Robustness mechanisms for gene expression though the paralogous genes
| Project/Area Number |
24770214
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Developmental biology
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| Research Institution | Yamagata University |
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Principal Investigator |
OCHI Haruki 山形大学, 医学部, 助教 (00505787)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | パラログ / 発生制御遺伝子 / Pax2 / Pax5 / ナメクジウオ / ツメガエル / トランスジェニックシステム / 浸透圧ストレス / 遺伝子発現制御 / 遺伝子発現調節 / 転写因子 / 器官形成 / ゲノム進化 / 遺伝子発現 / シス調節配列 / パラログ遺伝子 |
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| Outline of Final Research Achievements |
Paralogus genes often have redundant functions, while little is known about the evolution of their regulatory interactions. It has been reported that, while pax2 and ancestral-type ortholog pax2/5/8 show constitutive expression in the pronephros and its orthologous tissue in amphioxus respectively, pax5 shows no expression in the pronephros. We found that pax5 gets activated in the pronephros, by either the down-regulation of pax2, or by hypertonic osmotic stress. We identified a pronephric enhancer of pax2, and revealed that its paralogous counterpart in pax5 is involved in both the functional compensation of pax2 and the osmotic stress response. Their ancestral-type enhancer in pax2/5/8 was also active in the normal pronephros but got further activated by the hypertonic osmotic stress. These results suggest that the mechanisms for the functional compensation of pax2 by pax5 evolved from the osmotic stress response mechanism of their ancestral gene.
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Report
(4 results)
Research Products
(44 results)
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[Presentation] Evolution of a tissue-specific silencer underlies diversification of paralogous genes2013
Author(s)
Ochi, H., Tamai, T., Nagano, H., Kawaguchi, A., Sudou, N. and Ogino, H.
Organizer
The 17th International Congress of Developmental Biology
Place of Presentation
Mexico, Cancun, Cancun Center, Conventions & Exhibitions
Related Report
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[Presentation] Role of Six1 in evolution of vertebrate primary sensory system2013
Author(s)
Kawakami, K., Yajima, H., Suzuki, M., Ochi, H., Ikeda, K., Sato, S., Ogino, H., Ueno, N.
Organizer
46th Annual Meeting for the Japanese Society of Developmental Biologists
Place of Presentation
島根県島根市くにびきメッセ
Related Report
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[Presentation] Paralogous enhancers: a crossover point between developmental robustness and Stress response2012
Author(s)
Ochi, H., Tamai, T., Nagano, H., Kawaguchi, A., Sudou, N. and Ogino, H
Organizer
The 14th Xenopus international conference
Place of Presentation
The Giens Peninsula (Toulon, France)
Related Report
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[Presentation] Evolution of a tissue-specific silencer underlies divergence in the expression of paralogues2012
Author(s)
Ogino, H., Ochi, H., Tamai, T., Nagano, H., Kawaguchi, A. and Sudou, N.
Organizer
The 14th Xenopus international conference
Place of Presentation
The Giens Peninsula (Toulon, France)
Related Report
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[Presentation] Key regulator for development and evolutionary switch form Rohon-Beard cells to dorsal root ganglia2012
Author(s)
Yajima, H., Suzuki, M., Ochi, H., Ikeda, K., Sato, S., Ogino, H., Ueno, N. and Kawakami, K
Organizer
ISDB 71th Annual Meeting
Place of Presentation
Hilton Bonaventure Hotel (Montreal, Canada)
Related Report
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