Characterization of a set of novel bZIP transcription factors involved in nitrogen-starvation responses in plants

• Project/Area Number: 24780065
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Plant nutrition/Soil science
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: KIBA TAKATOSHI 独立行政法人理化学研究所, 環境資源科学研究センター, 研究員 (20532097)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 植物分子生理学 / 窒素栄養 / 窒素欠乏 / 硝酸イオン輸送体 / 転写因子 / 高親和性硝酸イオン輸送体 / 転写抑制因子 / 植物栄養 / Myb型転写因子 / 転写抑制

Research Abstract

I identified a set of GARP transcription factors as regulators of a high-affinity nitrate transporter gene AtNRT2.4, which is crucial for optimal adaptation to nitrogen starvation. I demonstrated that the GARP transcription factors act as transcriptional repressors to suppress AtNRT2.4 expression in nitrogen-replete conditions.

Research Products

• [Journal Article] Effects of high CO2 on growth and metabolism of Arabidopsis seedlings during growth with a constantly limited supply of nitrogen (2014)
  • Author(s): Takatani, N., Ito, T., Kiba, T., Mori, M. Miyamoto, T., Maeda, S., Omata, T.
  • Journal Title: Plant and Cell Physiology Volume: 55 Issue: 2 Pages: 281-292
  • DOI: 10.1093/pcp/pct186
  • Peer Reviewed
• [Journal Article] Side-chain modification of cytokinins control shoot growth in Arabidopsis (2013)
  • Author(s): Kiba, T., Takei, K., Kojima, M. and Sakakibara, H.
  • Journal Title: Developmental Cell Volume: 27 Issue: 4 Pages: 452-461
  • DOI: 10.1016/j.devcel.2013.10.004
  • Peer Reviewed
• [Journal Article] The Arabidopsis nitrate transporter NRT2.4 plays a double role in roots and shoots of nitrogen-starved plants (2012)
  • Author(s): Kiba, T., Feria-Bourrellier, A.-B., Lafouge, F., Lezhneva, L., Boutet-Mercey, S., Orsel, M., Brehaut, V., Miller, A., Daniel-Vedele, F., Sakakibara, H. and Krapp, A.
  • Journal Title: Plant Cell Volume: 24 Issue: 1 Pages: 245-258
  • DOI: 10.1105/tpc.111.092221
  • Peer Reviewed
• [Journal Article] Transcriptional repressor PRR5 directly regulates clock-output pathways. (2012)
  • Author(s): Nakamichi et. al.,
  • Journal Title: PNAS Volume: 109 Issue: 42 Pages: 17123-17128
  • DOI: 10.1073/pnas.1205156109
  • Peer Reviewed
• [Journal Article] The response and recovery of the Arabidopsis thaliana transcriptome to phosphate starvation (2012)
  • Author(s): Jongchan Woo, Cameron R MacPherson, Jun Liu, Huan Wang, Takatoshi Kiba, Matthew Hannah, Xiu-Jie Wang, Vladimir B Bajic and Nam-Hai Chua
  • Journal Title: BMC Plant Biol Volume: 12 Issue: 1
  • DOI: 10.1186/1471-2229-12-62
  • Peer Reviewed
• [Journal Article] Arabidopsis lonely guy (LOG) multiple mutants reveal a central role of the LOG-dependent pathway in cytokinin activation (2012)
  • Author(s): Tokunaga,H., Kojima, M., Kuroha, T., Ishida, T., Sugimoto, K., Kiba, T. and Sakakibara, H.
  • Journal Title: Plant Journal Volume: 69 Issue: 2 Pages: 355-365
  • DOI: 10.1111/j.1365-313x.2011.04795.x
  • Peer Reviewed
• [Presentation] Françoise Daniel-Vedele, Anne Krapp. The nitrate transporter NRT2.5 plays a major role in nitrate acquisition in nitrogen-starved adult Arabidopsis (2014)
  • Author(s): Takatoshi Kiba, Lina Lezhneva, Ana Belen Feria-Bourellier, Florence Lafouge, Stéphanie Boutet-Mercey, Nino Niccolo, Hitoshi Sakakibara
  • Organizer: 日本植物生理学会年会
  • Place of Presentation: 富山市
  • Year and Date: 2014-03-19
• [Presentation] The nitrate transporter NRT2.5 plays a major role in nitrate acquisition in nitrogen-starved adult Arabidopsis (2014)
  • Author(s): Takatoshi Kiba, Lina Lezhneva, Ana Belen Feria-Bourellier, Florence Lafouge, Stephanie Boutet-Mercey, Nino Niccolo, Hitoshi Sakakibara, Francoise Daniel-Vedele, Anne Krapp
  • Organizer: 日本植物生理学会
  • Place of Presentation: 富山大学、富山県
• [Presentation] Françoise Daniel-Vedele, Anne Krapp (2013)
  • Author(s): Takatoshi Kiba, Lina Lezhneva, Ana Belen Feria-Bourellier, Florence Lafouge, Stéphanie Boutet-Mercey, Nino Niccolo, Hitoshi Sakakibara
  • Organizer: The Nitrate Transporter NRT2.5 Plays a Major Role in Nitrate Acquisition in Nitrogen-Starved Adult Arabidopsis. Nitrogen meeting
  • Place of Presentation: Puerto Varas ,Chile
  • Year and Date: 2013-11-20
