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Generation of transformants showing mineral-stress tolerance based on the modification of light-harvesting function

Research Project

Project/Area Number 24780060
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Plant nutrition/Soil science
Research InstitutionTokyo University of Agriculture

Principal Investigator

SAITO Akihiro  東京農業大学, 応用生物科学部, 助教 (10610355)

Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Keywordsオオムギ / 集光性アンテナタンパク質 / 鉄欠乏 / 強光 / 形質転換 / 耐性作物 / タンパク質リン酸化 / 集光性アンテナ / Lhcb1 / アイソフォーム / チラコイド膜 / 光合成 / イネ
Research Abstract

There are many iron-containing proteins working in photosynthetic electron transport chain so that iron deficiency causes the imbalance in the electron transport between photosystem II (PSII) and PSI. We have recently found that iron-deficiency specifically induces a gene of the LHCII family that encodes a homolog of Lhcb1 in barley (Hordeum vulgare), a plant that is tolerant to Fe deficiency. In this study, we demonstrate that a large proportion of HvLhcb1 was stably phosphorylated and located adjacent to PSI rather than PSII under Fe-deficient conditions. These results suggested that the phosphorylation of HvLhcb1 in Fe-deficient barley induces the disconnection of the antenna from the PSII core and migration the protein to PSI, resulting the optimization of the electron transport rate. Finally, we genetically engineered rice plants to express the HvLhcb1 gene and evaluated whether the gene confers tolerance to plants and/or improves plant productivity under various environments.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (51 results)

All 2014 2013 2012 Other

All Journal Article (4 results) (of which Peer Reviewed: 4 results) Presentation (45 results) Remarks (2 results)

  • [Journal Article] Fe deficiency induces phosphorylation and translocation of Lhcb1 in barley thylakoid membranes2014

    • Author(s)
      Akihiro Saito, Mizuho Shimizu, Hitomi Nakamura, Shoko Maeno, Eitaro Miwa, Kyoko Higuchi and Kintake Sonoike
    • Journal Title

      FEBS Letters

      Volume: Volume 588, Issue 12 Pages: 2042-2048

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Early senescence of the oldest leaves of Fe-deficient barley plants may contribute to phytosiderophore release from the roots2014

    • Author(s)
      Kyoko Higuchi, Jun Iwase, Yoshifumi Tsukiori, Daiki Nakura, Nahoko Kobayashi, Hidenori Ohashi, Akihiro Saito and Eitaro Miwa
    • Journal Title

      Physiologia Plantarum

      Volume: 10.1111 Pages: 12175-12175

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Fe deficiency induces phosphorylation and translocation of Lhcb1 in barley thylakoid membranes2014

    • Author(s)
      Akihiro Saito, Mizuho Shimizu, Hitomi Nakamura, Shoko Maeno, Riko Katase, Eitaro Miwa, Kyoko Higuchi and Kintake Sonoike
    • Journal Title

      FEBS Letters

      Volume: in press Issue: 12 Pages: 2042-2048

    • DOI

      10.1016/j.febslet.2014.04.031

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Early senescence of the oldest leaves of Fe-deficient barley plants may contribute to phytosiderophore release from the roots2014

    • Author(s)
      Kyoko Higuchi, Jun Iwase, Yoshifumi Tsukiori, Daiki Nakura, Nahoko Kobayashi, Hidenori Ohashi, Akihiro Saito, Eitaro Miwa
    • Journal Title

      Physiologia Plantarum

      Volume: in press Issue: 3 Pages: 313-322

    • DOI

      10.1111/ppl.12175

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Presentation] 鉄欠乏オオムギ光化学系の含鉄タンパク質蓄積パターンの特徴(PF036)2014

    • Author(s)
      富澤隆章、齋藤彰宏、樋口恭子
    • Organizer
      第55回日本植物生理学会年会
    • Place of Presentation
      富山大学五福キャンパス
    • Related Report
      2013 Final Research Report
  • [Presentation] 鉄欠乏オオムギの葉緑体における鉄の分布(PF037)2014

    • Author(s)
      小川智美、齋藤彰宏、樋口恭子
    • Organizer
      第55回日本植物生理学会年会
    • Place of Presentation
      富山大学五福キャンパス
    • Related Report
      2013 Final Research Report
  • [Presentation] 鉄欠乏オオムギにおけるソース・シンクの代謝適応応答(PF041)2014

    • Author(s)
      岩瀬潤、月居佳史、名倉大貴、齋藤彰宏、三輪睿太郎、樋口恭子
    • Organizer
      第55回日本植物生理学会年会
    • Place of Presentation
      富山大学五福キャンパス
    • Related Report
      2013 Final Research Report
  • [Presentation] 鉄欠乏オオムギ下位葉の老化に伴って早期に発現が誘導される遺伝子の解析(PF084)2014

