Resequencing analyses to identify the genes for high temperature tolerance.
| Project/Area Number |
24780082
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied microbiology
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| Research Institution | Tokyo University of Agriculture |
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Principal Investigator |
KANESAKI YU 東京農業大学, その他部局等, 博士研究員 (70380293)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | シアノバクテリア / 次世代シーケンサー / リシーケンス解析 / 突然変異 / 高温耐性 / 高温感受性 / 遊泳性 / 突然変異株 / 次世代シーケンス / リシーケンス / ゲノム / 高温ストレス |
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| Outline of Final Research Achievements |
Cyanobacteria are the photosynthetic microorganisms used as models for basic research on photosynthesis, evolution, and molecular genetics. Recent progress of sequencing technologies enables us to identify mutated loci in bacterial genomes. Here, we performed resequencing analysis of the genomes of five laboratory strains with different phenotypes on thermo-sensitivity. By verification of the identified mutations, we found a mutated locus which determined the thermo-sensitivity of the strains. Furthermore, we isolated the spontaneous thermo-tolerant mutants and identified a number of candidate loci for thermo-tolerance by resequencing analysis. Our strategy of genetics could efficiently identify the associated mutations in the spontaneous mutants which has altered phenotype.
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Report
(4 results)
Research Products
(18 results)
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[Journal Article] Genomic analysis of parallel-evolved cyanobacterium Synechocystis sp. PCC 6803 under acid stress.2015
Author(s)
Uchiyama J, Kanesaki Y, Iwata N, Asakura R, Funamizu K, Tasaki R, Agatsuma M, Tahara H, Matsuhashi A, Yoshikawa H, Ogawa S, Ohta H
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Journal Title
Photosynth Res
Volume: -
Issue: 1-2
Pages: 243-254
DOI
Related Report
Peer Reviewed
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[Journal Article] Analysis of spontaneous suppressor mutants from the photomixotrophically-grown pmgA-disrupted mutant in the cyanobacterium Synechocystis sp. PCC 68032015
Author(s)
Nishijima, Y., Kanesaki, Y., Yoshikawa, H., Ogawa, T., Sonoike, K., Nishiyama, Y. and Hihara, Y.
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Journal Title
Photosynth. Res.
Volume: -
Issue: 2-3
Pages: 465-475
DOI
Related Report
Peer Reviewed
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[Journal Article] Complete genome sequence of Bifidobacterium longum 105-A, a strain with high transformation efficiency.2014
Author(s)
Kanesaki, Y., Masutani, H., Sakanaka, M., Shiwa, Y., Fujisawa, T., Nakamura, Y., Yokota, A., Fukiya, S., Suzuki, T., Yoshikawa, H.
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Journal Title
Genome Announc.
Volume: 2
Issue: 6
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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