| Project/Area Number |
24780091
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Applied biochemistry
|
|---|
| Research Institution | Hokkaido University |
|---|
Principal Investigator |
SABURI Wataru 北海道大学, (連合)農学研究科(研究院), 助教 (00598089)
|
|---|
| Project Period (FY) |
2012-04-01 – 2014-03-31
|
| Project Status |
Completed (Fiscal Year 2013)
|
| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
|---|
| Keywords | マンノシルグルコースホスホリラーゼ / マンナン / 基質特異性 / Ruminococcus albus / 活性中心 / 触媒残基 / ホスホリラーゼ / オリゴ糖合成 / マンノオリゴ糖 / 加リン酸分解 |
|---|
| Research Abstract |
A ruminal anaerobic baceteium, Ruminococcus albus, has two mannosylglucose phosphorylase isozymes (Type-I and Type-II), phosphorolyzing mannosylglucose to alpha-mannose 1-phosphate and glucose. Anyalysis of substrate specificity revealed that Type-I was specific to mannosylglucose, but Type-II had higher activity to mannooligosaccharide than mannosylglucose. These enzymes showed different acceptor substrate specificity in the synthetic reaction: Type-I enzyme showed synthetic activity to 6-OH glucose derivatives unlike in contrast to Type-II, which was completely inert to these substrates. Asp129 of Type-I enzyme was determined to be catalytic amino acid residue based on sequence comparison and site-directed mutagenesis analysis. Ile212 of Type-I enzyme was important for recognition of 6-OH glucose derivatives as acceptor substrates in the synthetic reaction (reverse reaction).
|
