Elucidation of signal transduction pathways for pellicle formation in wild yeast and its application to develop a novel method for preventing deleterious pellicle formation in wineries
Project/Area Number |
24780069
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Applied microbiology
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Research Institution | University of Yamanashi |
Principal Investigator |
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Project Period (FY) |
2012-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
|
Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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Keywords | 野生酵母 / 産膜 / ワイン / FLO11 / FLO8 / 遺伝子破壊 / Flo8p / グルコース / プロモーター置換 / グルコースシグナル伝達経路 |
Outline of Final Research Achievements |
In this study, we revealed that the glucose signal transduction pathway acting on FLO11 gene, which encodes a cell surface glycoprotein, is one of the signal transduction pathways for pellicle formation by a wild pellicle-forming strain of Saccharomyces cerevisiae isolated from contaminated wine in Yamanashi prefecture, Japan. We also found that the wild pellicle-forming strain had the deletion of the 111-bp repression sequence in the FLO11 promoter. Furthermore, we found that FLO8 gene encoding a transcriptional activator in the glucose signal transduction pathway is required for pellicle formation by the wild pellicle-forming strain. Based on the results obtained in this study, yeast or lactic acid bacteria strains which secrete inhibitors of Flo8p would be useful to develop a novel method for preventing deleterious pellicle formation in wineries.
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Report
(5 results)
Research Products
(2 results)