Genomic and chromosomal evolution of vertebrates inferred from comparative gene mapping

• Project/Area Number: 24770226
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Evolutionary biology
• Research Institution: Nagoya University
• Principal Investigator: UNO Yoshinobu 名古屋大学, 生命農学研究科, 研究員 (60609717)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2013: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: FISH / 核型進化 / マイクロ染色体 / 脊椎動物 / 染色体地図 / 両生類 / Xenopus / 異質四倍体 / ゲノム重複 / 軟骨魚類 / 肉鰭類

Research Abstract

To deduce the process of genomic and chromosomal evolution in vertebrates, I constructed a chromosome map of Xenopus (Silurana) tropicalis using FISH mapping. The homologous segments corresponding to chicken microchromosomes have been highly conserved in X. tropicalis without microchromosomes, suggesting that the ancestral tetrapods probably had genetic linkage groups each of which corresponded to chicken microchromosomes. Next, I performed FISH mapping for an allotetraploid species, X. laevis, and constructed a comparative chromosome map between these two Xenopus species. This result indicated that the loss of duplicated genes and interchromosomal rearrangements occurred much less frequently in X. laevis allotetraploidization.

Research Products

• [Journal Article] Homoeologous chromosomes of Xenopus laevis are highly conserved after whole genome duplication (2013)
  • Author(s): Uno Y, Nishida C, Takagi C, Ueno N, Matsuda Y
  • Journal Title: Heredity Volume: 111 Issue: 5 Pages: 430-436
  • DOI: 10.1038/hdy.2013.65
  • Peer Reviewed
• [Journal Article] Inference of the protokaryotypes of amniotes and tetrapods and the evolutionary processes of microchromosomes from comparative gene mapping (2012)
  • Author(s): Uno Y, Nishida C, Tarui H, Ishishita S, Takagi C, Nishimura O, Ishijima J, Ota H, Kosaka A, Matsubara K, Murakami Y, Kuratani S, Ueno N, Agata K, Matsuda Y
  • Journal Title: PLoS One Volume: 7 Issue: 12 Pages: e53027-e53027
  • DOI: 10.1371/journal.pone.0053027
  • Peer Reviewed
• [Presentation] Molecular cytogenetic studies on the process of genomic and chromosomal evolution in X. laevis after WGD and the origin and evolution of sex chromosomes in anuran species (2014)
  • Author(s): Uno Y, Nishida C, Takagi C, Igawa T, Ueno N, Sumida M and Matsuda Y.
  • Organizer: International symposium Frontiers in Amphibian Biology : Endangered Species Conservation and Genome Editing
  • Place of Presentation: Hiroshima University(東広島市)
• [Presentation] Molecular cytogenetic studies on the process of genomic and chromosomal evolution in X. laevis after WGD and the origin and evolution of sex chromosomes in anuran species (2014)
  • Author(s): Yoshinobu Uno, Chizuko Nishida, Chiyo Takagi, Takeshi Igawa, Naoto Ueno, Masayuki Sumida, Yoichi Matsuda
  • Organizer: International symposium Frontiers in Amphibian Biology: Endangered Species Conservation and Genome Editing
  • Place of Presentation: 広島大学（東広島）
• [Presentation] アフリカツメガエルのゲノム倍数化過程に生じた染色体再配列と無尾両生類における性染色体の起源とその進化に関する分子細胞遺伝学的研究 (2013)
  • Author(s): 宇野好宣、西田千鶴子、高木知世、井川武、上野直人、住田正幸、松田洋一
  • Organizer: 日本遺伝学会第85回大会
  • Place of Presentation: 慶應義塾大学(横浜市)
• [Presentation] Molecular cytogenetic studies on the process of genomic and chromosomal evolution in X. laevis after WGD and the origin and evolution of sex chromosomes in anuran species (2013)
  • Author(s): Uno Y, Nishida C, Takagi C, Igawa T, Ueno N, Sumida M and Matsuda Y.
  • Organizer: The 19th International Chromosome Conference
  • Place of Presentation: University of Bologna (イタリア・ボローニャ)
• [Presentation] アフリカツメガエルのゲノム倍数化過程に生じた染色体再配列と無尾両生類における性染色体の起源とその進化に関する分子細胞遺伝学的研究 (2013)
  • Author(s): 宇野好宣、西田千鶴子、高木知世、井川武、上野直人、住田正幸、松田洋一
  • Organizer: 日本遺伝学会 第85回大会
  • Place of Presentation: 慶應義塾大学（横浜）
• [Presentation] Molecular cytogenetic studies on the process of genomic and chromosomal evolution in X. laevis after WGD and the origin and evolution of sex chromosomes in anuran species (2013)
  • Author(s): Yoshinobu Uno, Chizuko Nishida, Chiyo Takagi, Takeshi Igawa, Naoto Ueno, Masayuki Sumida, Yoichi Matsuda
  • Organizer: The 19th International Chromosome Conference
  • Place of Presentation: University of Bologna（イタリア・ボローニャ）