| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
The objective of this study is to find essential elements for the conjugative transfer of plasmid between different bacteria using pCAR1. Three kinds of nucleotide-associated proteins (NAPs) encoded on pCAR1 (Pmr, Phu, and Pnd) were shown to be important elements for its replication, maintenance and transfer in different host strains. Products of ORF145 and ORF146 on pCAR1 were not essential for the transfer but they may affect the transfer frequency of the plasmid. NAP genes were shown to be distributed on various transmissible plasmids. We successfully developed a method to detect, separate, and analyze ‘transient’ transconjugants, which would be important for the spread of plasmids in different host strains.
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