Research Project
Grant-in-Aid for Young Scientists (B)
Cardiolipin (CL) is a unique phospholipid in mitochondrial membranes with diverse biological functions. Induction of the peroxidase activity of cyt c by CL binding in the presence of hydrogen peroxide has been thought to be a key event in early apoptosis stage.We synthesized a series of oxidized CLs in which four acyl chains were oxidized in different patterns. All oxidized CLs elicited strong inducing ability of the peroxidase activity of cyt c. A difference in the oxidized position including the number of OH group did not affect the inducing ability. Our results do not support a currently accepted notion that hydrophobic interaction between the acyl chains of CL and cyt c is critical for the formation of specific CL-cyt c complex and consequent induction of the peroxide activity.Moreover, we synthesized some photoreactive CLs. By photoaffinity labeling experiments, the labeled peptide was detected by attaching a biotin to the incorporated ethynyl group by click chemistry.
All 2014 2013 2012 Other
All Journal Article (8 results) (of which Peer Reviewed: 8 results, Open Access: 1 results) Presentation (15 results) Remarks (3 results)
Phytochemistry
Volume: 96 Pages: 223-234
10.1016/j.phytochem.2013.10.001
FEBS Open Bio.
Volume: 3 Issue: 1 Pages: 406-410
10.1016/j.fob.2013.09.007
Bioorganic & Medicinal Chemistry Letters
Volume: 23 Issue: 5 Pages: 1217-1219
10.1016/j.bmcl.2013.01.018
Volume: 96 Pages: 132-147
10.1016/j.phytochem.2013.08.013
Chem. Res. Toxic.
Volume: 25 Pages: 2253-2260
Volume: 84 Pages: 56-67
Chem. Res. Toxicol
Volume: 25 Issue: 10 Pages: 2253-2260
10.1021/tx300315h
10.1016/j.phytochem.2012.08.001
http://www.biofunc-chem.kais.kyoto-u.ac.jp/
