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Mechanism of formation of brood pouch during the evolution of seahorses

Research Project

Project/Area Number 24770231
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Evolutionary biology
Research InstitutionSophia University

Principal Investigator

KAWAGUCHI Mari  上智大学, 理工学部, 助教 (00612095)

Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywordsタツノオトシゴ / 育児嚢 / パトリスタシン / C型レクチン / コラーゲン / 子育て / 分子進化 / アスタシンファミリー / レクチン
Outline of Final Research Achievements

Molecular analysis of the genes specifically expressed in brood pouch and morphological analysis of the pouch were performed in the present study to understand how and why the brood pouch was formed during the evolution of seahorses. Expression levels of the genes in brood pouch were compared between brooding males and non-brooding males. As the results, 100 genes were found as up-regulated genes in brooding males. Their full-length sequences were determined, and their localizations were analyzed by in situ hybridization or by immunohistochemistry. These genes can be used for marker for formation of the brood pouch, by analyzing localization of the genes during the development of the brood pouch. The present study provides back ground to study evolutionary pathway of the formation of brood pouch in seahorses.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (25 results)

All 2014 2013 2012

All Journal Article (6 results) (of which Peer Reviewed: 6 results,  Acknowledgement Compliant: 2 results,  Open Access: 1 results) Presentation (17 results) Book (2 results)

  • [Journal Article] Molecular events in adaptive evolution of the hatching strategy of ovoviviparous fishes.2014

    • Author(s)
      Mari Kawaguchi, Kenji Tomita, Kaori Sano, Toyoji Kaneko.
    • Journal Title

      Journal of Experimental Zoology Part B

      Volume: 324 Issue: 1 Pages: 41-50

    • DOI

      10.1002/jez.b.22601

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Comparison of hatching mode in pelagic and demersal eggs of two closely related species in the order Pleuronectiformes.2014

    • Author(s)
      Mari Kawaguchi, Kaori Sano, Norio Yoshizaki, Daisuke Shimizu, Yuichiro Fujinami, Tsutomu Noda, and Shigeki Yasumasu
    • Journal Title

      Zoological Science

      Volume: 31 Issue: 11 Pages: 709-715

    • DOI

      10.2108/zs140018

    • NAID

      40020278661

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Molecular co-evolution of a protease and its substrate elucidated by analysis of the activity of predicted ancestral hatching enzyme.2013

    • Author(s)
      Kawaguchi, M., K. Inoue, I. Iuchi, M. Nishida, S. Yasumasu
    • Journal Title

      BMC Evolutionary Biology

      Volume: 13 Issue: 1 Pages: 231-231

    • DOI

      10.1186/1471-2148-13-231

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Sub-functionalization of duplicated genes in the evolution of nine-spined stickleback hatching enzyme2013

    • Author(s)
      Mari Kawaguchi
    • Journal Title

      Journal of Experimental Zoology Part B: Molecular and Developmental Evolution

      Volume: 320 Issue: 3 Pages: 140-150

    • DOI

      10.1002/jez.b.22490

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Adaptive evolution of fish hatching enzyme: one amino acid substitution results in differential salt dependency of the enzyme.2013

    • Author(s)
      Kawaguchi, M., S. Yasumasu, A. Shimizu, N. Kudo, K. Sano, I. Iuchi, M. Nishida
    • Journal Title

      The Journal of Experimental Biology

      Volume: 216 Pages: 1609-1615

    • DOI

      10.1242/jeb.069716

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Inferring the evolution of teleostean zp genes based on their sites of expression2013

    • Author(s)
      Kaori Sano, Mari Kawaguchi, Satoshi Watanabe, Yoshitomo Nagakura, Takashi Hiraki, Shigeki Yasumasu
    • Journal Title

      Journal of Experimental Zoology Part B: Molecular and Developmental Evolution

      Volume: 未定

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] タツノオトシゴの育児嚢特異的なC型レクチンのクローン化と発現局在2014

    • Author(s)
      大窪遼平・高田健介・川口眞理
    • Organizer
      第85回日本動物学会
    • Place of Presentation
      仙台
    • Year and Date
      2014-09-11 – 2014-09-13
    • Related Report
      2014 Annual Research Report
  • [Presentation] タツノオトシゴの育児嚢特異的アスタシンプロテアーゼファミリー遺伝子2014

    • Author(s)
      川口眞理・中野悠子・川原玲香・今福愛子
    • Organizer
      第16回日本進化学会
    • Place of Presentation
      大阪
    • Year and Date
      2014-08-21 – 2014-08-24
    • Related Report
      2014 Annual Research Report
  • [Presentation] patristacin遺伝子の進化2014

    • Author(s)
      中野悠子・今福愛子・川口眞理
    • Organizer
      第66回日本動物学会関東支部大会
    • Place of Presentation
      千葉
    • Related Report
      2013 Research-status Report
  • [Presentation] メダカ属の孵化酵素HCEの至適塩濃度2014

