Mechanism of formation of brood pouch during the evolution of seahorses

• Project/Area Number: 24770231
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Evolutionary biology
• Research Institution: Sophia University
• Principal Investigator: KAWAGUCHI Mari 上智大学, 理工学部, 助教 (00612095)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: タツノオトシゴ / 育児嚢 / パトリスタシン / C型レクチン / コラーゲン / 子育て / 分子進化 / アスタシンファミリー / レクチン

Outline of Final Research Achievements

Molecular analysis of the genes specifically expressed in brood pouch and morphological analysis of the pouch were performed in the present study to understand how and why the brood pouch was formed during the evolution of seahorses. Expression levels of the genes in brood pouch were compared between brooding males and non-brooding males. As the results, 100 genes were found as up-regulated genes in brooding males. Their full-length sequences were determined, and their localizations were analyzed by in situ hybridization or by immunohistochemistry. These genes can be used for marker for formation of the brood pouch, by analyzing localization of the genes during the development of the brood pouch. The present study provides back ground to study evolutionary pathway of the formation of brood pouch in seahorses.

Research Products

• [Journal Article] Molecular events in adaptive evolution of the hatching strategy of ovoviviparous fishes. (2014)
  • Author(s): Mari Kawaguchi, Kenji Tomita, Kaori Sano, Toyoji Kaneko.
  • Journal Title: Journal of Experimental Zoology Part B Volume: 324 Issue: 1 Pages: 41-50
  • DOI: 10.1002/jez.b.22601
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Comparison of hatching mode in pelagic and demersal eggs of two closely related species in the order Pleuronectiformes. (2014)
  • Author(s): Mari Kawaguchi, Kaori Sano, Norio Yoshizaki, Daisuke Shimizu, Yuichiro Fujinami, Tsutomu Noda, and Shigeki Yasumasu
  • Journal Title: Zoological Science Volume: 31 Issue: 11 Pages: 709-715
  • DOI: 10.2108/zs140018
  • NAID: 40020278661
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Molecular co-evolution of a protease and its substrate elucidated by analysis of the activity of predicted ancestral hatching enzyme. (2013)
  • Author(s): Kawaguchi, M., K. Inoue, I. Iuchi, M. Nishida, S. Yasumasu
  • Journal Title: BMC Evolutionary Biology Volume: 13 Issue: 1 Pages: 231-231
  • DOI: 10.1186/1471-2148-13-231
  • Peer Reviewed
• [Journal Article] Sub-functionalization of duplicated genes in the evolution of nine-spined stickleback hatching enzyme (2013)
  • Author(s): Mari Kawaguchi
  • Journal Title: Journal of Experimental Zoology Part B: Molecular and Developmental Evolution Volume: 320 Issue: 3 Pages: 140-150
  • DOI: 10.1002/jez.b.22490
  • Peer Reviewed
• [Journal Article] Adaptive evolution of fish hatching enzyme: one amino acid substitution results in differential salt dependency of the enzyme. (2013)
  • Author(s): Kawaguchi, M., S. Yasumasu, A. Shimizu, N. Kudo, K. Sano, I. Iuchi, M. Nishida
  • Journal Title: The Journal of Experimental Biology Volume: 216 Pages: 1609-1615
  • DOI: 10.1242/jeb.069716
  • Peer Reviewed
• [Journal Article] Inferring the evolution of teleostean zp genes based on their sites of expression (2013)
  • Author(s): Kaori Sano, Mari Kawaguchi, Satoshi Watanabe, Yoshitomo Nagakura, Takashi Hiraki, Shigeki Yasumasu
