Analysis of boron-dependent regulatory mechanism of NIP5;1 mRNA degradation in Arabidopsis thaliana
| Project/Area Number |
24780057
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Plant nutrition/Soil science
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| Research Institution | The University of Tokyo |
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Principal Investigator |
TANAKA Mayuki 東京大学, 農学生命科学研究科, 助教 (80546292)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | シロイヌナズナ / ホウ素 / RNAの安定性 / リボソーム / 輸送体 / 5'-非翻訳領域 / トランスポーター / 転写後制御 / ホウ酸輸送体 / NIP5;1 / upstream uORF / 5'非翻訳領域（5'UTR) / 5' 非翻訳領域 (5'UTR) |
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| Outline of Final Research Achievements |
Purpose of this study is to understand the regulatory mechanisms of Boron-dependent mRNA degradation of NIP5;1, a boric acid channelin Arabisopsis thaliana.
First, in order to identify genes involved in B-dependent NIP5;1 mRNA degradation, I screened M1 plants from an ethyl methanesulphonate-mutagenized population by GFP fluorescence as an indicator. After screening of M2 plants, five individual mutants were obtained. Second, I revealed that ribosomes stall at the minimum upstream ORF, AUGUAA, in the 5’-untranslated region of NIP5;1, in a boron-dependent manner, and this stalling is accompanied by mRNA degradation. It is possibly that this regulation of gene expression through ribosome stalling at AUGUAA is a general system in eukaryote.
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Report
(4 results)
Research Products
(14 results)
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[Journal Article] Comparison of BOR1-like gene expression in two genotypes with different boron efficiencies in commercial crop plants in Thailand2014
Author(s)
Leaungthitikanchana, S., Tanaka, M., Lordkaew, S., Jamjod, S., Rerkasem, B. and Fujiwara, T.
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Journal Title
Soil Science and Plant Nutrition
Volume: 60
Issue: 3
Pages: 333-340
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] OsNIP3;1, a rice boric acid channel, regulates boron distribution and is essential for growth under boron-deficient conditions.2014
Author(s)
Hanaoka, H., Uraguchi, S., Takano, J., Tanaka, M. and Fujiwara, T.
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Journal Title
The Plant Journal
Volume: 78
Issue: 5
Pages: 890-902
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Differential expression of three BOR1 genes corresponding to different genomes in response to boron conditions in hexaploid Wheat (Triticum aestivum L.).2013
Author(s)
Leaungthitikanchana, S, Fujibe, T, Tanaka, M, Wang, S, Sotta, N, Takano, J, Fujiwara, T.
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Journal Title
Plant and Cell Physiology
Volume: 54
Issue: 7
Pages: 1056-63
DOI
NAID
Related Report
Peer Reviewed / Open Access
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[Presentation] Mayuki Tanaka2012
Author(s)
Boron-dependent mRNA degradation of NIP5;1, boric acid channel, through 5’UTR in Arabidopsis thaliana roots
Organizer
Frontiers in Plant RNA Research 2012
Place of Presentation
北海道札幌市 北海道大学札幌キャンパス
Related Report
Invited
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[Presentation] 田中 真幸2012
Author(s)
植物のホウ素輸送体遺伝子のホウ素に応答したmRNA蓄積の制御機構の解析
Organizer
第7回トランスポーター研究会年会/JTRA2012
Place of Presentation
京都府京都市 京都大学農学部 (京都大学吉田キャンパス北部構内）
Related Report
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