| Budget Amount *help |
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
Cysteine desulfurase is a PLP-dependent enzyme and generates sulfur atom for the biosynthesis of sulfur-containing compounds, such as thiamine, biotin, and FeS cluster. We used L-[35S]cysteine as a tracer to identify proteins that accept sulfur atom for the biosynthesis of sulfur-containing cofactors in Bifidobacterium adorescentis. We have detected two 35S-labeled proteins by autoradiography. We also focused on the five genes located around cysteine desulfurase gene. Bad0711 and Bad0714 enhanced the cysteine desulfurase activativity and diminished the amount of 35S-label on cysteine desulfurases molecule, respectively. These results suggest that Bad0711 and Bad0714 promote the removal of sulfur on cysteine desulfurases.
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