    • Author(s)
      川口眞理・井上広滋・安増茂樹
    • Organizer
      第66回日本動物学会関東支部大会
    • Place of Presentation
      千葉
    • Related Report
      2013 Research-status Report
  • [Presentation] メダカ孵化酵素HCEの2つのアイソザイムの至適塩濃度2013

    • Author(s)
      川口眞理・井上広滋・安増茂樹
    • Organizer
      第84回日本動物学会
    • Place of Presentation
      岡山
    • Related Report
      2013 Research-status Report
  • [Presentation] ニシンの卵膜の2層構造~卵巣由来と肝臓由来の卵膜~2013

    • Author(s)
      佐野香織・川口眞理・富田憲司・安増茂樹
    • Organizer
      第84回日本動物学会
    • Place of Presentation
      岡山
    • Related Report
      2013 Research-status Report
  • [Presentation] アカハライモリ胃部域アスパラギン酸プロテアーゼのクローニング2013

    • Author(s)
      長澤竜樹・佐野香織・川口眞理・小林健一郎・安増茂樹・井口智文
    • Organizer
      第84回日本動物学会
    • Place of Presentation
      岡山
    • Related Report
      2013 Research-status Report
  • [Presentation] 卵胎生魚の孵化酵素遺伝子2013

    • Author(s)
      川口眞理・安増茂樹
    • Organizer
      第15回日本進化学会
    • Place of Presentation
      つくば
    • Related Report
      2013 Research-status Report
  • [Presentation] 魚類の2つの進化系統において異なった運命をたどった孵化酵素の重複遺伝子2013

    • Author(s)
      佐野香織・川口眞理・渡部諭史・安増茂樹
    • Organizer
      第15回日本進化学会
    • Place of Presentation
      つくば
    • Related Report
      2013 Research-status Report
  • [Presentation] タツノオトシゴのアスタシンファミリープロテアーゼ遺伝子の探査2013

    • Author(s)
      今福愛子・川口眞理
    • Organizer
      第65回日本動物学会関東支部大会
    • Place of Presentation
      東京
    • Related Report
      2012 Research-status Report
  • [Presentation] タツノオトシゴcollectin placenta遺伝子のクローン化と組換えタンパク質の作成2013

    • Author(s)
      木浦知香・川口眞理
    • Organizer
      第65回日本動物学会関東支部大会
    • Place of Presentation
      東京
    • Related Report
      2012 Research-status Report
  • [Presentation] タツノオトシゴの育児嚢で発現している遺伝子の網羅的解析2013

    • Author(s)
      宮地央・川原玲香・河野友宏・川口眞理
    • Organizer
      第65回日本動物学会関東支部大会
    • Place of Presentation
      東京
    • Related Report
      2012 Research-status Report
  • [Presentation] Sub-functionalization of duplicated genes during evolution.2012

    • Author(s)
      Mari Kawaguchi, Hiroshi Takahashi, and Shigeki Yasumasu
    • Organizer
      Stickleback 2012 7th International Conference on Stickleback Behavior and Evolution
    • Place of Presentation
      Seattle, Washington, USA
    • Related Report
      2012 Research-status Report
  • [Presentation] Inferring the evolution of teleostean zp genes based on the site of expression.2012

    • Author(s)
      Kaori Sano, Mari Kawaguchi, and Shigeki Yasumasu
    • Organizer
      International Symposium on the Mechanisms of Sexual Reproduction in Animals and Plants. Joint Meeting of the 2nd Allo-authenticating Meetinga and the 5th Egg-Coat Meeting (MCBEEC)
    • Place of Presentation
      Nagoya, Japan
    • Related Report
      2012 Research-status Report
  • [Presentation] トゲウオ類における孵化酵素の遺伝子重複による機能分化2012

    • Author(s)
      川口眞理・高橋洋・西田睦・安増茂樹
    • Organizer
      第83回日本動物学会
    • Place of Presentation
      大阪
    • Related Report
      2012 Research-status Report
  • [Presentation] トミヨ孵化酵素LCEの卵膜分解における機能分化2012

    • Author(s)
      川口眞理・高橋洋・西田睦・安増茂樹
    • Organizer
      第14回日本進化学会
    • Place of Presentation
      東京
    • Related Report
      2012 Research-status Report
  • [Presentation] 魚類の卵膜構成タンパク質とその遺伝子の進化2012

    • Author(s)
      佐野香織・川口眞理・安増茂樹
    • Organizer
      第14回日本進化学会
    • Place of Presentation
      東京
    • Related Report
      2012 Research-status Report
  • [Book] Oxford: Academic Press2013

    • Author(s)
      Shigeki Yasumasu and Mari Kawaguchi
    • Total Pages
      5
    • Publisher
      Choriolysin H. In Neil D. Rawlings and Guy S. Salvesen, editors: Handbook of Proteolytic Enzymes
    • Related Report
      2012 Research-status Report
  • [Book] Oxford: Academic Press2013

    • Author(s)
      Shigeki Yasumasu and Mari Kawaguchi
    • Total Pages
      4
    • Publisher
      Choriolysin L. In Neil D. Rawlings and Guy S. Salvesen, editors: Handbook of Proteolytic Enzymes
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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