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Identification of a novel plant hormone-like compound that regulates light sensitivity of plant

Research Project

Project/Area Number 24780117
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Bioproduction chemistry/Bioorganic chemistry
Research InstitutionTohoku University

Principal Investigator

SETO YOSHIYA  東北大学, 生命科学研究科, 助教 (40620282)

Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
KeywordsD14LIKE / 胚軸伸長 / カリキン / SMAX1 / 立体特異性 / ストリゴラクトン / 光感受性
Outline of Final Research Achievements

In this research work, I focused on D14LIKE protein which is an alpha/beta-fold-hydrolase protein involved in hypocotyl elongation and seed germination in Arabidopsis. We tried to identify the endogenous ligand for this protein, and established an yeast two hybrid system using D14LIKE and its possible partner protein, SMAX1. This system can be used as a bioassay method to find the D14LIKE ligand.
Strigolactone (SL) is a plant hormone that regulates shoot branching, and D14 protein, a closely related homolog of D14LIKE, acts as a possible receptor for SL. SL was also reported to inhibit hypocotyl elongation of Arabidopsis, thus I carried out structure activity relationship study using optically purified stereoisomers of SL. As a result we found that there is no stereo-specificity for the D14LIKE-dependent effect of SL, suggesting that SL itself is not the endogenous ligand for the D14LIKE pathway.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (18 results)

All 2015 2014 2013 2012

All Journal Article (7 results) (of which Peer Reviewed: 6 results,  Acknowledgement Compliant: 2 results) Presentation (10 results) (of which Invited: 1 results) Book (1 results)

  • [Journal Article] Structural requirements of strigolactones for shoot branching inhibition in rice and Arabidopsis2015

    • Author(s)
      Mikihisa Umehara, Mengmeng Cao, Kohki Akiyama, Tomoki Akatsu, Yoshiya Seto, Atsushi Hanada, Li Weiqiang, Noriko Takeda-Kamiya, Yu Morimoto, Shinjiro Yamaguchi
    • Journal Title

      Plant and cell physiology

      Volume: 印刷中 Issue: 6 Pages: 1059-1072

    • DOI

      10.1093/pcp/pcv028

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Strigolactone biosynthesis and perception2014

    • Author(s)
      Seto, Y., Yamaguchi, S.
    • Journal Title

      Curr Opin Plant Biol

      Volume: 21 Pages: 1-6

    • DOI

      10.1016/j.pbi.2014.06.001

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Carlactone is converted to carlactonoic acid by MAX1 in Arabidopsis and its methyl ester can directly interact with AtD14 in vitro2014

    • Author(s)
      Abe, S., Sado, A., Tanaka, K., Kisugi, T., Asami, K., Ota, S., Kim, H.I., Yoneyama, K., Xie, X., Ohnishi, T., Seto, Y., Yamaguchi, S., Akiyama, K., Yoneyama, K., Nomura, T.
    • Journal Title

      Proc Natl Acad Sci USA

      Volume: 111 Issue: 50 Pages: 18084-18089

    • DOI

      10.1073/pnas.1410801111

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Carlactone is an endogenous biosynthetic precursor for strigolactones2014

    • Author(s)
      Yoshiya Seto, Aika Sado, Kei Asami, Atsushi Hanada, Mikihisa Umehara, Kohki Akiyama, Shinjiro Yamaguchi
    • Journal Title

      Proc Natl Sci USA

      Volume: 111 Pages: 1640-1645

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] ストリゴラクトンの多様な生理作用と生合成2013

    • Author(s)
      山田雄介、梅原三貴久、瀬戸義哉
    • Journal Title

      植物の生長調節

      Volume: 48 Pages: 148-153

    • NAID

      110009687191

    • Related Report
      2013 Research-status Report
  • [Journal Article] Structures of D14 and D14L in the strigolactone and karrikin signaling pathways2013

    • Author(s)
      Kagiyama M, Hirano Y, Mori T, Kim SY, Kyozuka J, Seto Y, Yamaguchi S, Hakoshima T
    • Journal Title

      Genes Cells.

