Research Project
Grant-in-Aid for Young Scientists (B)
In this research work, I focused on D14LIKE protein which is an alpha/beta-fold-hydrolase protein involved in hypocotyl elongation and seed germination in Arabidopsis. We tried to identify the endogenous ligand for this protein, and established an yeast two hybrid system using D14LIKE and its possible partner protein, SMAX1. This system can be used as a bioassay method to find the D14LIKE ligand.Strigolactone (SL) is a plant hormone that regulates shoot branching, and D14 protein, a closely related homolog of D14LIKE, acts as a possible receptor for SL. SL was also reported to inhibit hypocotyl elongation of Arabidopsis, thus I carried out structure activity relationship study using optically purified stereoisomers of SL. As a result we found that there is no stereo-specificity for the D14LIKE-dependent effect of SL, suggesting that SL itself is not the endogenous ligand for the D14LIKE pathway.
All 2015 2014 2013 2012
All Journal Article (7 results) (of which Peer Reviewed: 6 results, Acknowledgement Compliant: 2 results) Presentation (10 results) (of which Invited: 1 results) Book (1 results)
Plant and cell physiology
Volume: 印刷中 Issue: 6 Pages: 1059-1072
10.1093/pcp/pcv028
Curr Opin Plant Biol
Volume: 21 Pages: 1-6
10.1016/j.pbi.2014.06.001
Proc Natl Acad Sci USA
Volume: 111 Issue: 50 Pages: 18084-18089
10.1073/pnas.1410801111
Proc Natl Sci USA
Volume: 111 Pages: 1640-1645
植物の生長調節
Volume: 48 Pages: 148-153
110009687191
Genes Cells.
Volume: 18 Issue: 2 Pages: 147-160
10.1111/gtc.12025
Plant Cell Physiology
Volume: 53 Issue: 11 Pages: 1843-1853
10.1093/pcp/pcs142