33S solid-state NMR analysis of methionine radical inducing cytotoxicity of amyloid-beta protein

• Project/Area Number: 24780108
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Bioproduction chemistry/Bioorganic chemistry
• Research Institution: Tohoku University
• Principal Investigator: MASUDA YUICHI 東北大学, 薬学研究科(研究院), 助教 (90617755)
• Co-Investigator(Renkei-kenkyūsha): TAKEGOSHI Kiyonori 京都大学, 大学院理学研究科, 教授 (10206964) ; MURAKAMI Kazuma 京都大学, 大学院農学研究科, 准教授 (80571281)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 有機化学 / アミロイド / 固体NMR / 硫黄 / ラジカル

Research Abstract

We labeled sulfur atoms of methionine, cysteine and amyloid-beta with 33S by organic synthesis. We detected 33S NMR signals of methionine in solid-state and methionine sulfoxide in solution.

Research Products

• [Journal Article] Total Synthesis and Conformational Analysis of Apratoxin C (2014)
  • Author(s): Yuichi Masuda, Jun Suzuki, Masahito Yoshida, and Takayuki Doi
  • Journal Title: Peptide Science 2013 Volume: 巻番号なし Pages: 61-62
  • Peer Reviewed
• [Journal Article] Synthesis of Photoaffinity Probe of Beauveriolides for Binding-site Analysis (2014)
  • Author(s): Yuichi Masuda, Kazumasa Aoyama, Masahito Yoshida, Keisuke Kobayashi, Nobuhiro Koyama, Hiroshi Tomoda, and Takayuki Doi
  • Journal Title: Peptide Science 2013 Volume: 巻番号なし Pages: 149-150
  • Peer Reviewed
• [Journal Article] Exploring various modulation-sideband recoupling conditions of SHA+ sequence at fast MAS. (2013)
  • Author(s): Shen, M., Liu, Q., Trebosc, J., Lafon, O., Masuda, Y., Takegoshi, K., Amoureux, J. P., Hu, B., Chen, Q.
  • Journal Title: Solid State Nucl. Magn. Reson. Volume: 55-56 Pages: 42-47
  • DOI: 10.1016/j.ssnmr.2013.07.001
  • Peer Reviewed
• [Journal Article] Site-specific inhibitory mechanism for amyloid-β42 aggregation by catechol-type flavonoids targeting the Lys-residues (2013)
  • Author(s): Sato, M., Murakami, K., Ikubo, H., Uno, M., Nakagawa, Y., Katayama, S., Akagi, K.-i., Masuda, Y., Takegoshi, K., *Irie, K
  • Journal Title: J.Biol.Chem Volume: 288 Issue: 32 Pages: 23212-23224
  • DOI: 10.1074/jbc.m113.464222
  • NAID: 120005477416
  • Peer Reviewed
• [Journal Article] Solid-state NMR analysis of the beta-strand orientation of the protofibrils of amyloid beta-protein (2012)
  • Author(s): Takashi Doi
  • Journal Title: Biochem. Biophys. Res. Commun. Volume: 428 Issue: 4 Pages: 458-462
  • DOI: 10.1016/j.bbrc.2012.10.096
  • Peer Reviewed
• [Journal Article] Very-long-distance correlations in proteins revealed by solid-state NMR spectroscopy (2012)
  • Author(s): Hu, B., Trebosc, J., Lafon, O., Chen, Q., Masuda, Y., Takegoshi, K., Amoureux, J. P.
  • Journal Title: ChemPhysChem Volume: 13 Issue: 16 Pages: 3585-3588
  • DOI: 10.1002/cphc.201200548
  • Peer Reviewed
• [Journal Article] Lack of the Consensus Sequence Necessary for Tryptophan Prenylation in the ComX Pheromone Precursor (2012)