      Volume: 18 Issue: 2 Pages: 147-160

    • DOI

      10.1111/gtc.12025

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Recent advances in strigolactone research: chemical and biological aspects.2012

    • Author(s)
      Yoshiya Seto
    • Journal Title

      Plant Cell Physiology

      Volume: 53 Issue: 11 Pages: 1843-1853

    • DOI

      10.1093/pcp/pcs142

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] ケミカルスクリーニングによるD14を標的とする新規ストリゴラクトンアゴニストの探索2015

    • Author(s)
      安井令、瀬戸義哉、笠原博幸、山口信次郎
    • Organizer
      第56回日本植物生理学会年会
    • Place of Presentation
      東京農業大学
    • Year and Date
      2015-03-16 – 2015-03-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] シロイヌナズナにおけるカーラクトン酸メチルの同定、及びその枝分かれ抑制活性に関する研究2015

    • Author(s)
      瀬戸義哉、浅見慶、太田彩恵子、田中海、佐渡愛香、秋山康紀、阿部聡子、野村崇人、山口信次郎
    • Organizer
      第56回日本植物生理学会年会
    • Place of Presentation
      東京農業大学
    • Year and Date
      2015-03-16 – 2015-03-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] Chemical screening of novel strigolactone agonists that target D14 protein2015

    • Author(s)
      Yoshiya Seto, Rei Yasui, Hiroyuki Kasahara, Shinjiro Yamaguchi
    • Organizer
      1st international congress on strigolactones
    • Place of Presentation
      Wageningen, Netherlands
    • Year and Date
      2015-03-01 – 2015-03-06
    • Related Report
      2014 Annual Research Report
  • [Presentation] シロイヌナズナにおけるカーラクトンの動態2014

    • Author(s)
      浅見慶、浅見慶、佐渡愛香、来生貴也、花田篤志、瀬戸義哉、秋山康紀、山口信次郎
    • Organizer
      植物化学調節学会 第49回大会
    • Place of Presentation
      京都大学
    • Year and Date
      2014-10-17 – 2014-10-19
    • Related Report
      2014 Annual Research Report
  • [Presentation] ストリゴラクトン生合成中間体carlactonoic acidの同定2014

    • Author(s)
      田中海、佐渡愛香、浅見慶、瀬戸義哉、秋山康紀、山口信次郎
    • Organizer
      植物化学調節学会 第49回大会
    • Place of Presentation
      京都大学
    • Year and Date
      2014-10-17 – 2014-10-19
    • Related Report
      2014 Annual Research Report
  • [Presentation] ストリゴラクトンの生合成と動態2014

    • Author(s)
      瀬戸義哉
    • Organizer
      日本育種学会第125回講演会
    • Place of Presentation
      仙台
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] 安定同位体標識カーラクトンを用いたシロイヌナズナにおけるストリゴラクトンの生合成研究2014

    • Author(s)
      浅見慶、瀬戸義哉、花田篤志、佐渡愛香、秋山康紀、山口信次郎
    • Organizer
      第55回日本植物生理学会
    • Place of Presentation
      富山
    • Related Report
      2013 Research-status Report
  • [Presentation] シロイヌナズナのロゼット葉におけるストリゴラクトンの生理作用2013

    • Author(s)
      森本優、東大野むつみ、瀬戸義哉、桧垣匠、馳澤盛一郎、山口信次郎
    • Organizer
      植物化学調節学会第48回大会
    • Place of Presentation
      新潟
    • Related Report
      2013 Research-status Report
  • [Presentation] D14相互作用因子の探索2012

    • Author(s)
      Seo Eunjoo,、瀬戸 義哉、瀬尾 光範、山口 信次郎
    • Organizer
      植物化学調節学会
    • Place of Presentation
      山形
    • Related Report
      2012 Research-status Report
  • [Presentation] シロイヌナズナのロゼット葉におけるストリゴラクトンの生理作用2012

    • Author(s)
      森本 優、瀬戸 義哉、山口 信次郎
    • Organizer
      植物化学調節学会
    • Place of Presentation
      山形
    • Related Report
      2012 Research-status Report
  • [Book] バイオサイエンスとインダストリー2014

    • Author(s)
      瀬戸義哉、秋山康紀、山口信次郎
    • Total Pages
      2
    • Publisher
      バイオインダストリー協会
    • Related Report
      2014 Annual Research Report

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Published: 2013-05-31   Modified: 2019-07-29  

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