• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Functional analysis of human ABC transporters involved in HDL generation.

Research Project

Project/Area Number 24780101
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Applied biochemistry
Research InstitutionKyoto University

Principal Investigator

KIMURA Yasuhisa  京都大学, (連合)農学研究科(研究院), 助教 (10415143)

Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
KeywordsABCタンパク質 / ABCG1 / コレステロール / HDL / 膜輸送体 / タンパク質精製 / 高密度リポタンパク質 / 能動輸送 / 基質特異性 / ABCG4 / 脂質恒常性 / 高脂血症
Research Abstract

Cholesterol is an essential component for our body as a constituent of plasma membrane and precursor of steroid hormones. Since the excess cholesterol is harmful to cells, cholesterol homeostasis in peripheral tissues are tightly regulated. In this research, we purified human ABCG1 and ABCG4, and analyzed its substrate specificity by measuring ATPase activity.
Purified ABCG1 showed the ATPase activity that comparable to those of active transporters. The ATPase activity of ABCG1, reconstituted in phosphatidylserine liposome, was stimulated by cholesterol and choline phospholipids (especially sphingomyelin). The ATPase activity of ABCG4 was stimulated in cholesterol containing liposomes. Unlike ABCG1, choline phospholipids showed no effects on the ATPase activity. Our results suggest that ABCG1 and ABCG4 are active lipid transporters and ABCG1 recognizes cholesterol and sphingomyelin as transport substrates, while ABCG4 is a cholesterol specific transporter.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (20 results)

All 2014 2013 2012

All Journal Article (3 results) (of which Peer Reviewed: 2 results) Presentation (17 results)

  • [Journal Article] 脂質輸送型ABCタンパク質の基質認識2014

    • Author(s)
      木村泰久
    • Journal Title

      輸送機構

      Volume: 39(2) Pages: 68-73

    • Related Report
      2013 Final Research Report
  • [Journal Article] ATPase activity of human ABCG1 is stimulated by cholesterol and sphingomyelin2013

    • Author(s)
      Hirayama H, Kimura Y, Kioka N, Matsuo M, Ueda K
    • Journal Title

      J. Lipid Res.

      Volume: 54 Pages: 496-502

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] ATPase activity of human ABCG1 is stimulated by cholesterol and sphingomyelin.2013

    • Author(s)
      Hirayama, H., Kimura, Y., Kioka, N., Matsuo,M. and Ueda, K.
    • Journal Title

      J. Lipid Res.

      Volume: 54 Issue: 2 Pages: 496-502

    • DOI

      10.1194/jlr.m033209

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] 精製タンパク質を用いたヒトABCタンパク質の機能解析2014

    • Author(s)
      木村泰久
    • Organizer
      日本農芸化学会2014年度大会
    • Place of Presentation
      東京
    • Year and Date
      2014-03-30
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] Substrate recognition of human ABCG1 and ABCG42014

    • Author(s)
      Yasuhisa Kimura
    • Organizer
      5th FEBS special meeting,ATP-Binding Cassette (ABC) Proteins : From Multidrug Resistance to Genetic Diseases
    • Place of Presentation
      Innsbruck, Austria
    • Related Report
      2013 Final Research Report
  • [Presentation] Substrate recognition of human ABCG1 and ABCG42014

    • Author(s)
      Yasuhisa Kimura, Hiroshi Hirayama, Noriyuki Kioka, Michinori Matsuo, Kazumitsu Ueda
    • Organizer
      5th FEBS special meeting “ATP-Binding Cassette (ABC) Proteins: From Multidrug Resistance to Genetic Diseases”
    • Place of Presentation
      Innsbruck, Austria
    • Related Report
      2013 Annual Research Report
  • [Presentation] Characterization of the substrate specificity of ABCB62013

    • Author(s)
      Hajime Ohnuki
    • Organizer
      The 14th International Membrane Research Forum
    • Place of Presentation
      京都
    • Related Report
      2013 Final Research Report
  • [Presentation] Analysis of the substrate specificity of ABCB62013

    • Author(s)
      Hajime Ohnuki
    • Organizer
      RSC-iCeMSシンポジウム
    • Place of Presentation
      京都
    • Related Report
      2013 Final Research Report
  • [Presentation] Purification and functional analysis of cholesterol transporter ABCG12013

