| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
Autoregulators elicit secondary metabolism by binding to the cognate receptors in actinomycetes. In this study, I report the molecular mechanism for the regulation of secondary metabolism by a butenolide-type autoregulator as another type of signaling molecule, the identification of actinomycetes producing the autoregulator, and the useful method for finding sleeping compounds in the genome. I demonstrated that the regulatory network composed of autoregulator, receptor, receptor-homologues, and autoregulator biosynthetic enzymes controls antibiotic production in Streptomyces avermitilis. Furthermore, gene inactivation of the receptor (homologue) genes or exogenous addition of the autoregulator led to increased production of silent compounds. These findings indicated that a butenolide-type autoregulator is a key molecule for not only understanding secondary metabolism in actinomycetes but also activating the numerous useful silent natural products.
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