Visualization and analysis of sex pheromone neural circuits in insects using a novel immediate early gene.
| Project/Area Number |
24780047
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Applied entomology
|
|---|
| Research Institution | Kanazawa University |
|---|
Principal Investigator |
KIYA Taketoshi 金沢大学, 自然システム学系, 特任助教 (90532309)
|
|---|
| Project Period (FY) |
2012-04-01 – 2014-03-31
|
| Project Status |
Completed (Fiscal Year 2013)
|
| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
|
|---|
| Keywords | カイコ / ショウジョウバエ / Hr38 / フェロモン / 神経活動 / 昆虫 / 脳 / 初期応答遺伝子 / カイコガ / NBRE |
|---|
| Research Abstract |
Many insects exhibit stereotypic instinctive behavior, but the underlying neural mechanisms are not well understood due to difficulties in detecting brain activity in freely-moving animals. Immediate early genes (IEGs) whose expression is transiently and rapidly upregulated upon neural activity are powerful tools for detecting behavior-related neural activity. In the present study, I identified Hr38 as a novel IEG that is transiently expressed in the male silkmoth, Bombyx mori, by female odor stimulation. Using Hr38 expression as an indicator of neural activity, I mapped comprehensive activity patterns of the silkmoth brain in response to female sex pheromones. I found that Hr38 can also be used as a neural activity marker in the fly, Drosophila melanogaster. Using Hr38, I constructed a neural activity map of the fly brain in response to female stimulation. In adddition, I established transgenic insects whose active neurons can be visualized with GFP, using Hr38 expression as tools.
|
Report
(3 results)
Research Products
(34 results)
-
-
-
-
[Journal Article] Detection of neural activity in the brains of Japanese honeybee workers during the formation of a "hot defensive bee ball"2012
Author(s)
Ugajin, A., Kiya, T., Kunieda, T., Ono, M., Yoshida, T., Kubo, T.
-
Journal Title
PLoS ONE
Volume: 7(3)
Issue: 3
Pages: e32902-e32902
DOI
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