    • Author(s)
      樋口恭子、小林奈保子、内田裕也、齋藤彰宏
    • Organizer
      第55回日本植物生理学会年会
    • Place of Presentation
      富山大学五福キャンパス
    • Related Report
      2013 Final Research Report
  • [Presentation] 鉄欠乏耐性作物オオムギにおけるスーパーオキシドディスムターゼ(SOD)の鉄欠乏による誘導(PF225)2014

    • Author(s)
      樋口恭子、尾畑玲、齋藤彰宏
    • Organizer
      第55回日本植物生理学会年会
    • Place of Presentation
      富山大学五福キャンパス
    • Related Report
      2013 Final Research Report
  • [Presentation] 鉄欠乏オオムギ光化学系の含鉄タンパク質蓄積パターンの特徴2014

    • Author(s)
      富澤隆章、齋藤彰宏、樋口恭子
    • Organizer
      日本植物生理学会年会
    • Place of Presentation
      富山大学・五福キャンパス
    • Related Report
      2013 Annual Research Report
  • [Presentation] 鉄欠乏オオムギの葉緑体における鉄の分布2014

    • Author(s)
      小川智美、齋藤彰宏、樋口恭子
    • Organizer
      日本植物生理学会年会
    • Place of Presentation
      富山大学・五福キャンパス
    • Related Report
      2013 Annual Research Report
  • [Presentation] 鉄欠乏オオムギにおけるソース・シンクの代謝適応応答2014

    • Author(s)
      岩瀬潤、月居佳史、名倉大貴、齋藤彰宏、三輪睿太郎、樋口恭子
    • Organizer
      日本植物生理学会年会
    • Place of Presentation
      富山大学・五福キャンパス
    • Related Report
      2013 Annual Research Report
  • [Presentation] 鉄欠乏オオムギ下位葉の老化に伴って早期に発現が誘導される遺伝子の解析2014

    • Author(s)
      樋口恭子、小林奈保子、内田裕也、齋藤彰宏
    • Organizer
      日本植物生理学会年会
    • Place of Presentation
      富山大学・五福キャンパス
    • Related Report
      2013 Annual Research Report
  • [Presentation] 鉄欠乏耐性作物オオムギにおけるスーパーオキシドディスムターゼ(SOD)の鉄欠乏による誘導2014

    • Author(s)
      樋口恭子、尾畑玲、齋藤彰宏
    • Organizer
      日本植物生理学会年会
    • Place of Presentation
      富山大学・五福キャンパス
    • Related Report
      2013 Annual Research Report
  • [Presentation] Comprehensive mechanism for adaptation of barley to Fe deficiency2013

    • Author(s)
      Kyoko Higuchi, Akihiro Saito
    • Organizer
      17th International Plant Nutrition Colloquium (IPNC-2013)
    • Place of Presentation
      Istanbul Convention and Exhibition Center, Istanbul, Turkey
    • Year and Date
      2013-08-19
    • Related Report
      2013 Final Research Report
  • [Presentation] 光ストレス誘導型HvLhcb1遺伝子を導入したイネの収量改善効果2013

    • Author(s)
      齋藤彰宏、加藤克紀、中島朋美、三輪睿太郎、樋口恭子
    • Organizer
      日本土壌肥料学会2013年年次大会
    • Place of Presentation
      名古屋大学・東山キャンパス
    • Related Report
      2013 Final Research Report
  • [Presentation] イネ科作物の鉄欠乏適応と同化産物分配パターンの関係2013

    • Author(s)
      樋口恭子、岩瀬潤、月居佳史、名倉大貴、大橋英典、齋藤彰宏、三輪睿太郎
    • Organizer
      日本土壌肥料学会2013年年次大会
    • Place of Presentation
      名古屋大学・東山キャンパス
    • Related Report
      2013 Final Research Report
  • [Presentation] Supply of assimilates by old leaves contributes to barley growth under Fe deficiency2013

    • Author(s)
      Jun Iwase, Daiki Nagura, Yoshihumi Tsukiori, Akihiro Saito, Eitaro Miwa, Kyoko Higuchi
    • Organizer
      17th International Plant Nutrition Colloquium (IPNC-2013)
    • Place of Presentation
      Istanbul Convention and Exhibition Center, Istanbul, Turkey
    • Related Report
      2013 Final Research Report
  • [Presentation] Ectopic expression of photooxidative stress-inducible barley gene, Lhcb1, in rice affects growth and photosynthetic responses2013

