| Project/Area Number |
24780057
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Plant nutrition/Soil science
|
|---|
| Research Institution | The University of Tokyo |
|---|
Principal Investigator |
TANAKA Mayuki 東京大学, 農学生命科学研究科, 助教 (80546292)
|
|---|
| Project Period (FY) |
2012-04-01 – 2015-03-31
|
| Project Status |
Completed (Fiscal Year 2014)
|
| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
|---|
| Keywords | シロイヌナズナ / ホウ素 / RNAの安定性 / リボソーム / 輸送体 / 5'-非翻訳領域 / トランスポーター / 転写後制御 / ホウ酸輸送体 / NIP5;1 / upstream uORF / 5'非翻訳領域（5'UTR) / 5' 非翻訳領域 (5'UTR) |
|---|
| Outline of Final Research Achievements |
Purpose of this study is to understand the regulatory mechanisms of Boron-dependent mRNA degradation of NIP5;1, a boric acid channelin Arabisopsis thaliana.
First, in order to identify genes involved in B-dependent NIP5;1 mRNA degradation, I screened M1 plants from an ethyl methanesulphonate-mutagenized population by GFP fluorescence as an indicator. After screening of M2 plants, five individual mutants were obtained. Second, I revealed that ribosomes stall at the minimum upstream ORF, AUGUAA, in the 5’-untranslated region of NIP5;1, in a boron-dependent manner, and this stalling is accompanied by mRNA degradation. It is possibly that this regulation of gene expression through ribosome stalling at AUGUAA is a general system in eukaryote.
|
