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Fine mapping of the Co gene and development of DNA markers controlling columnar growth habit in apple

Research Project

Project/Area Number 24780033
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Horticulture/Landscape architecture
Research InstitutionNational Agriculture and Food Research Organization

Principal Investigator

OKADA Kazuma  独立行政法人農業・食品産業技術総合研究機構, 果樹研究所・リンゴ研究領域, 主任研究員 (10547722)

Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywordsリンゴ / 枝変わり / 樹形 / カラムナー / マッピング / BACライブラリー / 挿入変異
Research Abstract

Apple cultivar 'Wijcik' is a columnar mutant of 'McIntosh'. Columnar growth habit of 'Wijcik' is characterized by compaction of the internodes and reduced lateral shoot growth, and controlled by a single dominant gene, Co, on linkage group 10. In this study, we identified three SSR markers (Mdo.chr10.12, 10.13 and 10.14) that co-segregated with Co by fine mapping. Mdo.chr10.12 and 10.14 are well suited for marker-assisted selection because the sizes of the alleles linked to Co are different from those found in common ancestors of current apple breeding germplasm. We delimited the Co region between SSR markers 11-1 and 14-3, and identified an insertion mutation in the Co region.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (6 results)

All 2014 2013 2012

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (5 results)

  • [Journal Article] Fine mapping of Co, a gene controlling columnar growth habit located on apple (Malus x domestica Borkh.) linkage group 102012

    • Author(s)
      Shigeki Moriya, Kazuma Okada, Takashi Haji, Toshiya Yamamoto, Kazuyuki Abe
    • Journal Title

      Plant Breeding

      Volume: 131 Issue: 5 Pages: 641-647

    • DOI

      10.1111/j.1439-0523.2012.01985.x

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Presentation] リンゴのカラムナー性を制御するCo遺伝子座乗領域における挿入変異の同定2014

    • Author(s)
      岡田和馬,森谷茂樹,藤澤弘子,呉健忠,片寄裕一,藤井浩,寺上伸吾,山本俊哉,阿部和幸
    • Organizer
      園芸学会平成26年度春季大会
    • Place of Presentation
      筑波大学
    • Related Report
      2013 Final Research Report
  • [Presentation] リンゴのカラムナー性を制御するCo 遺伝子座乗領域における挿入変異の同定2014

    • Author(s)
      岡田和馬・森谷茂樹・藤澤弘子・呉健忠・片寄裕一・藤井浩・寺上伸吾・山本俊哉・阿部和幸
    • Organizer
      園芸学会
    • Place of Presentation
      筑波大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] リンゴのカラムナー性を制御するCo遺伝子座乗領域のクローニング2013

    • Author(s)
      岡田和馬,森谷茂樹,藤澤弘子,呉健忠,片寄裕一,寺上伸吾,山本俊哉,阿部和幸
    • Organizer
      園芸学会平成25年度秋季大会
    • Place of Presentation
      岩手大学
    • Related Report
      2013 Final Research Report
  • [Presentation] リンゴのカラムナー性を制御するCo遺伝子座乗領域のクローニング2013

    • Author(s)
      岡田和馬・森谷茂樹・藤澤弘子・呉健忠・片寄裕一・寺上伸吾・山本俊哉・阿部和幸
    • Organizer
      園芸学会
    • Place of Presentation
      岩手大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] リンゴカラムナー性原因遺伝子Coのファインマッピング2012

    • Author(s)
      森谷茂樹,岡田和馬,土師岳,山本俊哉,阿部和幸
    • Organizer
      平成24年度果樹バイテク研究会
    • Place of Presentation
      弘前商工会議所会館
    • Related Report
      2013 Final Research Report 2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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