Anaerobic degradation pathway of hetero-aromatic compounds
Project/Area Number |
24780076
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Applied microbiology
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Research Institution | University of Miyazaki |
Principal Investigator |
INOUE Kengo 宮崎大学, IR推進機構, 助教 (70581304)
|
Project Period (FY) |
2012-04-01 – 2014-03-31
|
Project Status |
Completed (Fiscal Year 2013)
|
Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
|
Keywords | 鉄還元細菌 / 芳香族化合物 / ダイオキシン / 嫌気性細菌 / 環境汚染物質 / 分解微生物 / 環境浄化 / 分解系遺伝子 |
Research Abstract |
Anaerobic biodegradation of heterocyclic compounds, carbazole, dibenzofuran, and dibenzothiophene were investigated. Bacterial strains capable of degrading the heterocyclic compounds were isolated from enriched culture in which the heterocyclic compounds and insoluble Fe(III) oxide were added as the sole electron donor and accepter, respectively. Inocula were collected from river and dam sediments and soils. Among the enriched cultures, 10, 1, and 1 strains were isolated from the medium in which carbazole, dibenzofuran, and dibenzothiophene were used as the sole electron donor, respectively. 16S rDNA sequencing analysis revealed one of the carbazole-degrading isolates belongs to Desulfovibrionaceae. Its type strain could not degrade carbazole and, thus, their comparative genome analysis is now underway in order to identify the gene involved in carbazole degradation.
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Report
(3 results)
Research Products
(30 results)
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[Journal Article] Structural Basis of the Divergent Oxygenation Reactions Catalyzed by the Rieske Nonheme Iron Oxygenase Carbazole 1,9a-Dioxygenase2014
Author(s)
Kengo Inoue, Yusuke Usami, Yuji Ashikawa, Haruko Noguchi, Takashi Umeda, Aiko Yamagami-Ashikawa, Tadafumi Horisaki, Hiromasa Uchimura, Tohru Terada, Shugo Nakamura, Kentaro Shimizu, Hiroshi Habe, Hisakazu Yamane, Zui Fujimoto and Hideaki Nojiri
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Journal Title
Applied and Environmental Microbiology
Volume: Volume 80
Issue: 9
Pages: 2821-2832
DOI
Related Report
Peer Reviewed / Open Access
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