Generation of the transgenic silkworm having high ability of RNAi

Research Project

Project/Area Number	24780052
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Applied entomology
Research Institution	National Institute of Agrobiological Sciences
Principal Investigator	KOBAYASHI Isao 独立行政法人農業生物資源研究所, 遺伝子組換えカイコ研究開発ユニット, 研究員 (70442829)
Project Period (FY)	2012-04-01 – 2015-03-31
Project Status	Completed (Fiscal Year 2014)
Budget Amount *help	¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000) Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000) Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000) Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords	カイコ / RNAi / SID-1
Outline of Final Research Achievements	To enhance RNAi efficiency in silkworm, we generated the transgenic silkworm expressing SID-1 and tested RNAi efficiency. The silkworm expresses SID-1 under the Gal4/UAS system constantly. In the experiment of Bmwh3-RNAi, it was known that longer dsRNA was effective in gene knockdown. Obvious phenomena were observed in RNAi experiments of POUM2, Varp, BLOS2, og, Bmok, and XDH2. The iap-dsRNA induced high knockdown in SID-1-expressing silkworm.

Report

(4 results)