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Anaerobic degradation pathway of hetero-aromatic compounds

Research Project

Project/Area Number 24780076
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Applied microbiology
Research InstitutionUniversity of Miyazaki

Principal Investigator

INOUE Kengo  宮崎大学, IR推進機構, 助教 (70581304)

Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywords鉄還元細菌 / 芳香族化合物 / ダイオキシン / 嫌気性細菌 / 環境汚染物質 / 分解微生物 / 環境浄化 / 分解系遺伝子
Research Abstract

Anaerobic biodegradation of heterocyclic compounds, carbazole, dibenzofuran, and dibenzothiophene were investigated. Bacterial strains capable of degrading the heterocyclic compounds were isolated from enriched culture in which the heterocyclic compounds and insoluble Fe(III) oxide were added as the sole electron donor and accepter, respectively. Inocula were collected from river and dam sediments and soils. Among the enriched cultures, 10, 1, and 1 strains were isolated from the medium in which carbazole, dibenzofuran, and dibenzothiophene were used as the sole electron donor, respectively. 16S rDNA sequencing analysis revealed one of the carbazole-degrading isolates belongs to Desulfovibrionaceae. Its type strain could not degrade carbazole and, thus, their comparative genome analysis is now underway in order to identify the gene involved in carbazole degradation.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (30 results)

All 2014 2013 2012 2011 Other

All Journal Article (7 results) (of which Peer Reviewed: 7 results,  Open Access: 2 results) Presentation (16 results) (of which Invited: 2 results) Book (5 results) Remarks (2 results)

  • [Journal Article] Structural Basis of the Divergent Oxygenation Reactions Catalyzed by the Rieske Nonheme Iron Oxygenase Carbazole 1,9a-Dioxygenase2014

    • Author(s)
      Kengo Inoue, Yusuke Usami, Yuji Ashikawa, Haruko Noguchi, Takashi Umeda, Aiko Yamagami-Ashikawa, Tadafumi Horisaki, Hiromasa Uchimura, Tohru Terada, Shugo Nakamura, Kentaro Shimizu, Hiroshi Habe, Hisakazu Yamane, Zui Fujimoto and Hideaki Nojiri
    • Journal Title

      Applied and Environmental Microbiology

      Volume: Volume 80 Issue: 9 Pages: 2821-2832

    • DOI

      10.1128/aem.04000-13

    • Related Report
      2013 Final Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Uptake of self-secreted flavins as bound cofactors for extracellular electron transfer in Geobacter species2014

    • Author(s)
      Akihiro Okamoto, Koichiro Saito, Kengo Inoue, Kenneth H. Nealson, Kazuhito Hashimoto, Ryuhei Nakamura
    • Journal Title

      Energy Environ. Sci.

      Volume: 7 Issue: 4 Pages: 1357-1361

    • DOI

      10.1039/c3ee43674h

    • Related Report
      2013 Final Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Electricity generation from cattle-manure slurries by cassette-electrode microbial fuel cells2013

    • Author(s)
      Inoue K, Ito T, Kawano Y, Iguchi A, Miyahara M, Suzuki Y, Watanabe K
    • Journal Title

      J. Biosci. Bioeng.

      Volume: 116 Issue: 5 Pages: 610-615

    • DOI

      10.1016/j.jbiosc.2013.05.011

    • NAID

      110009685213

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Structural insight into the substrate- and dioxygen-binding manner in the catalytic cycle of Rieske nonheme iron oxygen system, carbazole 1,9a-dioxygenase2013

    • Author(s)
      Ashikawa, Y., Fujimoto, Z., Usami, Y., Inoue, K.
    • Journal Title

      BMC Structure

      Volume: 12巻 Issue: 1 Pages: 15-15

    • DOI

      10.1186/1472-6807-12-15

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] 芳香族化合物酸素添加酵素の電子伝達システム2012

    • Author(s)
      井上謙吾,梅田隆志,松澤淳,野尻秀昭
    • Journal Title

      Journal of Environmental Biotechnology

      Volume: 12巻 Pages: 113-121

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identification of multi-component His-Asp phosphorelay system controlling flagella and motility gene expression in Geobacter species2012

    • Author(s)
      Ueki, T., Leang, C., Inoue, K., Lovley, D.R.
    • Journal Title

      Journal of Biological Chemistry

      Volume: 287巻 Issue: 14 Pages: 10958-10966

    • DOI

      10.1074/jbc.m112.345041

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] 芳香族化合物酸素添加酵素の電子伝達システム2012

    • Author(s)
      井上謙吾,梅田隆志,松澤淳,野尻秀昭
    • Journal Title

      Journal of Environmental Biotechnology

      Volume: 12 Pages: 113-121

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] 高い発電能力を有する新規Geobacter sulfurreducensの比較ゲノム解析2014

    • Author(s)
      河野好裕、小椋義俊、井上謙吾
    • Organizer
      日本農芸化学会
    • Place of Presentation
      東京(明治大学)
    • Year and Date
      2014-03-28
    • Related Report
      2013 Final Research Report
  • [Presentation] 微生物による鉄還元と発電~Geobacter属細菌の細胞外電子伝達機構~2013

    • Author(s)
      井上謙吾
    • Organizer
      日本微生物生態学会
    • Place of Presentation
      鹿児島(鹿児島大学)
    • Year and Date
      2013-11-23
    • Related Report
      2013 Final Research Report
  • [Presentation] Geobacter属細菌における細胞外電子伝達機構とその応用2013

    • Author(s)
      井上謙吾
    • Organizer
      日本生化学会
    • Place of Presentation
      横浜(パシフィコ横浜)
    • Year and Date
      2013-09-19
    • Related Report
      2013 Final Research Report
  • [Presentation] Electricity generation and treatment of water-suspended cattle manure by microbial fuel cells2013

