Understanding the nitrogen assimilation by the mathematical model in Bacillus subtilis

• Project/Area Number: 24780072
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Applied microbiology
• Research Institution: Chiba University
• Principal Investigator: TAKAHASHI Hiroki 千葉大学, 真菌医学研究センター, 准教授 (60548460)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 枯草菌 / 窒素同化 / 数理モデル

Research Abstract

We performed the monitoring of the growth rate of Bacillus subtilis WT and MGB874 strains after adding the nitrogen source. As the results, there were not significant difference between the responses to nitrogen in WT and those in MGB874. Moreover, GeF-Seq analysis for sigA was conducted. We revealed 13 sigA biding sites only in MGB874 strain. Interestingly, sigA was bound to the upper region of glnR, suggesting that the accumulation of glutamic acid in MGB874 would be affected by alternation of sigA profile.

Research Products

• [Journal Article] Functions of the Hha and YdgT Proteins in Transcriptional Silencing by the Nucleoid Proteins, H-NS and StpA, in Escherichia coli. (2013)
  • Author(s): Ueda T, Takahashi H, Uyar E, Ishikawa S, Ogasawara N, Oshima T.
  • Journal Title: DNA Res. Volume: Epub ahead of print Issue: 3 Pages: 263-71
  • DOI: 10.1093/dnares/dst008
  • Peer Reviewed
• [Journal Article] Improved production of secreted heterologous enzyme in Bacillus subtilis strain MGB874 via modification of glutamate metabolism and growth conditions (2013)
  • Author(s): Manabe K, Kageyama Y, Morimoto T, Shimizu E, Takahashi H, Kanaya S, Ara K, Ozaki K, Ogasawara N.
  • Journal Title: Microbial Cell Factories Volume: 12 Issue: 1 Pages: 18-18
  • DOI: 10.1186/1475-2859-12-18
  • Peer Reviewed
• [Journal Article] 数理モデルに触れてみよう (2013)
  • Author(s): 高橋弘喜
  • Journal Title: 日本生物工学会.生物工学会誌 Volume: Vol91 Pages: 2-2
  • NAID: 110009597780
• [Journal Article] 数理モデルに触れてみよう (2013)
  • Author(s): 高橋弘喜
  • Journal Title: 生物工学会誌 Volume: Vol. 91 Pages: 109-109
  • NAID: 110009597780
• [Journal Article] Prediction of operon-like gene clusters in the Arabidopsis thaliana genome based on co-expression analysis of neighboring genes (2012)
  • Author(s): Wada M, Takahashi H, Altaf-Ul-Amin M, Nakamura K, Hirai MY, Ohta D, Kanaya S.
  • Journal Title: Gene Volume: 503(1) Issue: 1 Pages: 56-64
  • DOI: 10.1016/j.gene.2012.04.043
  • Peer Reviewed
• [Presentation] 数理モデルアプローチによる大腸菌の亜鉛制御機構の解明 (2013)
  • Author(s): 高橋弘喜,大島拓, Clayton Selina R, Hobman Jon L,戸邉亨,金谷重彦,小笠原直毅, Stekel Dov J.
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸
• [Presentation] 大腸菌Hha,YdgTタンパク質による外来性遺伝子の転写抑制 (2013)
  • Author(s): 上田剛士,高橋弘喜,石川周,小笠原直毅,大島拓
  • Organizer: 第7回日本ゲノム微生物学会
  • Place of Presentation: 滋賀
• [Presentation] 大腸菌Hha,YdgTタンパク質による外来性遺伝子の転写抑制 (2013)
  • Author(s): 上田剛士，高橋弘喜，石川周，小笠原直毅，大島拓
  • Organizer: 第7回日本ゲノム微生物学会
  • Place of Presentation: 長浜
• [Presentation] The role of zinc uptake regulator, Zur, in pathogenic and non-pathogenic Escherichia coli (2012)
  • Author(s): Clayton, S.R., Patel, M.D., Constantinidou, C., Oshima, T., Takahashi, H., Heurlier, K., Stekel, D.J., Hobman, J.L.
  • Organizer: Biometals 2012
  • Place of Presentation: Brussels, Belgium
• [Presentation] Mathematical modelling towards understanding zinc homeostasis in Escherichia coli (2012)
  • Author(s): Takahashi H, Oshima T, Clayton SR, Hobman JL, Tobe T, Kanaya S, Ogasawara N, Stekel DJ.
  • Organizer: Biometals 2012
  • Place of Presentation: Brussels, Belgium
• [Presentation] 大腸菌における亜鉛制御機構の数理モデルによる理解を目指して (2012)
  • Author(s): 高橋弘喜, 大島拓, Selina R. Clayton, Jon L. Hobman, 戸邉 亨, 金谷重彦, 小笠原直毅, Dov J. Stekel
  • Organizer: 第6回細菌学若手コロッセウム
  • Place of Presentation: 八王子
• [Presentation] The role of zinc uptake regulator, Zur, in pathogenic and non-pathogenic Escherichia coli. (2012)
  • Author(s): Clayton, S.R., Patel, M.D., Constantinidou, C., Oshima, T., Takahashi, H., Heurlier, K., Stekel, D.J., Hobman, J.L.
  • Organizer: Biometals 2012
  • Place of Presentation: Brussels, ベルギー
• [Presentation] MATHEMATICAL MODELLING TOWARDS UNDERSTANDING ZINC HOMEOSTASIS IN ESCHERICHIA COLI (2012)
  • Author(s): Takahashi H, Oshima T, Clayton SR, Hobman JL, Tobe T, Kanaya S, Ogasawara N, Stekel DJ.
  • Organizer: Biometals 2012
  • Place of Presentation: Brussels, ベルギー
• [Remarks]
  • URL: http://bioinfo.pf.chiba-u.jp/
• [Remarks] 論文発表
  • URL: http://ht02.pf.chiba-u.jp/?p=332
• [Remarks] 論文発表
  • URL: http://ht02.pf.chiba-u.jp/?p=284
• [Remarks] 生物工学会誌に掲載されました
  • URL: http://ht02.pf.chiba-u.jp/?p=264