| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Outline of Final Research Achievements |
Spatial distribution of morphogens and polarity proteins is scaled to cell and embryonic size. To reveal molecular and physical basis of the scaling, we measured intracellular protein dynamics using single-molecule detection technologies. By incorporating the parameters to describe the protein dynamics in vivo into a mathematical model, we succeeded in reproducing asymmetric localization of polarity protein PAR-2. Based on the in vivo measurements and theoretical analyses, we demonstrated that the asymmetric PAR-2 localization is maintained by a rapid-local exchange of PAR-2 between the cortex and cytoplasm. We will investigate how the rapid-local exchange contributes to the scaling.
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