| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Research Abstract |
Anaerobic biodegradation of heterocyclic compounds, carbazole, dibenzofuran, and dibenzothiophene were investigated. Bacterial strains capable of degrading the heterocyclic compounds were isolated from enriched culture in which the heterocyclic compounds and insoluble Fe(III) oxide were added as the sole electron donor and accepter, respectively. Inocula were collected from river and dam sediments and soils. Among the enriched cultures, 10, 1, and 1 strains were isolated from the medium in which carbazole, dibenzofuran, and dibenzothiophene were used as the sole electron donor, respectively. 16S rDNA sequencing analysis revealed one of the carbazole-degrading isolates belongs to Desulfovibrionaceae. Its type strain could not degrade carbazole and, thus, their comparative genome analysis is now underway in order to identify the gene involved in carbazole degradation.
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