Functional analysis of alpha-1,3-glucanase: the enzyme participates in fungal cell-wall hydrolysis

• Project/Area Number: 24780085
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Applied microbiology
• Research Institution: Yamagata University
• Principal Investigator: YANO Shigekazu 山形大学, 理工学研究科, 助教 (50411228)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: α-1,3-グルカナーゼ / 細胞壁 / α-1,3-グルカン / 真菌細胞壁 / 糸状菌細胞壁

Research Abstract

Bacillus circulans KA-304 alpha-1,3-glucanase (Agl-KA) is a multi-domain enzyme, and it includes N-terminal DS1, CB6, DS2, UCD, and C-terminal catalytic domain. To clarify the role of these domains, we constructed several domain deletion enzymes and GFP-fusion proteins. As the results, DS1, CB6, and DS2 domain bound to alpha-1,3-glucan and fungal cell-wall, and that binding efficiency was increased by combined action. The findings indicate that DS1, CB6, and DS2 are novel alpha-1,3-glucan binding domain.
In order to obtain the further information on structure and function of alpha-1,3-glucanases, we purified and characterized a novel alpha-1,3-glucanase (Agl-FH1) of Paenibacillus glycanilyticus FH11. Although the properties of Agl-FH1 were almost the same as those of Agl-KA, the amino acid sequence of catalytic domain of Agl-FH1 showed approximately 20% identity to that of Agl-KA. The results may be beneficial to clarify the catalytic mechanism of the enzyme in the future.