    • Author(s)
      Hiroshi Hirayama
    • Organizer
      RSC-iCeMSシンポジウム
    • Place of Presentation
      京都
    • Related Report
      2013 Final Research Report
  • [Presentation] 精製タンパク質を用いたヒトABCB6の機能解析2013

    • Author(s)
      大貫元
    • Organizer
      日本農芸化学会2013年度大会
    • Place of Presentation
      仙台
    • Related Report
      2013 Final Research Report 2012 Research-status Report
  • [Presentation] ATPase activity of human ABCG1 is stimulated by cholesterol and choline phospholipids2013

    • Author(s)
      Yasuhisa Kimura
    • Organizer
      Benzon Symposium No. 59 - Membrane proteins : Structure, Function and Dynamics
    • Place of Presentation
      Copenhagen, Denmark
    • Related Report
      2013 Final Research Report
  • [Presentation] ATPase activity of human ABCG1 is stimulated by cholesterol and choline phospholipids2013

    • Author(s)
      Yasuhisa Kimura, Hiroshi Hirayama, Noriyuki Kioka, Michinori Matsuo, Kazumitsu Ueda
    • Organizer
      Benzon Symposium No. 59 - Membrane proteins: Structure, Function and Dynamics
    • Place of Presentation
      Copenhagen, Denmark
    • Related Report
      2013 Annual Research Report
  • [Presentation] Characterization of the substrate specificity of ABCB62013

    • Author(s)
      Hajime Ohnuki, Yasuhisa Kimura, Michinori Matsuo, Noriyuki Kioka, Kazumitsu Ueda
    • Organizer
      The 14th International Membrane Research Forum
    • Place of Presentation
      Kyoto
    • Related Report
      2012 Research-status Report
  • [Presentation] Analysis of the substrate specificity of ABCB62013

    • Author(s)
      Hajime Ohnuki, Yasuhisa Kimura, Michinori Matsuo, Noriyuki Kioka, Kazumitsu Ueda
    • Organizer
      RSC-iCeMSシンポジウム
    • Place of Presentation
      Kyoto
    • Related Report
      2012 Research-status Report
  • [Presentation] Purification and functional analysis of cholesterol transporter ABCG12013

    • Author(s)
      Hiroshi Hirayama, Yasuhisa Kimura, Noriyuki Kioka, Michinori Matsuo, Kazumitsu Ueda
    • Organizer
      RSC-iCeMSシンポジウム
    • Place of Presentation
      Kyoto
    • Related Report
      2012 Research-status Report
  • [Presentation] ヒトABCB6の精製と機能解析2012

    • Author(s)
      大貫元
    • Organizer
      第85回日本生化学会大会
    • Place of Presentation
      福岡
    • Year and Date
      2012-12-15
    • Related Report
      2013 Final Research Report 2012 Research-status Report
  • [Presentation] K_ATPチャネル複合体のサブユニット間相互作用部位の探索2012

    • Author(s)
      井上修一
    • Organizer
      第85回日本生化学会大会
    • Place of Presentation
      福岡
    • Year and Date
      2012-12-15
    • Related Report
      2013 Final Research Report
  • [Presentation] Functional analysis of purified ABCG1 expressed in human cultured cells2012

    • Author(s)
      Hiroshi Hirayama
    • Organizer
      ASBMB : Frontiers in Lipid Biology
    • Place of Presentation
      Banff Canada
    • Related Report
      2013 Final Research Report
  • [Presentation] Functional analysis of purified ABCG1 expressed in human cultured cells2012

    • Author(s)
      Hiroshi Hirayama, Yasuhisa Kimura, Michinori Matsuo, Noriyuki Kioka, Kazumitsu Ueda
    • Organizer
      ASBMB: Frontiers in Lipid Biology
    • Place of Presentation
      Canada
    • Related Report
      2012 Research-status Report
  • [Presentation] KATPチャネル複合体のサブユニット間相互作用部位の探索2012

    • Author(s)
      井上修一、木村泰久、木岡紀幸、松尾道憲、植田和光
    • Organizer
      第85回日本生化学会大会
    • Place of Presentation
      福岡
    • Related Report
      2012 Research-status Report

URL: 

Published: 2013-05-31   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi