| Project/Area Number |
24780115
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Bioproduction chemistry/Bioorganic chemistry
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| Research Institution | Osaka Prefecture University |
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Principal Investigator |
KAI Kenji 大阪府立大学, 生命環境科学研究科(系), 助教 (40508404)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
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| Keywords | エンドバクテリア / 接合菌 / Mortierella alpina / クオラムセンシング / 共生 |
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| Research Abstract |
Increasing evidence indicates that certain bacteria, namely endobacteria, inhabit fungal cells and establish symbiotic relationships with hosts. However, it has not been clear whether bacterial QS acts in developing the relationships. We isolated and identified C7-HSL and C8-HSL from the culture broth of Mortierella alpina A-178. This suggested the presence of endobacteria in the fungus, which was confirmed by PCR, FISH, and TEM. Two major bands detected by PCR-denaturing gradient gel electrophoresis showed sequence identity with Castellaniella defragrans and Cryobacterium sp. The production ofAHLs depended on the presence of endobacteria and was induced in response to the increase in the concentration of AHLs, suggesting that the bacterium conducts AHL-mediated QS in the fungus. This research is the first to report the production of AHLs by endofungal bacteria and raises the possibility that QS plays roles in the development of fungus-endobacterium symbiosis.
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