    • Author(s)
      Akihiro Saito, Katsuki Kato, Shoko Maeno, Tomoko Nakajima, Eitaro Miwa, Kyoko Higuchi
    • Organizer
      17th International Plant Nutrition Colloquium (IPNC-2013)
    • Place of Presentation
      Istanbul Convention and Exhibition Center, Istanbul, Turkey
    • Related Report
      2013 Final Research Report
  • [Presentation] Phosphorylation of Lhcb1 mediated by cytochrome b_6f is crucial for photosynthetic acclimation ot Fe deficiency in barley2013

    • Author(s)
      Akihiro Saito, Kyoko Higuchi, Kintake Sonoike
    • Organizer
      16th International Congress on Photosynthesis Research
    • Place of Presentation
      Hyatt Regency at the Arch, St.Louis, MO, USA
    • Related Report
      2013 Final Research Report
  • [Presentation] 鉄栄養状態で変動するオオムギ集光性アンテナLhcb1アイソフォームの解析(PF027)2013

    • Author(s)
      齋藤彰宏・片瀬莉子・前野祥子・中島朋美・三輪睿太郎・樋口恭子
    • Organizer
      第54回日本植物生理学会年会
    • Place of Presentation
      岡山大学津島キャンパス
    • Related Report
      2013 Final Research Report
  • [Presentation] イネ科作物の鉄欠乏適応と同化産物分配パターンの関係2013

    • Author(s)
      樋口恭子、岩瀬潤、月居佳史、名倉大貴、大橋英典、齋藤彰宏、三輪睿太郎
    • Organizer
      日本土壌肥料学会名古屋大会
    • Place of Presentation
      名古屋大学・東山キャンパス
    • Related Report
      2013 Annual Research Report
  • [Presentation] 光ストレス誘導型HvLhcb1遺伝子を導入したイネの収量改善効果2013

    • Author(s)
      齋藤彰宏、加藤克紀、中島朋美、三輪睿太郎、樋口恭子
    • Organizer
      日本土壌肥料学会名古屋大会
    • Place of Presentation
      名古屋大学・東山キャンパス
    • Related Report
      2013 Annual Research Report
  • [Presentation] Phosphorylation of Lhcb1 mediated by cytochrome b6f is crucial for photosynthetic acclimation to Fe deficiency in barley2013

    • Author(s)
      Akihiro Saito, Kyoko Higuchi, Kintake Sonoike
    • Organizer
      16th International Congress on Photosynthesis Research
    • Place of Presentation
      St. Louis, MO, USA, Hyatt Regency at the Arch
    • Related Report
      2013 Annual Research Report
  • [Presentation] Ectopic expression of the photooxidative stress-inducible barley gene, Lhcb1, in rice affects growth and photosynthetic responses2013

    • Author(s)
      Akihiro Saito, Katsuki Kato, Shoko Maeno, Tomoko Nakajima, Eitaro Miwa and Kyoko Higuchi
    • Organizer
      17th International Plant Nutrition Colloquium
    • Place of Presentation
      Istanbul, Turkey, at the Istanbul Convention and Exhibition Center
    • Related Report
      2013 Annual Research Report
  • [Presentation] mechanisms for adaptation of barley to Fe deficiency2013

    • Author(s)
      Kyoko Higuchi, Akihiro SaitoComprehensive
    • Organizer
      17th International Plant Nutrition Colloquium
    • Place of Presentation
      Istanbul, Turkey, at the Istanbul Convention and Exhibition Center
    • Related Report
      2013 Annual Research Report
  • [Presentation] Supply of assimilates by old leaves contributes to barley growth under Fe deficiency2013

    • Author(s)
      Jun Iwase, Daiki Nagura, Yoshihumi Tsukiori, Akihiro Saito, Eitaro Miwa, Kyoko Higuchi
    • Organizer
      17th International Plant Nutrition Colloquium
    • Place of Presentation
      Istanbul, Turkey, at the Istanbul Convention and Exhibition Center
    • Related Report
      2013 Annual Research Report
  • [Presentation] 鉄栄養状態で変動するオオムギ集光性アンテナLhcb1アイソフォームの解析2013

    • Author(s)
      齋藤彰宏、片瀬莉子、前野祥子、中島朋美、三輪睿太郎、樋口恭子
    • Organizer
      第54回日本植物生理学会
    • Place of Presentation
      岡山大学津島キャンパス
    • Related Report
      2012 Research-status Report
  • [Presentation] イネの花粉特異的なホウ素輸送体、OsBOR4の機能解析2013

