: 10.1007/978-1-4614-0899-4_3
  • ISBN: 9781461408987, 9781461408994
  • Peer Reviewed
• [Journal Article] Rescue of Notch signaling in cells incapable of GDP-L-fucose synthesis by gap junction transfer of GDP-L-fucose in Drosophila (2012)
  • Author(s): Ayukawa, T., Matsumoto, K., Ishikawa, HO., Ishino, A., Yamakawa, T., Aoyama, N., Suzuki, T., Matsuno. K.
  • Journal Title: Proceeding of the National Academic Sciences Volume: 109(38) Issue: 38 Pages: 15318-15323
  • DOI: 10.1073/pnas.1202369109
  • Peer Reviewed
• [Journal Article] Dachsous-dependent asymmetric localization ofSpiny-legs determines planar cell polarity orientation in Drosophila
  • Author(s): Tomonor i Ayukawa, Masakazu Akiyama, Jennifer L. Mummery-Widmer, Thomas Stoeger, Junko Sasaki, Juergen A. Knoblich, Haruki Senoo, Takehiko Sasaki, Masakazu Yamazaki
  • Journal Title: Cell Reports Volume: (掲載決定)
  • Peer Reviewed
• [Presentation] Prickle and Spiny-legs Determine the Orientation of Cellular Asymmetry Relative to the Dachsous Gradient (2013)
  • Author(s): Tomonori Ayukawa, Masakazu Akiyama, Juergen A. Knoblich, Haruki Senoo, Takehiko Sasaki and Masakazu Yamazaki
  • Organizer: 第46回日本発生生物学会年会
  • Place of Presentation: 松江(ポスター)
• [Presentation] Functional analysis of a novel PCP regulator Jitterbug (2012)
  • Author(s): Tomonor i Ayukawa, Takehiko Sasaki and Masakazu Yamazaki
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡(ショートトーク・ポスター)
• [Presentation] Prickle and Spiny-legs ratio determines the orientation of cellular asymmetry relative to the Dachsous and Four-jointed gradients (2012)
  • Author(s): Tomonor i Ayukawa, Juergen A. Knoblich, Takehiko Sasaki and Masakazu Yamazaki
  • Organizer: Japanese Drosophila Research Conference 10 (JDRC10)
  • Place of Presentation: 東京(口頭発表・ポスター)
• [Presentation] Prickle and Spiny-legs ratio regulates global tissue coordination in planar cell polarity (2012)
  • Author(s): Tomonor i Ayukawa, Juergen A. Knoblich, Takehiko Sasaki and Masakazu Yamazaki
  • Organizer: Joint Meeting of The 45^<th> Annual Meeting of the Japanese Society of Developmental Biologists & The 64^<th> Annual Meeting of the Japan Society for Cell Biology
  • Place of Presentation: obe International Conference Center & The Kobe Chamber of Commerce and Industry,神戸(一般講演・ポスター)
• [Presentation] Prickle and Spiny-legs Determine the Orientation of Cellular Asymmetry Relative to the Dachsous Gradient
  • Author(s): 鮎川友紀
  • Organizer: 第46回日本発生生物学会年会
  • Place of Presentation: くにびきメッセ（松江）
• [Presentation] Functional analysis of a novel PCP regulator Jitterbug
  • Author(s): Tomonori Ayukawa
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場、マリンメッセ福岡（福岡）
• [Presentation] Prickle and Spiny-legs ratio determines the orientation of cellular asymmetry relative to the Dachsous and Four-jointed gradients
  • Author(s): Tomonori Ayukawa
  • Organizer: Japanese Drosophila Research Conference 10 (JDRC10)
  • Place of Presentation: 東京慈恵会医科大学（東京）
• [Presentation] Prickle and Spiny-legs ratio regulates global tissue coordination in planar cell polarity
  • Author(s): Tomonori Ayukawa
  • Organizer: Joint Meeting of The 45th Annual Meeting of the Japanese Society of Developmental Biologists & The 64th Annual Meeting of the Japan Society for Cell Biology
  • Place of Presentation: 神戸国際会議場、神戸商工会議所（神戸）