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Visualization of structure and function change of phosphorylatad protein.

Research Project

Project/Area Number 24780092
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Applied biochemistry
Research InstitutionTohoku University

Principal Investigator

HIDAKA Masafumi  東北大学, (連合)農学研究科(研究院), 助教 (00584848)

Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Keywordsタンパク質工学
Outline of Final Research Achievements

Proteins are altered their structure by phosphorylation, and their functions are changed. Thus, the phosphorylation is key mechanism that involved in regulation of physiological functions. However, relationships between structural-switch and functional-switch by phosphorylation are still unclear. Elucidations of these relationships are expected to facilitate to elucidate mechanisms of phosphrylation-related diseases including cancer. We employed the structural switch of Pin1, that binds to phosphorylated proteins, to develop a novel detection system of phosphorylation-induced structural switch. Characterizations of the Pin1-dirived sensor protein indicated that this sensor protein can detect structural switch of Pin1 induced by phosphorylated sequence.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (4 results)

All 2014 2013 2012

All Presentation (4 results)

  • [Presentation] Improvement of FRET based indicator for structural change of Pin12014

    • Author(s)
      岡部恵美子、日高將文、内田隆史
    • Organizer
      日本分子生物学会
    • Place of Presentation
      神奈川県横浜市・パシフィコ横浜
    • Year and Date
      2014-11-25 – 2014-11-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] FRET法を用いたPin1構造変化の可視化2014

    • Author(s)
      岡部恵美子、日高將文、内田隆史
    • Organizer
      日本農芸化学会2014年度大会
    • Place of Presentation
      神奈川県川崎市・明治大学
    • Related Report
      2013 Research-status Report
  • [Presentation] Visualization of Pin1 binding with c-Myc peptide by FRET-based indicators.2013

    • Author(s)
      岡部恵美子、日高將文、内田隆史
    • Organizer
      日本農芸化学会
    • Place of Presentation
      仙台
    • Related Report
      2012 Research-status Report
  • [Presentation] Visualization of Pin1-phosphopeptide assiciation with FRET-based indicators.2012

    • Author(s)
      岡部恵美子、日高將文、内田隆史
    • Organizer
      日本分子生物学会
    • Place of Presentation
      福岡
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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