  • Journal Title: Journal of Experimental Zoology Part B: Molecular and Developmental Evolution Volume: 未定
  • Peer Reviewed
• [Presentation] タツノオトシゴの育児嚢特異的なC型レクチンのクローン化と発現局在 (2014)
  • Author(s): 大窪遼平・高田健介・川口眞理
  • Organizer: 第85回日本動物学会
  • Place of Presentation: 仙台
  • Year and Date: 2014-09-11 – 2014-09-13
• [Presentation] タツノオトシゴの育児嚢特異的アスタシンプロテアーゼファミリー遺伝子 (2014)
  • Author(s): 川口眞理・中野悠子・川原玲香・今福愛子
  • Organizer: 第16回日本進化学会
  • Place of Presentation: 大阪
  • Year and Date: 2014-08-21 – 2014-08-24
• [Presentation] patristacin遺伝子の進化 (2014)
  • Author(s): 中野悠子・今福愛子・川口眞理
  • Organizer: 第66回日本動物学会関東支部大会
  • Place of Presentation: 千葉
• [Presentation] メダカ属の孵化酵素HCEの至適塩濃度 (2014)
  • Author(s): 川口眞理・井上広滋・安増茂樹
  • Organizer: 第66回日本動物学会関東支部大会
  • Place of Presentation: 千葉
• [Presentation] メダカ孵化酵素HCEの2つのアイソザイムの至適塩濃度 (2013)
  • Author(s): 川口眞理・井上広滋・安増茂樹
  • Organizer: 第84回日本動物学会
  • Place of Presentation: 岡山
• [Presentation] ニシンの卵膜の2層構造～卵巣由来と肝臓由来の卵膜～ (2013)
  • Author(s): 佐野香織・川口眞理・富田憲司・安増茂樹
  • Organizer: 第84回日本動物学会
  • Place of Presentation: 岡山
• [Presentation] アカハライモリ胃部域アスパラギン酸プロテアーゼのクローニング (2013)
  • Author(s): 長澤竜樹・佐野香織・川口眞理・小林健一郎・安増茂樹・井口智文
  • Organizer: 第84回日本動物学会
  • Place of Presentation: 岡山
• [Presentation] 卵胎生魚の孵化酵素遺伝子 (2013)
  • Author(s): 川口眞理・安増茂樹
  • Organizer: 第15回日本進化学会
  • Place of Presentation: つくば
• [Presentation] 魚類の２つの進化系統において異なった運命をたどった孵化酵素の重複遺伝子 (2013)
  • Author(s): 佐野香織・川口眞理・渡部諭史・安増茂樹
  • Organizer: 第15回日本進化学会
  • Place of Presentation: つくば
• [Presentation] タツノオトシゴのアスタシンファミリープロテアーゼ遺伝子の探査 (2013)
  • Author(s): 今福愛子・川口眞理
  • Organizer: 第65回日本動物学会関東支部大会
  • Place of Presentation: 東京
• [Presentation] タツノオトシゴcollectin placenta遺伝子のクローン化と組換えタンパク質の作成 (2013)
  • Author(s): 木浦知香・川口眞理
  • Organizer: 第65回日本動物学会関東支部大会
  • Place of Presentation: 東京
• [Presentation] タツノオトシゴの育児嚢で発現している遺伝子の網羅的解析 (2013)
  • Author(s): 宮地央・川原玲香・河野友宏・川口眞理
  • Organizer: 第65回日本動物学会関東支部大会
  • Place of Presentation: 東京
• [Presentation] Sub-functionalization of duplicated genes during evolution. (2012)
  • Author(s): Mari Kawaguchi, Hiroshi Takahashi, and Shigeki Yasumasu
  • Organizer: Stickleback 2012 7th International Conference on Stickleback Behavior and Evolution
  • Place of Presentation: Seattle, Washington, USA
• [Presentation] Inferring the evolution of teleostean zp genes based on the site of expression. (2012)
  • Author(s): Kaori Sano, Mari Kawaguchi, and Shigeki Yasumasu
  • Organizer: International Symposium on the Mechanisms of Sexual Reproduction in Animals and Plants. Joint Meeting of the 2nd Allo-authenticating Meetinga and the 5th Egg-Coat Meeting (MCBEEC)
  • Place of Presentation: Nagoya, Japan
• [Presentation] トゲウオ類における孵化酵素の遺伝子重複による機能分化 (2012)
  • Author(s): 川口眞理・高橋洋・西田睦・安増茂樹
  • Organizer: 第83回日本動物学会
  • Place of Presentation: 大阪
• [Presentation] トミヨ孵化酵素LCEの卵膜分解における機能分化 (2012)
  • Author(s): 川口眞理・高橋洋・西田睦・安増茂樹
  • Organizer: 第14回日本進化学会
  • Place of Presentation: 東京
• [Presentation] 魚類の卵膜構成タンパク質とその遺伝子の進化 (2012)
  • Author(s): 佐野香織・川口眞理・安増茂樹
  • Organizer: 第14回日本進化学会
  • Place of Presentation: 東京
• [Book] Oxford: Academic Press (2013)
  • Author(s): Shigeki Yasumasu and Mari Kawaguchi
  • Total Pages: 5
  • Publisher: Choriolysin H. In Neil D. Rawlings and Guy S. Salvesen, editors: Handbook of Proteolytic Enzymes
• [Book] Oxford: Academic Press (2013)
  • Author(s): Shigeki Yasumasu and Mari Kawaguchi
  • Total Pages: 4
  • Publisher: Choriolysin L. In Neil D. Rawlings and Guy S. Salvesen, editors: Handbook of Proteolytic Enzymes