| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Outline of Final Research Achievements |
In this study, we aimed at the understanding of mammalian fertilization, especially sperm-egg fusion and egg activation by visualizing these phenomena using gene manipulated animal and live imaging technique. First, it was succeeded to visualize the moment of sperm-egg fusion in live using fluorescence-tagged IZUMO1 and CD9, and the significance of endocytotic activity of egg was strongly implicated. And, by the generation of gene-manipulated mouse line which lacks a newly reported sperm-born egg activation factor, it was proven that the factor doesn’t play any essential role in mouse egg activation.
Additionally, we succeeded to develop a novel gene-editing method using CRISPR/Cas9 system for rapid generation of gene deleted or point mutant mice, which enabled us to generate the multiple lines of mutant mice for essential factor(s) in sperm-egg fusion or egg activation.
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