| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Research Abstract |
In order to elucidate the mechanism that Ideonella sp. No. 201-F6 strain grows on poly(ethylene terephthalate) (PET) as a carbon source, enzymes involved in the PET metabolism were explored. Whole genome analysis of this strain revealed the metabolic potential for various aromatic compounds containing terephthalic acid. Biochemical analyses for selected proteins through the genome/transcriptome information based screening identified two novel enzymes functioning as PET hydrolase and mono(2-hydroxyethyl) terephthalic acid hydrolase, respectively. These discoveries suggested the metabolic route from PET to carbon dioxide and water in this bacterium.
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