| Budget Amount *help |
¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Research Abstract |
Bacterial binuclear iron monooxygenases play numerous physiological roles in oxidative metabolism. Monooxygenases of this type found in actinomycetes also catalyze various useful reactions and have attracted much attention as oxidation biocatalysts. However, difficulties in expressing these multicomponent monooxygenases in heterologous hosts have hampered the development of engineered oxidation biocatalysts. Here, we succeeded in functionally expressing the mycobacterial binuclear iron monooxygenase in heterologous hosts. We found that a specific chaperonin played essential roles in the active expression of the monooxygenase. The strategy developed here should be generally applicable to the heterologous expression of other actinomycetous binuclear iron monooxygenases, and will accelerate the development of engineered oxidation biocatalysts for industrial processes.
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