| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Research Abstract |
Peptides are attractive for novel biologically active substances. In this study, we attempted construction of a peptide library by chemo-enzymatic synthesis along with generation of bio-catalyst for synthesis of various peptides based on peptidases that catalyze peptide bond formation reaction as a side reaction. We exhaustively selected materials (substrates) for construction of peptide library using peptidases that exhibit peptide bond formation activity. By using the information, we constructed 900 kinds of enzyme reaction mixture library using the reaction of above enzymes. In addition, we found that the change in structure of active site of above enzymes by genetic engineering techniques exhibited increase of effectiveness for peptide bond formation.
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