  • Author(s): F. Tsuji, A. Ishihara, A. Nakagawa, M. Okada, S. Kitamura, K. Kanamaru, Y. Masuda, K. Murakami, K. Irie, and Y. Sakagami.
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 76 Issue: 8 Pages: 1492-1496
  • DOI: 10.1271/bbb.120206
  • NAID: 10031065914
  • ISSN: 0916-8451, 1347-6947
  • Peer Reviewed
• [Presentation] アプラトキシンCの活性コンホメーションに着目した構造活性相関研究 (2014)
  • Author(s): 増田裕一，鈴木淳，吉田将人，土井隆行
  • Organizer: 日本薬学会第134年会
  • Place of Presentation: 熊本大学黒髪キャンパス，熊本
• [Presentation] 生物活性環状ペプチドPF1171類の固相法を用いた全合成 (2014)
  • Author(s): 増田裕一，田中錬，甲斐建次，A. Ganesan，土井隆行
  • Organizer: 日本薬学会第134年会
  • Place of Presentation: 熊本大学黒髪キャンパス，熊本
• [Presentation] タンパク質の高感度^<33>S-NMR解析に向けた^<33>S標識アミノ酸の合成 (2013)
  • Author(s): 増田裕一,松尾真嗣,水野敬,野田泰斗,竹腰清乃理,土井隆行
  • Organizer: 日本薬学会第133年会
  • Place of Presentation: 横浜(口頭発表, 講演番号29N-am09)
  • Year and Date: 2013-03-29
• [Presentation] ^<33>S標識によるタンパク質の高感度33S-NMR解析に向けて (2013)
  • Author(s): 増田裕一,松尾真嗣,水野敬,野田泰斗,竹腰清乃理,土井隆行
  • Organizer: 日本農芸化学会2013年度大会
  • Place of Presentation: 仙台(口頭発表, 講演番号4C26a03)
  • Year and Date: 2013-03-27
• [Presentation] Synthesis of Photoaffinity-Labeled Beauveriolides for Binding-Site Analysis (2013)
  • Author(s): Yuichi Masuda, Kazumasa Aoyama, Masahito Yoshida, Keisuke Kobayashi, Nobuhiro Koyama, Hiroshi Tomoda, and Takayuki Doi
  • Organizer: International Symposium for the 70th Anniversary of the Tohoku Branch of the Chemical Society of Japan
  • Place of Presentation: Tohoku Univ, Sendai, Japan
• [Presentation] タンパク質間相互作用阻害機構の解明に向けたスピンラベル化Thielocin B1 の合成研究 (2013)
  • Author(s): 大澤宏祐，吉田将人，増田裕一，土井隆行
  • Organizer: 第52回日本薬学会東北支部大会
  • Place of Presentation: 東北大学，仙台
• [Presentation] 結合部位解明を指向した光反応性官能基を有するBeauveriolide誘導体の合成研究 (2013)
  • Author(s): 増田裕一，青山和賢，吉田将人，小林啓介、小山信裕，供田洋，土井隆行
  • Organizer: 第52回日本薬学会東北支部大会
  • Place of Presentation: 東北大学，仙台
• [Presentation] Total Synthesis and Conformational Analysis of Apratoxin C (2013)
  • Author(s): Yuichi Masuda, Jun Suzuki, Masahito Yoshida, and Takayuki Doi
  • Organizer: APIPS 2013: 4th Asia-Pacific International Peptide Symposium, 50th Japanese Peptide Symposium
  • Place of Presentation: Hotel Hankyu Expo Park, Osaka
• [Presentation] Synthesis of Photoaffinity Probe of Beauveriolides for Binding-site Analysis (2013)
  • Author(s): Yuichi Masuda, Kazumasa Aoyama, Masahito Yoshida, Keisuke Kobayashi, Nobuhiro Koyama, Hiroshi Tomoda, and Takayuki Doi
  • Organizer: APIPS 2013: 4th Asia-Pacific International Peptide Symposium, 50th Japanese Peptide Symposium
  • Place of Presentation: Hotel Hankyu Expo Park, Osaka
• [Presentation] 33S標識によるタンパク質の高感度33S-NMR解析に向けて (2013)
  • Author(s): 増田裕一，松尾真嗣，水野敬，野田泰斗，竹腰清乃理，土井隆行
  • Organizer: 日本農芸化学会2013年度大会
  • Place of Presentation: 東北大学
• [Presentation] タンパク質の高感度33S-NMR解析に向けた33S標識アミノ酸の合成 (2013)
  • Author(s): 増田裕一，松尾真嗣，水野敬，野田泰斗，竹腰清乃理，土井隆行
  • Organizer: 日本薬学会第133年会，
