| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Research Abstract |
In the present study, using a testis tissue culture method, we challenged to treat a spermatogenic defect mutant without any genetic manipulations. The Sl/Sld mouse has only a few primitive spermatogonia as germ cells in the testis, lacking any sign of spermatogenesis, due to mutations of the c-Kit ligand gene (Kitl) which cause the loss of membrane-bound-type KITL from the surface of Sertoli cells. We succeeded in inducing the complete spermatogenesis of Sl/Sld mutant mice by culturing their testis tissues in culture media containing KITL and colony stimulating factor-1 (CSF-1).To elucidate the mechanism of CSF-1's effect on spermatogenesis, we performed transplantation experiment of spermatogonial stem cells derived from CSF-1 receptor knock out mouse.
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