Novel extracellular function of molecular chaperones and mechanisms of biofilm formation in pathogenic bacteria
| Project/Area Number |
24780079
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied microbiology
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| Research Institution | Jikei University School of Medicine |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | バイオフィルム / 分子シャペロン / ClpB / DnaK / マトリクス / 黄色ブドウ球菌 / 抗体 / 阻害剤 / Myricetin / persister / アミロイド線維 / curli / 細胞外マトリクス / 病原細菌 / 線虫 / ブドウ球菌 |
|---|
| Outline of Final Research Achievements |
Molecular chaperones play key roles in protein quality control in the cell. We found that cytoplasmic molecular chaperones ClpB and DnaK are incorporated into extracellular matrixes of Staphylococcus aureus and are involved in biofilm development. We here report that extracellularly excreted ClpB promotes biofilm formation in an oligomerization- and disaggregation activity-independent manner. Inhibition of these molecular chaperones using antibodies or small compounds leads to prevention of biofilms and persisters. These findings provide a novel mechanistic insight into molecular chaperones as well as a therapeutic strategy for chronic infectious diseases.
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Report
(5 results)
Research Products
(40 results)
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[Journal Article] Immuno-Electron Microscopy of Primary Cell Cultures from GeneticallyModified Animals in Liquid by Atmospheric Scanning Electron Microscopy (ASEM)2014
Author(s)
T.Kinoshita, Y.Mori, K.Hirano, S.Sugimoto, K.Okuda, S.Matsumoto, T.Namiki, T.Ebihara, M.Kawata, H.Nishiyama, M.Sato, M.Suga, K.Higashiyama, K.Sonomoto, Y.Mizunoe, S.Nishihara, C.Sato
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Journal Title
Microscopy and Microanalysis
Volume: 20
Issue: 2
Pages: 469-483
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Presentation] Bacterial ATP secretion2013
Author(s)
Hironaka I, Iwase T, Sugimoto S, Okuda K, Tajima A, Yanaga K, Mizunoe Y
Organizer
Annual Conference of the Association for General and Applied Microbiology.
Place of Presentation
Bremen, Germany
Related Report
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