| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Outline of Final Research Achievements |
Genome sequencing of uncultivable parasitic nematodes is difficult because of limited amount of DNA from isolated individuals. In this study we aimed to develop a novel and efficient method to sequence genome from a single nematode using whole genome amplification (WGA) and a Next generation sequencer (NGS). Based on evaluation of existing results of WGA samples we have designed three new methods of WGA-NGS. We evaluated the three methods in terms of amplification uniformity, SNP distribution and generation of chimera DNA. We found all the methods are useful for SNP detection, but they are different in chimeric DNA generation and a combination of multiple methods can help to achieve better quality of assembly.
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