Molecular analysis of two novel carbohydrate phosphorylases
Project/Area Number |
24780091
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Applied biochemistry
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Research Institution | Hokkaido University |
Principal Investigator |
SABURI Wataru 北海道大学, (連合)農学研究科(研究院), 助教 (00598089)
|
Project Period (FY) |
2012-04-01 – 2014-03-31
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Project Status |
Completed (Fiscal Year 2013)
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Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
Keywords | マンノシルグルコースホスホリラーゼ / マンナン / 基質特異性 / Ruminococcus albus / 活性中心 / 触媒残基 / ホスホリラーゼ / オリゴ糖合成 / マンノオリゴ糖 / 加リン酸分解 |
Research Abstract |
A ruminal anaerobic baceteium, Ruminococcus albus, has two mannosylglucose phosphorylase isozymes (Type-I and Type-II), phosphorolyzing mannosylglucose to alpha-mannose 1-phosphate and glucose. Anyalysis of substrate specificity revealed that Type-I was specific to mannosylglucose, but Type-II had higher activity to mannooligosaccharide than mannosylglucose. These enzymes showed different acceptor substrate specificity in the synthetic reaction: Type-I enzyme showed synthetic activity to 6-OH glucose derivatives unlike in contrast to Type-II, which was completely inert to these substrates. Asp129 of Type-I enzyme was determined to be catalytic amino acid residue based on sequence comparison and site-directed mutagenesis analysis. Ile212 of Type-I enzyme was important for recognition of 6-OH glucose derivatives as acceptor substrates in the synthetic reaction (reverse reaction).
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Report
(3 results)
Research Products
(84 results)
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[Journal Article] Modulation of allosteric regulation by E38K and G101N mutations in the potate tuber ADP-glucose pyrophosphorylase2013
Author(s)
Shinji Wakuta, Yumi Shibata, Yumiko Yoshizaki, Wataru Saburi, Shigeki Hamada, Hiroyuki Ito, Seon-Kap Hwang, Thomas W. Okita, and Hirokazu Matsui
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Journal Title
Bioscience, Biotechnology, and Biochemistry
Volume: 77
Pages: 1854-1859
Related Report
Peer Reviewed
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[Journal Article] Metabolic mechanism of mannan in a ruminal bacterium, Ruminococcus albus, involving two mannoside phosphorylases and cellobiose 2-epimerase: discovery of a new carbohydrate phosphorylase, β-1,4-mannooligosaccharide phosphorylase2012
Author(s)
Ryosuke Kawahara, Wataru Saburi, Rei Odaka, Hidenori Taguchi, Shigeaki Ito, Haruhide Mori, and Hirokazu Matsui
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Journal Title
Journal of Biological Chemistry
Volume: 287
Issue: 50
Pages: 42389-42399
DOI
NAID
Related Report
Peer Reviewed
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[Journal Article] urification and characterization of a liuefying α-amylase from alkalophilic termophilic Bacillus sp. AAH-312012
Author(s)
Daehoon Kim, Naoki Morimoto, Wataru Saburi, Atsushi Mukai, Koji Imoto, Toshihiko Takehana, Seiji Koike, Haruhide Mori, Hirokazu Matsui
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Journal Title
Bioscience, Biotechnology, and Biochemistry
Volume: 76
Pages: 1378-1383
Related Report
Peer Reviewed
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