Novel shRNA library screening to identify key factors that are important in pluripotent stem cells
| Project/Area Number |
24770206
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Developmental biology
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| Research Institution | Kyoto University |
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Principal Investigator |
HOTTA Akitsu 京都大学, iPS細胞研究所, 特定拠点助教 (50578002)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | shRNAライブラリー / トランスポゾンベクター / 発現抑制制御 / 発現抑制 |
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| Outline of Final Research Achievements |
To reveal the molecular mechanisms behind the pluripotency of ES cells and iPS cells, we constructed a piggyBac DNA transposon vector to deliver a genome-wide shRNA library into ES/iPS cells. The generated shRNA library was validated by deep sequencing, and several ten-thousand shRNAs were successfully cloned into our piggyBac vector. Our piggyBac shRNA library is a versatile tool to dissect the molecular mechanisms in pluripotent stem cells and other cell types, without the use of viral vectors.
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Report
(4 results)
Research Products
(2 results)
