| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
Molecular analysis of the genes specifically expressed in brood pouch and morphological analysis of the pouch were performed in the present study to understand how and why the brood pouch was formed during the evolution of seahorses. Expression levels of the genes in brood pouch were compared between brooding males and non-brooding males. As the results, 100 genes were found as up-regulated genes in brooding males. Their full-length sequences were determined, and their localizations were analyzed by in situ hybridization or by immunohistochemistry. These genes can be used for marker for formation of the brood pouch, by analyzing localization of the genes during the development of the brood pouch. The present study provides back ground to study evolutionary pathway of the formation of brood pouch in seahorses.
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