Research Products

• [Journal Article] Characterization ofα-1,3-glucanase isozyme from Paenibacillus glycanilyticus FH11 in a new subgroup of family 87α-1,3-glucanase (2014)
  • Author(s): Suyotha W., Yano S., Itoh T., Fujimoto H., Hibi T., Tachiki T., Wakayama M.
  • Journal Title: J. Biosci. Bioeng. Volume: (in press)
  • Peer Reviewed
• [Journal Article] Characterization of α-1,3-glucanase isozyme from Paenibacillus glycanilyticus FH11 in a new subgroup of family 87 α-1,3-glucanase (2014)
  • Author(s): ワサナ スヨータ、矢野 成和、伊藤 貴文、藤本 寛子、日び 隆雄、立木 隆、若山 守
  • Journal Title: Journal of Bioscience and Bioengineering Volume: 117巻
  • NAID: 110009865418
  • Peer Reviewed
• [Journal Article] Functional analysis of theα-1,3-glucan synthase genes agsA and agsB in Aspergillus nidulans : agsB is the majorα-1,3-glucan synthase in this fungus (2013)
  • Author(s): Yoshimi A., Sano M., Inaba A., Kokubun Y., Fujioka T., Mizutani O., Hagiwara D., Fujikawa T., Nishimura M., Yano S., Kasahara S., Shimizu K., Yamaguchi M., Kawakami K., Abe K.
  • Journal Title: PLoS One Volume: 8(1)
  • Peer Reviewed
• [Journal Article] Domain structure and function ofα-1,3-glucanase from Bacillus circulans KA-304, an enzyme essential for degrading basidiomycete cell walls (2013)
  • Author(s): Suyotha W., Yano S., Takagi K., Rattanakit -Chandet N., Tachiki T., Wakayama M.
  • Journal Title: Biosci. Biotechnol. Biochem. Volume: 77(3) Pages: 639-647
  • Peer Reviewed
• [Journal Article] Domain Structure and Function of &#945;-1,3-Glucanase from <i>Bacillus circulans</i> KA-304, an Enzyme Essential for Degrading Basidiomycete Cell Walls (2013)
  • Author(s): ワサナ スヨータ、矢野 成和、高木 一好、ノパカーン ラタナキット-チャンデット、立木 隆、若山 守
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 77 Issue: 3 Pages: 639-647
  • DOI: 10.1271/bbb.120900
  • NAID: 10031164788
  • ISSN: 0916-8451, 1347-6947
  • Peer Reviewed
• [Journal Article] Surfaceα-1,3-glucan facilitates fungal stealth infection by interfering with innate immunity in plants (2012)
  • Author(s): Fujikawa T., Sakaguchi A., Nishizawa Y., Kouzai Y., Minami E., Yano S., Koga H., Meshi T., Nishimura M.
  • Journal Title: PLoS Pathog Volume: 8(8)
  • Peer Reviewed
• [Presentation] Bacillus circulans KA-304由来α-1,3-グルカナーゼの基質結合ドメイン (2013)
  • Author(s): 矢野成和、ワサナスヨータ、立木隆、若山守
  • Organizer: 日本農芸化学会東北支部第148回大会
  • Place of Presentation: 岩手大学農学部
  • Year and Date: 2013-10-26
• [Presentation] Paenibacillus sp. FH11が生成するα-1,3-グルカナーゼのクローニング (2013)
  • Author(s): ワサナスヨータ、藤本寛子、矢野成和、立木隆、若山守
  • Organizer: 第65回日本生物工学会大会
  • Place of Presentation: 広島国際会議場
  • Year and Date: 2013-09-19
• [Presentation] 細菌型α-1,3-グルカナーゼのドメイン構造 (2013)
  • Author(s): 矢野成和、ワサナスヨータ、立木隆、若山守
  • Organizer: 第65回日本生物工学会大会
  • Place of Presentation: 広島国際会議場
  • Year and Date: 2013-09-19
• [Presentation] 糸状菌細胞壁溶解に寄与するPaenibacilllus sp. No.11株由来α-1,3-グルカナーゼの解析 (2013)
  • Author(s): 矢野成和、ワサナスヨータ、藤本寛子、立木隆、若山守
  • Organizer: 日本農芸化学会2013年度大会
  • Place of Presentation: 東北大学
  • Year and Date: 2013-03-25
• [Presentation] Paenibacillus sp. FH11が生成する新規α-1,3-グルカナーゼのクローニング (2013)
  • Author(s): ワサナ スヨータ、、藤本 寛子、矢野 成和、立木 隆、若山 守
  • Organizer: 第65回日本生物工学会大会
  • Place of Presentation: 広島国際会議場
• [Presentation] 糸状菌細胞壁溶解に寄与するPaenibacillus sp. No. 11株由来α-1,3-グルカナーゼの解析 (2013)
  • Author(s): 矢野 成和、ワサナ スヨータ、藤本 寛子、立木 隆、若山 守
  • Organizer: 日本農芸化学会2013年度大会
  • Place of Presentation: 東北大学
• [Presentation] α-1,3-グルカナーゼ生産菌の探索と系統分類 (2012)
  • Author(s): 安井智美、ワサナスヨータ、矢野成和、立木隆、若山守
  • Organizer: 日本生物工学会第19回九州支部大分大会
  • Place of Presentation: 別府大学
  • Year and Date: 2012-12-01
• [Presentation] N-terminal region ofα-1,3-glucanase from Bacillus circulans KA-304 (2012)
  • Author(s): Suyotha W., Yano S., Tachiki T., Wakayama M.
  • Organizer: 15^<th> International Biotechnology Symposium and Exhibition
  • Place of Presentation: Exco. Daegu. Korea
• [Presentation] N-terminal region of alpha-1,3-glucanse from Bacillus circulans KA-304 (2012)
  • Author(s): ワサナ スヨータ，矢野 成和，立木 隆，若山 守
  • Organizer: 15th International Biotechnology Symposium and Exhibition.
  • Place of Presentation: Exco、Daegu, Korea.
• [Presentation] α-1,3-グルカナーゼ生産菌の探索と系統分類
  • Author(s): 安井 智美，ワサナ スヨータ，矢野 成和，立木 隆，若山 守
  • Organizer: 日本生物工学会 第19回九州支部大分大会
  • Place of Presentation: 別府大学