| Project/Area Number |
24780015
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Crop science/Weed science
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| Research Institution | Kinki University |
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Principal Investigator |
YAMANE Koji 近畿大学, 農学部, 講師 (50580859)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
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| Keywords | ダイズ / 湿害 / 活性酸素 / 電子顕微鏡 |
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| Research Abstract |
We investigated the accumulation of superoxide (O2-) and hydrogen peroxide (H2O2) of soybean roots. Soybeans grown for 1 week were affected by waterlogging for 1 week. One day after removing the stress, tap roots were used for the staining of O2- and H2O2. O2- staining was observed in the middle region of a tap root. The accumulation of H2O2 was detected on plasma membrane in a cortical cell of a tap root. Thus, waterlogging induces the excess accumulation of O2- and O2- derived H2O2 in a cortical cell of a tap root.
We also investigated the growth and yields 98 days after sowing of soybean, which was subjected by waterlogging in an early growth stage. The shoot and root growth, grain and nodule weight, chlorophyll contents and photosynthesis rate of fully uppermost leaves decreased compared with those of the control. These results suggest that waterlogging in the early growth stage induces the decrease in growth and the earlier etiolation of leaves in the late growth stage.
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