  • Place of Presentation: パシフィコ横浜
• [Presentation] Solid-state NMR analysis of interaction sites of curcumin and amyloidβ-protein fibrils (2012)
  • Author(s): Masuda, Y., Fukuchi, M., Yatagawa, T., Tada, M., Takeda, K., Irie, K., Akagi, K., Monobe, Y., Imazawa, Y., Takegoshi, K.
  • Organizer: IKCOC-12
  • Place of Presentation: Kyoto(口頭発表, Program Number OP-21)
  • Year and Date: 2012-11-14
• [Presentation] Specific Inhibitory Mechanism of Aggregation of 42-mer Amyloid-βPeptide by Flavonoids (2012)
  • Author(s): Sato, M., Murakami, K., Ikubo, H., Uno, M., Nakagawa, Y., Katayama, S., Akagi, K., Masuda, Y., Takegoshi, K., Irie, K.
  • Organizer: IKCOC-12
  • Place of Presentation: Kyoto(ポスター発表, Poster Number PA-140)
  • Year and Date: 2012-11-13
• [Presentation] Cryocoil MASプローブによる固体有機物の^<33>S NMR測定にむけて (2012)
  • Author(s): 松尾真嗣,水野敬,野田泰斗,増田裕一,竹腰清乃理
  • Organizer: 第51回NMR討論会
  • Place of Presentation: 愛知(ポスター発表,講演番号P-81,査読なし)
  • Year and Date: 2012-11-10
• [Presentation] カテコール系フラボノイド類によるアミロイドβの凝集抑制機構 (2012)
  • Author(s): 村上一馬,佐藤瑞穂,井久保遥子,宇野真弓,中川優,片山寿美枝,赤木謙一,増田裕一,竹腰清乃理,入江一浩
  • Organizer: 第54回天然有機化合物討論会
  • Place of Presentation: 東京(口頭発表,講演番号17,査読あり)
  • Year and Date: 2012-09-19
• [Presentation] カテコール系フラボノイド類によるアミロイドβの凝集抑制機構 (2012)
  • Author(s): 村上一馬，佐藤瑞穂，井久保遥子，宇野真弓，中川 優，片山寿美枝，赤木謙一，増田裕一，竹腰清乃理，入江一浩
  • Organizer: 第54回天然有機化合物討論会
  • Place of Presentation: 東京農業大学 世田谷キャンパス
• [Presentation] Solid-State NMR Analysis of Interaction Sites of Curcumin and Amyloid beta-Protein Fibrils (2012)
  • Author(s): Yuichi Masuda, Masashi Fukuchi, Tatsuya Yatagawa, Masato Tada, Kazuyuki Takeda, Kazuhiro Irie, Ken-ichi Akagi, Youko Monobe, Takayoshi Imazawa, K. Takegoshi
  • Organizer: The 12th International Kyoto Conference on New Aspects of Organic Chemistry
  • Place of Presentation: Rihga Royal Hotel Kyoto
• [Presentation] Specific Inhibitory Mechanism of Aggregation of 42-mer Amyloid-beta Peptide by Flavonoids (2012)
  • Author(s): Mizuho Sato, Kazuma Murakami, Haruko Ikubo, Mayumi Uno, Yu Nakagawa, Sumie Katayama, Ken-ichi Akagi, Yuichi Masuda, Kiyonori Takegoshi, and Kazuhiro Irie
  • Organizer: The 12th International Kyoto Conference on New Aspects of Organic Chemistry
  • Place of Presentation: Rihga Royal Hotel Kyoto
• [Presentation] コラーゲン分泌阻害剤の合成研究
  • Author(s): 土井隆行，齋藤将純，伊藤進也，増田裕一，吉田将人，五島直樹，夏目徹，永田和宏
  • Organizer: 第51回日本薬学会東北支部大会
  • Place of Presentation: 青森大学
• [Presentation] アプラトキシンCの合成研究
  • Author(s): 土井隆行，鈴木淳，増田裕一，吉田将人
  • Organizer: 第51回日本薬学会東北支部大会
  • Place of Presentation: 青森大学
• [Book] 遺伝子医学MOOK第21巻「最新ペプチド合成技術とその創薬研究への応用」(木曽良明編) (2012)
  • Author(s): 村上一馬,増田裕一,入江一浩
• [Remarks]
  • URL: http://www.pharm.tohoku.ac.jp/~hannou/index.html
• [Remarks] 東北大学薬学部 大学院薬学研究科 反応制御化学分野のホームページ
  • URL: http://www.pharm.tohoku.ac.jp/~hannou/index.html
• [Remarks] 東北大学薬学部大学院薬学研究科 反応制御化学分野のホームページ Doi Laboratory
  • URL: http://www.pharm.tohoku.ac.jp/~hannou/index.html