| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
|---|
| Research Abstract |
L-Threonine dehydrogenase (ThrDH) gene from the pathogenic parasite Trypanosoma brucei was amplified using the overlapping primers, and ligated with the expression vector pCold TF. Escherichia coli was then transformed with the vector and induced by adding 1.0 mM IPTG to the medium. The gene was overexpressed in E. coli, and its product was purified and characterized. SDS-PAGE showed the subunit molecular mass of T. brucei ThrDH to be about 81 kDa, which was consistent with the molecular weight calculated from the amino acid sequence. The slightly NAD-dependent ThrDH activity was determined.
|
