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Functions of brassinosteroid in stress tolerance of rice

Research Project

Project/Area Number 24780005
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Breeding science
Research InstitutionIshikawa Prefectural University

Principal Investigator

SAKAMOTO Tomoaki  石川県立大学, 生物資源環境学部, 准教授 (00345183)

Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywordsイネ / オーキシン / 突然変異体 / 発現制御 / ブラシノステロイド / ユビキチン化 / マイクロアレイ / U-box型E3リガーゼ
Outline of Final Research Achievements

The phytohormones auxin and brassinosteroid are both essential regulators of physiological and developmental processes, and it has been suggested that they act interdependently and synergistically. I showed that auxin stimulates brassinosteroid perception by regulating the amount of brassinosteroid receptors. Next, I found that genetic background influences brassinosteroid-related mutant phenotypes in rice and these phenomena are caused by a transposon insertion into the promoter of brassinosteroid receptor gene. Finally, I isolated a novel positive regulator of brassinosteroid signaling in rice. This protein encodes an U-box E3 ubiquitin ligase, and probably functions in interaction between brassinosteroid and another phytohormone.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (10 results)

All 2014 2013 2012 Other

All Journal Article (8 results) (of which Peer Reviewed: 8 results) Remarks (2 results)

  • [Journal Article] Blue light-promoted rice leaf bending and unrolling are due to up-regulated brassinosteroid biosynthesis genes accompanied by accumulation of castasterone2014

    • Author(s)
      Asahina M, Tamaki Y, Sakamoto T, Shibata K, Nomura T, and Yokota T.
    • Journal Title

      Phytochemistry

      Volume: 104 Pages: 21

    • DOI

      10.1016/j.phytochem.2014.04.017

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Auxins increase expression of the brassinosteroid receptor and brassinosteroid-responsive genes in Arabidopsis2013

    • Author(s)
      Tomoaki Sakamoto and Shozo Fujioka
    • Journal Title

      Plant Signaling & Behavior

      Volume: 8 Issue: 4 Pages: e23509-e23509

    • DOI

      10.4161/psb.23509

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] An E3 ubiquitin ligase, ERECT LEAF1, functions in brassinosteroid signaling of rice2013

    • Author(s)
      Tomoaki Sakamoto, Hidemi Kitano, and Shozo Fujioka
    • Journal Title

      Plant Signaling & Behavior

      Volume: 8 Issue: 11 Pages: e27117-e27117

    • DOI

      10.4161/psb.27117

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Auxin signal transcription factor regulates expression of the brassinosteroid receptor gene in rice2013

    • Author(s)
      Tomoaki Sakamoto
    • Journal Title

      Plant Journal

      Volume: 73 Issue: 4 Pages: 676-688

    • DOI

      10.1111/tpj.12071

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Genetic background influences brassinosteroid-related mutant phenotypes in rice2013

    • Author(s)
      Tomoaki Sakamoto
    • Journal Title

      American Journal of Plant Sciences

      Volume: 4 Issue: 02 Pages: 212-221

    • DOI

      10.4236/ajps.2013.42028

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Characterization of a Tos17 insertion mutant of rice auxin signal transcription factor gene, OsARF24.2013

    • Author(s)
      Sakamoto T(責任著者), and Inukai Y.
    • Journal Title

      American Journal of Plant Sciences

      Volume: 4 Issue: 01 Pages: 84-91

    • DOI

      10.4236/ajps.2013.41013

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] New alleles of rice ebisu dwarf (d2) mutant show both brassinosteroid-deficient and -insensitive phenotypes2012

    • Author(s)
      Tomoaki Sakamoto
    • Journal Title

      American Journal of Plant Sciences

      Volume: 3 Issue: 12 Pages: 1699-1707

    • DOI

      10.4236/ajps.2012.312208

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Rice CYP90D2 and CYP90D3 catalyze C-23 hydroxylation of brassinosteroids in vitro.2012

    • Author(s)
      Sakamoto T(責任著者), Ohnishi T, Fujioka S, Watanabe B, and Mizutani M.
    • Journal Title

      Plant Physiology and Biochemistry

      Volume: 58 Pages: 220-226

    • DOI

      10.1016/j.plaphy.2012.07.011

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Remarks] 全てに強い「スーパー稲」 酷暑、冷夏、塩害(北國新聞掲載記事)

    • URL

      http://www.hokkoku.co.jp/subpage/H20130609101.htm

    • Related Report
      2013 Research-status Report
  • [Remarks] イネの生長を制御する植物ホルモンのはたらきに新たな発見

    • URL

      http://www.ishikawa-pu.ac.jp/news/?p=672

    • Related Report
      2012 Research-status Report

URL: 

Published: 2013-05-31   Modified: 2019-07-29  

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