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Isolation and Characterization of STOP1 Ortholog Genes of Tea Plant (Camellia sinensis L.)

Research Project

Project/Area Number 24780059
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Plant nutrition/Soil science
Research InstitutionShizuoka University

Principal Investigator

IKKA Takashi  静岡大学, (連合)農学研究科(研究院), 助教 (90580647)

Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywordsチャ / アルミニウム耐性 / STOP1転写因子
Outline of Final Research Achievements

Aluminum (Al) and proton (H+) tolerances are essential traits for plants to adapt to acid soil environments. Recently, in Arabidopsis (A. thaliana), it was reported that these tolerances are mediated by a zinc-finger transcription factor, sensitive to proton rhizotoxicity1 (AtSTOP1), which regulates the transcription of multiple genes critical for both tolerances. Tea plants (Camellia sinensis L.) grow well in strongly acidic soils (less than pH 4) containing high levels of Al3+ and H+. However, the mechanisms of both tolerances by tea plants are still poorly clarified. Here, we isolated two STOP1 orthologs (CsSTOP1 and CsSTOP2) from tea plant, and characterized in planta complementation assays. By using CsSTOP1- or CsSTOP2-expressing Arabidopsis transgenic lines, it was suggested that several genes, known to be regulated in the AtSTOP1 under Al3+ and H+ treatments, were also regulated in the CsSTOP1 and CsSTOP2.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (7 results)

All 2015 2014 2013 2012

All Journal Article (1 results) Presentation (6 results)

  • [Journal Article] Isolation and Characterization of STOP1 Ortholog Genes of Tea Plant (Camellia sinensis L.)2014

    • Author(s)
      Tomoko Matsuda,Yoshinao Ohyama,Hiroyuki Koyama,Chiemi Iwata,Akio Morita,Takashi Ikka
    • Journal Title

      Proceedings of the 5th International Conference on O-CHA (Tea) Culture and Science.

      Volume: ICOS2013

    • Related Report
      2013 Research-status Report
  • [Presentation] チャ (Camellia sinensis L.) 培養根を用いたアルミニウム誘導性有機酸放出機構の解析2015

    • Author(s)
      田中靖乃,久保智也,片山博史,一家崇志,森田明雄
    • Organizer
      茶学術研究会
    • Place of Presentation
      静岡県静岡市ホテルアソシア静岡
    • Year and Date
      2015-03-17
    • Related Report
      2014 Annual Research Report
  • [Presentation] チャ培養根を用いたアルミニウム誘導性有機酸放出機構の解析2015

    • Author(s)
      田中靖乃,久保智也,片山博史,森田明雄,一家崇志
    • Organizer
      静岡ライフサイエンスシンポジウム
    • Place of Presentation
      静岡県静岡市静岡大学
    • Year and Date
      2015-03-07
    • Related Report
      2014 Annual Research Report
  • [Presentation] チャ (Camellia sinensis L.) のSTOP1相同遺伝子の解析2014

    • Author(s)
      田中靖乃,一家崇志,松田知子,小林佑理子,井内聖,小林正智,小山博之,森田明雄
    • Organizer
      中部土壌肥料学会
    • Place of Presentation
      福井県敦賀市プラザ萬象
    • Year and Date
      2014-11-13
    • Related Report
      2014 Annual Research Report
  • [Presentation] チャ (Camellia sinensis L.) のSTOP1相同遺伝子の単離と機能解析2014

    • Author(s)
      松田知子,大山義慶,小山博之,岩田千衣美,森田明雄,一家崇志
    • Organizer
      茶学術研究会
    • Place of Presentation
      ホテルアソシア静岡
    • Related Report
      2013 Research-status Report
  • [Presentation] Isolation and Characterization of STOP1 Ortholog Genes of Tea Plant (Camellia sinensis L.)2013

    • Author(s)
      Tomoko Matsuda,Yoshinao Ohyama,Hiroyuki Koyama,Chiemi Iwata,Akio Morita,Takashi Ikka
    • Organizer
      The 5th International Conference on O-CHA (Tea) Culture and Science.
    • Place of Presentation
      グランシップ静岡
    • Related Report
      2013 Research-status Report
  • [Presentation] 相補組換えシロイヌナズナを用いたSTOP1の機能解析2012

    • Author(s)
      大山慶直,小林佑理子,井内聖,小林正智,一家崇志,小山博之
    • Organizer
      日本土壌肥料学会
    • Place of Presentation
      鳥取大学(鳥取県)
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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