Scaling mechanism revealed by single-molecule detection technologies and mathematical modeling
Project/Area Number |
24770221
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Developmental biology
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Research Institution | The Institute of Physical and Chemical Research |
Principal Investigator |
ARATA Yukinobu 独立行政法人理化学研究所, 佐甲細胞情報研究室, 研究員 (40360482)
|
Project Period (FY) |
2012-04-01 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
|
Keywords | 細胞極性 / PAR/aPKC / 1分子イメージング / 定量計測 / 数理モデル / 1分子イメージング / PAR / 1分子計測 / 数理モデル化 / 1分子計測 / 精密計測 |
Outline of Final Research Achievements |
Spatial distribution of morphogens and polarity proteins is scaled to cell and embryonic size. To reveal molecular and physical basis of the scaling, we measured intracellular protein dynamics using single-molecule detection technologies. By incorporating the parameters to describe the protein dynamics in vivo into a mathematical model, we succeeded in reproducing asymmetric localization of polarity protein PAR-2. Based on the in vivo measurements and theoretical analyses, we demonstrated that the asymmetric PAR-2 localization is maintained by a rapid-local exchange of PAR-2 between the cortex and cytoplasm. We will investigate how the rapid-local exchange contributes to the scaling.
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Report
(4 results)
Research Products
(15 results)