    • Author(s)
      K. Inoue, T. Ito, Y. Kawano, A. Iguchi, M. Miyahara, Y. Suzuki, K. Watanabe
    • Organizer
      4th International Microbial Fuel Cell Conference
    • Place of Presentation
      Cairns (Australia)
    • Year and Date
      2013-09-01
    • Related Report
      2013 Final Research Report
  • [Presentation] Geobacter sulfurreducensにおけるOmcZの修飾と分泌に関わる遺伝子の解析2013

    • Author(s)
      井上謙吾,徳石崇宏,河野好裕,榊原陽一,水光正仁
    • Organizer
      日本環境バイオテクノロジー学会
    • Place of Presentation
      北九州
    • Year and Date
      2013-05-31
    • Related Report
      2013 Final Research Report
  • [Presentation] 微生物燃料電池で発電能力を有するGeobacter属細菌の単離2013

    • Author(s)
      河野好裕、小椋義俊、井上謙吾
    • Organizer
      日本農芸化学会
    • Place of Presentation
      仙台(東北大学)
    • Year and Date
      2013-03-25
    • Related Report
      2013 Final Research Report
  • [Presentation] 電子と生命,そしてバイオテクノロジー2013

    • Author(s)
      井上謙吾
    • Organizer
      日本生化学会大会
    • Place of Presentation
      パシフィコ横浜
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] 微生物の電子伝達による環境汚染物質分解と発電2012

    • Author(s)
      井上謙吾
    • Organizer
      極限環境生物学会
    • Place of Presentation
      東京(日本大学)
    • Year and Date
      2012-12-01
    • Related Report
      2013 Final Research Report
  • [Presentation] 廃棄物処理と発電を同時に行う微生物燃料電池2012

    • Author(s)
      井上謙吾
    • Organizer
      日本ゲノム微生物学会若手の会
    • Place of Presentation
      静岡
    • Year and Date
      2012-09-27
    • Related Report
      2013 Final Research Report
  • [Presentation] 電子伝達で環境浄化とバイオエネルギー2012

    • Author(s)
      井上謙吾
    • Organizer
      日本微生物生態学会若手の会
    • Place of Presentation
      豊橋(豊橋技術科学大学)
    • Year and Date
      2012-09-19
    • Related Report
      2013 Final Research Report
  • [Presentation] 微生物燃料電池を用いた牛糞尿からの発電と微生物群集構造解析2012

    • Author(s)
      伊藤寿宏,鈴木祥広,渡邉一哉,井上謙吾
    • Organizer
      第49回環境工学研究フォーラム
    • Place of Presentation
      京都
    • Related Report
      2013 Final Research Report
  • [Presentation] 微生物燃料電池の発電に関わる遺伝子に関する研究2012

    • Author(s)
      徳石崇宏,河野好裕,榊原陽一,水光正仁,井上謙吾
    • Organizer
      日本生物工学会
    • Place of Presentation
      神戸
    • Related Report
      2013 Final Research Report
  • [Presentation] 微生物燃料電池において高い発電能力を有する菌株の単離2012

    • Author(s)
      河野好裕,井上謙吾
    • Organizer
      日本生物工学会
    • Place of Presentation
      神戸
    • Related Report
      2013 Final Research Report
  • [Presentation] 微生物燃料電池での発電に関わるシトクロムcの機能と局在性2012

    • Author(s)
      井上謙吾
    • Organizer
      環境バイオテクノロジー学会
    • Place of Presentation
      京都大学
    • Related Report
      2012 Research-status Report
  • [Presentation] 微生物の電子伝達による環境汚染物質分解と発電2012

    • Author(s)
      井上謙吾
    • Organizer
      極限環境生物学会
    • Place of Presentation
      日本大学
    • Related Report
      2012 Research-status Report
  • [Presentation] 電子伝達で環境浄化とバイオエネルギー2012

    • Author(s)
      井上謙吾
    • Organizer
      日本微生物生態学会若手の会
    • Place of Presentation
      豊橋技術科学大学
    • Related Report
      2012 Research-status Report
    • Invited
  • [Book] Bioegradative Bacteria. Structure and function of aromatic-ring hydroxylating dioxygenase system, Nojiri, H., Tsuda, M., Fukuda, M., and Kamagata, Y., eds.2014

    • Author(s)
      井上謙吾
    • Total Pages
      358
    • Publisher
      エヌ・ティー・エス出版
    • Related Report
      2013 Final Research Report
  • [Book] 『微生物燃料電池による発電・省エネ型廃棄物・廃水処理技術最前線』「第2章牛糞を原料とする微生物燃料電池の開発」2013

    • Author(s)
      Inoue K, 他
    • Publisher
      Springer
    • Related Report
      2013 Final Research Report
  • [Book] Biodegradative bacteria2013

    • Author(s)
      Inoue, K., Nojiri, H.
    • Publisher
      Springer
    • Related Report
      2012 Research-status Report
  • [Book] 月刊バイオインダストリー2013

    • Author(s)
      井上謙吾
    • Total Pages
      6
    • Publisher
      シーエムシー出版
    • Related Report
      2012 Research-status Report
  • [Book] 『月刊バイオインダストリー』「電流生成菌の発電機構と発電に影響を与える因子」2011

    • Author(s)
      井上謙吾
    • Publisher
      シーエムシー出版
    • Related Report
      2013 Final Research Report
  • [Remarks]

    • URL

      http://www.cc.miyazaki-u.ac.jp/kinoue/top

    • Related Report
      2013 Final Research Report
  • [Remarks] http://www.cc.miyazaki-u.ac.jp/kinoue/top

    • Related Report
      2012 Research-status Report

URL: 

Published: 2013-05-31   Modified: 2019-07-29  

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