Study of Y chromosome gene in sex differences of skeletal growth

• Project/Area Number: 24780094
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Applied biochemistry
• Research Institution: Ehime University
• Principal Investigator: KAZUKI INOUE 愛媛大学, 総合科学研究支援センター, 助教 (60623725)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000); Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: Y染色体 / 性差

Research Abstract

Males have higher bone mass and larger skeletal size than females. Such gender-specific bone phenotype has considered to be generated by the actions of sex steroid hormones. However, an uncharacterized Y chromosome gene is assumed as a significant factor to form gender-specific bone phenotype from clinical observations of patients mutated in the Growth Controlling region on the Y chromosome. To reveal the biological functions of Y chromosome gene in skeletal systems, we generated Y chromosome gene knockout mice by using a novel method. We revealed that Y chromosome gene regulates male skeletal length by controlling cartilage development. Taken together, the present study has uncovered a molecular basis of gender-specific cartilage development.

Research Products

• [Journal Article] Identification of Novel Transcription Factors in Osteoclast Differentiation using Genome–wide Analysis of Open Chromatin Determined by DNase-seq (2014)
  • Author(s): Inoue K and Imai Y
  • Journal Title: J Bone Miner Res Volume: (Epub ahead of print) Issue: 8 Pages: 1823-1832
  • DOI: 10.1002/jbmr.2229
  • Peer Reviewed
• [Journal Article] Estrogen receptor a in osteocytes regulates trabecular bone fo rmation in female mice. (2014)
  • Author(s): Kondoh S, Inoue K, Igaras hi K, Sugizaki H, Shirode-Fukuda Y, Inoue E, Yu T, Takeuchi JK, Kanno J, Bonewald LF, Imai Y.
  • Journal Title: Bone Volume: 60 Pages: 68-77
  • DOI: 10.1016/j.bone.2013.12.005
  • Peer Reviewed
• [Journal Article] Novel insights into histone modifiers in Adipogenesis (2013)
  • Author(s): Okuno Y, Inoue K, Imai Y
  • Journal Title: Adipocyte Volume: 2(4) Issue: 4 Pages: 285-8
  • DOI: 10.4161/adip.25731
  • Peer Reviewed
• [Journal Article] Autoimmune regulator, Aire, is a novel regulator of chondrocyte differentiation (2013)
  • Author(s): Si Y, Inoue K, Igarashi K, Kanno J, Imai Y
  • Journal Title: Biochem Biophys Res Commun Volume: 437(4) Issue: 4 Pages: 579-84
  • DOI: 10.1016/j.bbrc.2013.07.001
  • Peer Reviewed
• [Journal Article] Female sex hormones ameliorate arthritis in SKG mice (2013)
  • Author(s): Inoue K, Inoue E, Imai Y
  • Journal Title: Biochem Biophys Res Commun Volume: 434(4) Issue: 4 Pages: 740-5
  • DOI: 10.1016/j.bbrc.2013.03.111
  • Peer Reviewed
• [Journal Article] Vitamin D receptor in osteoblasts is a negative regulator of bone mass control (2013)
  • Author(s): Yamamoto Y, Yoshizawa T, Fukuda T, Shirode-Fukuda Y, Yu T, Sekine K, Sato T, Kawano H, Aihara K, Naka michi Y, Watanabe T, Shindo M, Inoue K, Inoue E, Tsuji N, Hoshino M, Karsenty G, Metzger D, Chambon P, Kato S, Imai Y.
  • Journal Title: Endocrinology. Volume: 154 Issue: 3 Pages: 1008-20
  • DOI: 10.1210/en.2012-1542
  • Peer Reviewed
• [Journal Article] Nuclear Receptors in Bone Physiology and Diseases (2013)
  • Author(s): Imai Y, Youn MY, Inoue K, Takada I, Kouzmenko A, Kato S
  • Journal Title: Physiol Rev Volume: 93(2) Issue: 2 Pages: 481-523
  • DOI: 10.1152/physrev.00008.2012
  • Peer Reviewed
• [Journal Article] Nuclear Receptors in Bone Physiology and Diseases (2012)
  • Author(s): Imai Y, Youn MY, Inoue K, Takada I, Kouzmenko A, Kato S
  • Journal Title: Physiol Rev Volume: 93(2) Pages: 481-523
  • Peer Reviewed
• [Presentation] Identification of Novel Transcription Factors in Osteoclast differentiation using DNase-seq (2014)
  • Author(s): Kazuki Inoue and Yuuki Imai
  • Organizer: Herbert Fleisch Workshop
  • Place of Presentation: Brugge, Belgium
• [Presentation] Identification of Novel Transcription Factors in Osteoclast differentiation using DNase-seq (2014)
  • Author(s): Kazuki Inoue and Yuuki Imai
  • Organizer: Herbert Fleisch Workshop
  • Place of Presentation: Brugge (Belgium)
• [Presentation] Dynamic changes in chromatin accessibility during early osteoclastogenesin (2013)
  • Author(s): Kazuki Inoue and Yuuki Imai
  • Organizer: 2^<nd> Joint Meeting of the International Bone and Mineral Society and the Japan Society for Bone and Mineral Research
  • Place of Presentation: Kobe, Japan
• [Presentation] Dynamic changes in chromatin accessibility during early osteoclastogenesis (2013)
  • Author(s): Kazuki Inoue and Yuuki Imai
  • Organizer: 2nd Joint Meeting of the International Bone and Mineral Society and the Japanese Society for Bone and Mineral Research.
  • Place of Presentation: 神戸
• [Presentation] Dynamic changes in chromatin accessibility during early osteoclastogenesin (2012)
  • Author(s): Kazuki Inoue and Yuuki Imai
  • Organizer: ASBMR 2012 Annual Meeting
  • Place of Presentation: Minneapolis, USA
  • Year and Date: 2012-10-13
• [Presentation] Dynamic Changes of Chromatin Accessibility During Early Osteoclastogenesis (2012)
  • Author(s): KAZUKI INOUE, YUUKI IMAI
  • Organizer: ASBMR 2012 Annual Meeting
  • Place of Presentation: Minneapolis, USA