| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
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| Outline of Final Research Achievements |
Proteins are altered their structure by phosphorylation, and their functions are changed. Thus, the phosphorylation is key mechanism that involved in regulation of physiological functions. However, relationships between structural-switch and functional-switch by phosphorylation are still unclear. Elucidations of these relationships are expected to facilitate to elucidate mechanisms of phosphrylation-related diseases including cancer. We employed the structural switch of Pin1, that binds to phosphorylated proteins, to develop a novel detection system of phosphorylation-induced structural switch. Characterizations of the Pin1-dirived sensor protein indicated that this sensor protein can detect structural switch of Pin1 induced by phosphorylated sequence.
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