    • Author(s)
      田中伸裕・浦口晋平・齋藤彰宏・梶川昌孝・笠井光治・藤原徹
    • Organizer
      第54回日本植物生理学会
    • Place of Presentation
      岡山大学津島キャンパス
    • Related Report
      2012 Research-status Report
  • [Presentation] 原形質ニッケルの効率的な排除に関わる未知小胞の機能解析2013

    • Author(s)
      山口萌・齋藤彰宏・三輪睿太郎・樋口恭子
    • Organizer
      第54回日本植物生理学会
    • Place of Presentation
      岡山大学津島キャンパス
    • Related Report
      2012 Research-status Report
  • [Presentation] Comprehensive mechanisms for adaptation of barley to Fe deficiency2013

    • Author(s)
      Kyoko Higuchi, Akihiro Saito
    • Organizer
      17th International Plant Nutrition Colloquium (IPNC-2013)
    • Place of Presentation
      Istanbul Convention and Exhibition Center, Turkey
    • Related Report
      2012 Research-status Report
  • [Presentation] Ectopic expression of the photooxidative stress-inducible barley gene, Lhcb1, in rice affects growth and photosynthetic responses.2013

    • Author(s)
      Akihiro Saito, Katsuki Kato, Shoko Maeno, Tomoko Nakajima, Eitaro Miwa, Kyoko Higuchi
    • Organizer
      17th International Plant Nutrition Colloquium (IPNC-2013)
    • Place of Presentation
      Istanbul Convention and Exhibition Center, Turkey
    • Related Report
      2012 Research-status Report
  • [Presentation] 光ストレス誘導型HvLhcb1遺伝子を導入したイネの収量改善効果2013

    • Author(s)
      齋藤彰宏・加藤克紀・中島朋美・三輪睿太郎・樋口恭子
    • Organizer
      日本土壌肥料学会2013年 年次大会
    • Place of Presentation
      名古屋大学東山キャンパス
    • Related Report
      2012 Research-status Report
  • [Presentation] イネ科作物の鉄欠乏適応と同化産物分配パターンの関係2013

    • Author(s)
      樋口恭子、岩瀬潤、月居佳史、名倉大樹、大橋英典、齋藤彰宏、三輪睿太郎
    • Organizer
      日本土壌肥料学会2013年 年次大会
    • Place of Presentation
      名古屋大学東山キャンパス
    • Related Report
      2012 Research-status Report
  • [Presentation] 光酸化ストレス応答性のオオムギ集光性アンテナHvLhcb1.12の機能解析2012

    • Author(s)
      齋藤彰宏・加藤克紀・清水瑞穂・中村仁美・三輪睿太郎・樋口恭子
    • Organizer
      日本植物学会第76回大会
    • Place of Presentation
      兵庫県立大学書写キャンパス
    • Related Report
      2013 Final Research Report
  • [Presentation] 鉄欠乏条件下におけるオオムギの各ソース葉からシンク器官への転流2012

    • Author(s)
      岩瀬潤・齋藤彰宏・三輪睿太郎・樋口恭子
    • Organizer
      日本土壌肥料学会2012年年次大会
    • Place of Presentation
      鳥取大学鳥取キャンパス
    • Related Report
      2013 Final Research Report
  • [Presentation] オオムギ由来の集光性アンテナを導入したイネ形質転換体の栄養ストレス適応2012

    • Author(s)
      齋藤彰宏・加藤克紀・清水瑞穂・中村仁美・三輪睿太郎・樋口恭子
    • Organizer
      日本土壌肥料学会2012年年次大会
    • Place of Presentation
      鳥取大学鳥取キャンパス
    • Related Report
      2013 Final Research Report
  • [Presentation] Allocation of assimilated C in barley grown under Fe-deficient condition2012

    • Author(s)
      Kyoko Higuchi, Akihiro Saito
    • Organizer
      16th International Symposium on Iron Nutrition and Interactions in Plants (ISINIP-2012)
    • Place of Presentation
      UMass Conference Center, Amherst, MA, USA
    • Related Report
      2013 Final Research Report
  • [Presentation] Metabolic adaptation to Fe deficiency in barley2012

    • Author(s)
      Kyoko Higuchi, Akihiro Saito
    • Organizer
      16th International Symposium on Iron Nutrition and Interactions in Plants (ISINIP-2012)
    • Place of Presentation
      UMass Conference Center, Amherst, MA, USA
    • Related Report
      2013 Final Research Report
  • [Presentation] Functional validation of a long-term iron deficiency-inducible Lhcb1 isoform in transgenic rice plants2012

