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Elucidation of post-transcriptional regulation involved in plant traits and epigenome analysis in Brassica

Research Project

Project/Area Number 24780002
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Breeding science
Research InstitutionKobe University (2013)
Niigata University (2012)

Principal Investigator

FUJIMOTO Ryo  神戸大学, (連合)農学研究科(研究院), 准教授 (60620375)

Research Collaborator KAWANABE Takahiro  (株)渡辺採種場, 研究員
KAJI Makoto  (株)渡辺採種場, 研究部, 部長 (60545010)
OKAZAKI Keiichi  新潟大学, 大学院自然科学研究科, 教授 (20270936)
Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywordsエピゲノム / アブラナ科
Research Abstract

The aim of this research is to elucidate of the epigenetic status of Brassica rapa at the whole genome level. We examined the DNA methylation and histone modification status at the whole genome level. Next, we assessed the change of epigenetic modification after vernalization or infection by pathogens. We found accumulation of H3K27me3 level at the FLC locus and repression of its expression after vernalization.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (20 results)

All 2014 2013 2012

All Journal Article (5 results) (of which Peer Reviewed: 3 results) Presentation (13 results) (of which Invited: 1 results) Book (2 results)

  • [Journal Article] Identification of candidate genes for fusarium yellows resistance in Chinese cabbage by differential expression analysis.2014

    • Author(s)
      Shimizu M, Fujimoto R, Ying H, Pu ZJ, Ebe Y, Kawanabe T, Saeki N, Taylor JM, Kaji M, Dennis ES, Okazaki K
    • Journal Title

      Plant Molecular Biology

      Volume: (印刷中) Issue: 3 Pages: 889-900

    • DOI

      10.1007/s11103-014-0182-0

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Transcriptional regulation of key gene of vernalization, FLOWERING LOCUS C2014

    • Author(s)
      Tomaru Y, Osabe K, Okazaki K, Fujimoto R.
    • Journal Title

      Bull Facul Agric Niigata Univ

      Volume: 66 Pages: 105-110

    • NAID

      120006746771

    • Related Report
      2013 Final Research Report
  • [Journal Article] Transcriptional regulation of key gene of vernalization, FLOWERING LOCUS C2014

    • Author(s)
      Yuki Tomaru
    • Journal Title

      Bulletin of the Faculty of Agriculture, Niigata University

      Volume: 66 Pages: 105-110

    • Related Report
      2013 Annual Research Report
  • [Journal Article] Accumulation of quantitative trait loci conferring broad- spectrum clubroot resistance in Brassica oleracea2013

    • Author(s)
      H. TOMITA, M. SHIMIZU, MA U. DOULLAH, R. FUJIMOTO, K. OKAZAKI
    • Journal Title

      Mol Breeding

      Volume: 32 Issue: 4 Pages: 889-900

    • DOI

      10.1007/s11032-013-9918-9

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Molecular mechanisms of epigenetic variation in plants2012

    • Author(s)
      Ryo Fujimoto, Taku Sasaki, Ryo Ishikawa, Kenji Osabe, Takahiro Kawanabe, Elizabeth Dennis
    • Journal Title

      Int. J. Mol. Sci.

      Volume: 13 Issue: 8 Pages: 9900-9922

    • DOI

      10.3390/ijms13089900

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Presentation] Differential expression解析を用いたハクサイ萎黄病抵抗性候補遺伝子の同定2014

    • Author(s)
      清水元樹、江部裕介、川辺隆大、佐伯なつみ、加治誠、岡崎桂一、藤本龍
    • Organizer
      園芸学会平成26年度春季大会
    • Place of Presentation
      筑波大学
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] Brassica oleracea品種における萎黄病抵抗性遺伝子FocBo1連鎖DNAマーカーと抵抗性との関連性2013

    • Author(s)
      清水元樹、川辺隆大、Pu Zi-jing、藤本龍、岡崎桂一
    • Organizer
      日本育種学会第125回講演会
    • Place of Presentation
      鹿児島大学
    • Year and Date
      2013-10-13
    • Related Report
      2013 Final Research Report
  • [Presentation] Brassica oleracea染色体添加型B. rapaを用いた緑植物体春化の遺伝解析2013

