| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
Cyanobacteria are the photosynthetic microorganisms used as models for basic research on photosynthesis, evolution, and molecular genetics. Recent progress of sequencing technologies enables us to identify mutated loci in bacterial genomes. Here, we performed resequencing analysis of the genomes of five laboratory strains with different phenotypes on thermo-sensitivity. By verification of the identified mutations, we found a mutated locus which determined the thermo-sensitivity of the strains. Furthermore, we isolated the spontaneous thermo-tolerant mutants and identified a number of candidate loci for thermo-tolerance by resequencing analysis. Our strategy of genetics could efficiently identify the associated mutations in the spontaneous mutants which has altered phenotype.
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