Regulatory mechanism of strigolactone production under nitrogen deficiency
| Project/Area Number |
24780061
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Plant nutrition/Soil science
|
|---|
| Research Institution | Toyo University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2012-04-01 – 2014-03-31
|
| Project Status |
Completed (Fiscal Year 2013)
|
| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
|
|---|
| Keywords | ストリゴラクトン / 窒素欠乏 / イネ / LC-MS/MS / 生合成 / マイクロアレイ / 質量分析 |
|---|
| Research Abstract |
SL analysis by LC-MS/MS showed that SL levels elevated in response to nitrogen deficiency in rice. SL-related gene expression analysis by qRT-PCR demonstrated that SL biosynthetic genes (D10, D17, D27, and OsMAX1s) highly expressed under nitrogen deficiency, but not SL signal transduction genes (D3 and D14). We propose that rice does not change the sensitivity of SL perception but increases SL production in response to nitrogen deficiency. In addition, we performed microarray to find new SL biosynthetic and metabolism genes, and screened genes that showed the expression pattern similar to SL-biosynthetic genes.
|
Report
(3 results)
Research Products
(12 results)