    • Author(s)
      戸丸祐貴、中村友理、清水元樹、藤本龍、岡崎桂一
    • Organizer
      日本育種学会第124回講演会
    • Place of Presentation
      鹿児島大学
    • Year and Date
      2013-10-13
    • Related Report
      2013 Final Research Report
  • [Presentation] アブラナ科植物の種内、種間雑種に見られる非相加的遺伝子発現2013

    • Author(s)
      藤本龍
    • Organizer
      日本育種学会第123回講演会グループ研究集会
    • Place of Presentation
      東京農業大学
    • Year and Date
      2013-03-28
    • Related Report
      2013 Final Research Report
  • [Presentation] Brassica oleracea染色体添加型 B. rapa を用いた緑植物体春化の遺伝解析2013

    • Author(s)
      戸丸祐貴
    • Organizer
      日本育種学会第124回講演会
    • Place of Presentation
      鹿児島大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] Brassica oleracea品種における萎黄病抵抗性遺伝子FocBo1連鎖DNA マーカーと抵抗性との関連性2013

    • Author(s)
      清水元樹
    • Organizer
      日本育種学会第124回講演会
    • Place of Presentation
      鹿児島大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] アブラナ科植物の種内、種間雑種に見られる非相加的遺伝子発現2013

    • Author(s)
      藤本龍
    • Organizer
      日本育種学会
    • Place of Presentation
      東京農業大学
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] RNA-sequence法を用いたハクサイ品種間(S11×R09)特異的なNBS-LRRモチーフ遺伝子の探索2012

    • Author(s)
      清水元樹、江部裕介、藤本龍、川邊隆大、Hua Ying、加治誠、Elizabeth Dennis、岡崎桂一
    • Organizer
      日本育種学会第122回講演会
    • Place of Presentation
      京都産業大学
    • Year and Date
      2012-09-15
    • Related Report
      2013 Final Research Report
  • [Presentation] Towards identifying a fusarium wilt resistance gene in Chinese cabbage (Brassica rapa ssp. pekinensis)2012

    • Author(s)
      Tomita H, Shimizu M, Ebe Y, Ying H, Kawanabe T, Kaji M, Fujimoto R, Dennis ES, Okazaki K.
    • Organizer
      10th International Congress on Plant Molecular Biology
    • Place of Presentation
      Jeju, Korea
    • Related Report
      2013 Final Research Report
  • [Presentation] Characterization of NBS-LRR motif genes between S11 and R09 by RNA sequencing in Brassica rapa2012

    • Author(s)
      Shimizu M, Ying H, Fujimoto R, Ebe Y, Tomita H, Kawanabe T, Kaji M, Dennis ES, Okazaki K.
    • Organizer
      10th International Congress on Plant Molecular Biology
    • Place of Presentation
      Jeju, Korea
    • Related Report
      2013 Final Research Report
  • [Presentation] RNA-sequence法を用いたハクサイ品種間(S11×R09)特異的なNBS-LRRモチーフ遺伝子の探索2012

    • Author(s)
      清水元樹
    • Organizer
      日本育種学会
    • Place of Presentation
      京都産業大学
    • Related Report
      2012 Research-status Report
  • [Presentation] Characterization of NBS-LRR motif genes between S11 and R09 by RNA sequencing in Brassica rapa.2012

    • Author(s)
      Motoki Shimizu
    • Organizer
      10th International Congress on Plant Molecular Biology
    • Place of Presentation
      Jeju, Korea
    • Related Report
      2012 Research-status Report
  • [Presentation] Towards identifying a fusarium wilt resistance gene in Chinese cabbage (Brassica rapa ssp. pekinensis).2012

    • Author(s)
      Hiroya Tomita
    • Organizer
      10th International Congress on Plant Molecular Biology
    • Place of Presentation
      Jeju, Korea
    • Related Report
      2012 Research-status Report
  • [Book] The role of DNA methylation in plants2013

    • Author(s)
      Osabe K, Sasaki T, Ishikawa R, Fujimoto R.
    • Publisher
      Nova Science Publisher
    • Related Report
      2013 Final Research Report
  • [Book] DNA Methylation: Principles, Mechanisms and Challenges2013

    • Author(s)
      Kenji Osabe
    • Total Pages
      184
    • Publisher
      Nova publishers
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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