    • Author(s)
      Akihiro Saito, Katsuki Kato, Mizuho Shimizu, Hitomi Nakamura, Kyoko Higuchi
    • Organizer
      16th International Symposium on Iron Nutrition and Interactions in Plants (ISINIP-2012)
    • Place of Presentation
      UMass Conference Center, Amherst, MA, USA
    • Related Report
      2013 Final Research Report
  • [Presentation] Photoadaptation and quality control of photosystem II in Fe-deficient barley leaves2012

    • Author(s)
      Akihiro Saito, Katsuki Kato, Mizuho Shimizu, Hitomi Nakamura, Kyoko Higuchi
    • Organizer
      16th International Symposium on Iron Nutrition and Interactions in Plants (ISINIP-2012)
    • Place of Presentation
      UMass Conference Center, Amherst, MA, USA
    • Related Report
      2013 Final Research Report
  • [Presentation] Photoadaptation and quality control of photosystem II in Fe-deficient barley leaves.2012

    • Author(s)
      Akihiro Saito, Katsuki Kato, Mizuho Shimizu, Hitomi Nakamura, Kyoko Higuchi
    • Organizer
      16th International Symposium on Iron Nutrition and Interactions in Plants (ISINIP-2012)
    • Place of Presentation
      UMass Conference Center, Amherst, USA
    • Related Report
      2012 Research-status Report
  • [Presentation] Functional validation of a long-term iron deficiency-inducible Lhcb1 isoform in transgenic rice plants.2012

    • Author(s)
      Akihiro Saito, Katsuki Kato, Mizuho Shimizu, Hitomi Nakamura, Kyoko Higuchi
    • Organizer
      16th International Symposium on Iron Nutrition and Interactions in Plants (ISINIP-2012)
    • Place of Presentation
      UMass Conference Center, Amherst, USA
    • Related Report
      2012 Research-status Report
  • [Presentation] Metabolic adaptation to Fe deficiency in barley.2012

    • Author(s)
      Kyoko Higuchi, Akihiro Saito
    • Organizer
      16th International Symposium on Iron Nutrition and Interactions in Plants (ISINIP-2012)
    • Place of Presentation
      UMass Conference Center, Amherst, USA
    • Related Report
      2012 Research-status Report
  • [Presentation] Allocation of assimilated C in barley grown under Fe-deficient condition.2012

    • Author(s)
      Kyoko Higuchi, Akihiro Saito
    • Organizer
      16th International Symposium on Iron Nutrition and Interactions in Plants (ISINIP-2012)
    • Place of Presentation
      UMass Conference Center, Amherst, USA
    • Related Report
      2012 Research-status Report
  • [Presentation] オオムギ由来の集光性アンテナを導入したイネ形質転換体の栄養ストレス適応2012

    • Author(s)
      齋藤彰宏・加藤克紀・清水瑞穂・中村仁美・三輪睿太郎・樋口恭子
    • Organizer
      日本土壌肥料学会2012年年次大会
    • Place of Presentation
      鳥取大学
    • Related Report
      2012 Research-status Report
  • [Presentation] 鉄欠乏条件下におけるオオムギの各ソース葉からシンク器官への転流2012

    • Author(s)
      岩瀬潤・齋藤彰宏・三輪睿太郎・樋口恭子
    • Organizer
      日本土壌肥料学会2012年 年次大会
    • Place of Presentation
      鳥取大学
    • Related Report
      2012 Research-status Report
  • [Presentation] 光酸化ストレス応答性のオオムギ集光性アンテナHvLhcb1.12の機能解析2012

    • Author(s)
      齋藤彰宏・加藤克紀・清水瑞穂・中村仁美・三輪睿太郎・樋口恭子
    • Organizer
      日本植物学会 第76回大会
    • Place of Presentation
      兵庫県立大学書写キャンパス
    • Related Report
      2012 Research-status Report
  • [Presentation] タバコBY-2培養細胞のニッケル集積に関与する細胞内膜系の解析2012

    • Author(s)
      山口萌・齋藤彰宏・三輪睿太郎・樋口恭子
    • Organizer
      日本植物学会 第76大会
    • Place of Presentation
      兵庫県立大学書写キャンパス
    • Related Report
      2012 Research-status Report
  • [Remarks]

    • URL

      http://www.nodai.ac.jp/app/original/02shokubutsu/index.html

    • Related Report
      2013 Final Research Report
  • [Remarks] 東京農業大学生物応用化学科 植物生産化学研究室

    • URL

      http://www.nodai.ac.jp/app/original/02shokubutsu/index.html

    • Related Report
      2012 Research-status Report

URL: 

Published: 2013-05-31   Modified: 2019-07-29  

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