Fine mapping of the Co gene and development of DNA markers controlling columnar growth habit in apple
Project/Area Number |
24780033
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Horticulture/Landscape architecture
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Research Institution | National Agriculture and Food Research Organization |
Principal Investigator |
OKADA Kazuma 独立行政法人農業・食品産業技術総合研究機構, 果樹研究所・リンゴ研究領域, 主任研究員 (10547722)
|
Project Period (FY) |
2012-04-01 – 2014-03-31
|
Project Status |
Completed (Fiscal Year 2013)
|
Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
Keywords | リンゴ / 枝変わり / 樹形 / カラムナー / マッピング / BACライブラリー / 挿入変異 |
Research Abstract |
Apple cultivar 'Wijcik' is a columnar mutant of 'McIntosh'. Columnar growth habit of 'Wijcik' is characterized by compaction of the internodes and reduced lateral shoot growth, and controlled by a single dominant gene, Co, on linkage group 10. In this study, we identified three SSR markers (Mdo.chr10.12, 10.13 and 10.14) that co-segregated with Co by fine mapping. Mdo.chr10.12 and 10.14 are well suited for marker-assisted selection because the sizes of the alleles linked to Co are different from those found in common ancestors of current apple breeding germplasm. We delimited the Co region between SSR markers 11-1 and 14-3, and identified an insertion mutation in the Co region.
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Report
(3 results)
Research Products
(6 results)