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Investigation of novel florigen function of over-wintering buds in perennial plant

Research Project

Project/Area Number 24780030
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Horticulture/Landscape architecture
Research InstitutionIwate Biotechnology Research Center

Principal Investigator

IMAMURA Tomohiro  公益財団法人岩手生物工学研究センター, 細胞工学研究部, 研究員 (20468705)

Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywords花卉 / 越冬 / Flowering locus T
Research Abstract

Gentian is a perennial plant and known as ornamental flowers. To reveal the flowering mechanism of gentian, we obtained two FLOWERING LOCUS T (FT) orthologues (GtFT1 and GtFT2), and revealed that GtFTs promote flowering transition in gentian. In the process of GtFT analysis, we found that GtFT1 and GtFT2 are expressed in overwintering buds. Gentian produce overwintering buds, which have strong tolerance to cold, but the mechanisms regulating dormancy are unknown. Recently, FT involved in other aspects of plant development as well as flowering transition. To investigate the function of GtFT in overwintering buds, we searched for the interacted factor of GtFT in overwintering buds. Transgenic gentian plantlets expressing GtFT-sGFP were generated. We performed proteome analysis for this transformant and obtained new interacted factor of GtFT. This result implied that the GtFT mechanism of dormancy is different from in flowering transition.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (11 results)

All 2014 2013 2012 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 1 results) Presentation (7 results) Remarks (2 results)

  • [Journal Article] High concentrations of sucrose induce overwintering bud formation in gentian plantlets cultured in vitro2014

    • Author(s)
      Tomohiro Imamura, Atsumi Higuchi, Ken-Taro Sekine, Tetsuro Yamashita, Hideyuki Takahashi
    • Journal Title

      Plant Biotechnology

      Volume: 31 Issue: 2 Pages: 97-104

    • DOI

      10.5511/plantbiotechnology.13.1211a

    • NAID

      130003391370

    • ISSN
      1342-4580, 1347-6114
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Dehydrins are highly expressed in overwintering buds and enhance drought and freezing tolerance in Gentiana triflora2013

    • Author(s)
      Tomohiro Imamura, Atsumi Higuchi, Hideyuki Takahashi
    • Journal Title

      Plant Science

      Volume: 213 Pages: 55-66

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Presentation] リンドウ越冬組織の環境ストレスの関わるDHN遺伝子の機能解析2013

    • Author(s)
      今村智弘、樋口敦美、高橋秀行
    • Organizer
      第31回日本植物細胞分子生物学会(札幌)大会・シンポジウム
    • Place of Presentation
      北海道大学
    • Related Report
      2013 Final Research Report
  • [Presentation] リンドウ越冬組織の環境ストレスに関わるDHN遺伝子の機能解析2013

    • Author(s)
      今村智弘、樋口敦美、高橋秀行
    • Organizer
      第31回日本植物細胞分子生物学会(札幌)大会
    • Place of Presentation
      北海道大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] Molecular characterization and functional analysis of dehydrin in overwintering buds of alpine plant Gentiana2012

    • Author(s)
      Tomohiro Imamura, Hideyuki Takahashi
    • Organizer
      Plant and Microbe Adaptations to Cold (PMAC) 2012
    • Place of Presentation
      北海道大学
    • Related Report
      2013 Final Research Report 2012 Research-status Report
  • [Presentation] リンドウの越冬に関わるDHN遺伝子の機能解析2012

    • Author(s)
      今村智弘、樋口敦美、高橋秀行
    • Organizer
      第7回メタボロームシンポジウム
    • Place of Presentation
      慶應義塾大学
    • Related Report
      2013 Final Research Report
  • [Presentation] GtDHN enhances drought and cold tolerance in Gentinana triflora2012

    • Author(s)
      Tomohiro Imamura, Atsumi Higuchi, Hideyuki Takahashi
    • Organizer
      10th International Congress on Plant Molecular Biology
    • Place of Presentation
      International Convention Center Jeju, Korea
    • Related Report
      2013 Final Research Report
  • [Presentation] リンドウの越冬に関わるDHN遺伝子の機能解析2012

    • Author(s)
      今村智弘、樋口敦美、高橋秀行
    • Organizer
      第7回メタボロームシンポジウム
    • Place of Presentation
      慶応義塾大学 鶴岡メタボロームキャンパス
    • Related Report
      2012 Research-status Report
  • [Presentation] GtDHN enhances drought and cold tolerance in Gentiana triflora2012

    • Author(s)
      Tomohiro Imamura, Atsumi Higuchi, Hideyuki Takahashi
    • Organizer
      10th International Congress on Plant Molecular Biology
    • Place of Presentation
      International Convention Center Jeju, Korea
    • Related Report
      2012 Research-status Report
  • [Remarks] (益財)岩手生物工学研究センターHP

    • URL

      http://www.ibrc.or.jp/

    • Related Report
      2013 Final Research Report
  • [Remarks] 公益財団法人 岩手生物工学研究センター ホームページ

    • URL

      http://www.ibrc.or.jp/

    • Related Report
      2013 Annual Research Report

URL: 

Published: 2013-05-31   Modified: 2019-07-29  

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