• [Presentation] Setaria viridis as a model for C4 photosynthesis : full-length cDNAs and heavy ion beam mutants (2013)
  • Author(s): Takatoshi Kiba, Takuhiro Yoshida, Tomoko Abe, Tetsuya Sakurai, Hitoshi Sakakibara
  • Organizer: The International Symposium of C4 and CAM Plant Biology
  • Place of Presentation: Illinois, USA
  • Year and Date: 2013-08-08
• [Presentation] Root-borne trans-zeatin-type cytokinins can regulate shoot growth (2013)
  • Author(s): Takatoshi Kiba, Kentaro Takei, Mikiko Kojima and Hitoshi Sakakibara
  • Organizer: Plant Vascular Biology Mee ting
  • Place of Presentation: Helsinki, Finland
  • Year and Date: 2013-07-29
• [Presentation] The trans-zeatin-type cytokinin controls shoot growth in Arabidopsis (2013)
  • Author(s): Takatoshi Kiba, Kentaro Takei, Mikiko Kojima and Hitoshi Sakakibara
  • Organizer: International Conference on Plant Growth Substances
  • Place of Presentation: Shanghi, China
  • Year and Date: 2013-06-19
• [Presentation] iP型サイトカイニンとtZ型サイトカイニンの生理機能の違い (2013)
  • Author(s): 木羽隆敏,武井兼太郎,小嶋美紀子,榊原均
  • Organizer: 日本植物生理学会年会
  • Place of Presentation: 岡山
  • Year and Date: 2013-03-22
• [Presentation] Root-borne trans-zeatin-type cytokinins can regulate shoot growth (2013)
  • Author(s): Takatoshi Kiba, Kentaro Takei, Mikiko Kojima and Hitoshi Sakakibara
  • Organizer: Plant Vascular Biology Meeting
  • Place of Presentation: Helsinki, Finland
• [Presentation] Setaria viridis as a model for C4 photosynthesis: full-length cDNAs and heavy ion beam mutants (2013)
  • Author(s): Takatoshi Kiba, Takuhiro Yoshida, Tomoko Abe, Tetsuya Sakurai, Hitoshi Sakakibara
  • Organizer: International Symposium of C4 and CAM Plant Biology
  • Place of Presentation: Illinois, USA
• [Presentation] The Nitrate Transporter NRT2.5 Plays a Major Role in Nitrate Acquisition in Nitrogen-Starved Adult Arabidopsis (2013)
  • Author(s): Takatoshi Kiba, Lina Lezhneva, Ana Belen Feria-Bourellier, Florence Lafouge, Stephanie Boutet-Mercey, Nino Niccolo, Hitoshi Sakakibara, Francoise Daniel-Vedele, Anne Krapp
  • Organizer: Nitrogen meeting
  • Place of Presentation: Puerto Varas, Chile
• [Presentation] iP型サイトカイニンとtZ型サイトカイニンの生理機能の違い (2013)
  • Author(s): 木羽隆敏、武井兼太郎、小嶋美紀子、榊原均
  • Organizer: 第54回日本植物生理学会年会
  • Place of Presentation: 岡山大学
• [Presentation] The trans-zeatin-type cytokinin is indispensable for shoot growth regulation in Arabidopsis (2012)
  • Author(s): Takatoshi Kiba, Kentaro Takei, Mikiko Kojima, Hitoshi Sakakibara
  • Organizer: 2 ^<nd> Cytokinin Meeting
  • Place of Presentation: Berlin, Germany
  • Year and Date: 2012-07-09
• [Presentation] The trans-zeatin-type cytokinin is indispensable for shoot growth regulation in Arabidopsis (2012)
  • Author(s): Takatoshi Kiba, Kentaro Takei, Mikiko Kojima, Hitoshi Sakakibara
  • Organizer: International conference on Arabidopsis Research
  • Place of Presentation: Vienna, Austria
  • Year and Date: 2012-07-05
• [Presentation] THE TRANS-ZEATIN-TYPE CYTOKININ IS INDISPENSABLE FOR SHOOT GROWTH REGULATION IN ARABIDOPSIS (2012)
  • Author(s): Takatoshi Kiba, Kentaro Takei, Mikiko Kojima, Hitoshi Sakakibara
  • Organizer: 23rd International Conference on Arabidopsis Research
  • Place of Presentation: Vienna, Austria
• [Presentation] The trans-zeatin-type cytokinin is indispensable for shoot growth regulation in Arabidopsis (2012)
  • Author(s): Takatoshi Kiba, Kentaro Takei, Mikiko Kojima, Hitoshi Sakakibara
  • Organizer: 2nd meeting “Cytokinin metabolism, signaling and function”
  • Place of Presentation: Berlin, Germany
• [Remarks] ○ホームページ
  • URL: http://www.riken.jp/research/labs/csrs/plant_prod_sys/
• [Remarks] ①超低濃度の窒素栄養を効率よく吸収する仕組みをシロイヌナズナで解明
  • URL: http://www.riken.jp/pr/press/2012/20120111/
• [Remarks] ②植物ホルモン「サイトカイニン」の「質」の重要性を解明
  • URL: http://www.riken.jp/pr/press/2013/20131126_1/
• [Remarks] ○ライフサイエンス新着レビューサイトカイニンの作用の器官に対する特異性は側鎖の修飾により制御される
  • URL: http://first.lifesciencedb.jp/archives/8063
• [Remarks] プレスリリース：超低濃度の窒素栄養を効率よく吸収する仕組みをシロイヌナズナで解明
  • URL: http://www.riken.jp/pr/press/2012/20120111/
• [Remarks] プレスリリース：植物ホルモン「サイトカイニン」の「質」の重要性を解明
  • URL: http://www.riken.jp/pr/press/2013/20131126_1/
• [Remarks] ライフサイエンス新着レビュー：サイトカイニンの作用の器官に対する特異性は側鎖の修飾により制御され
  • URL: http://first.lifesciencedb.jp/archives/8063