| Project/Area Number |
24780122
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Food science
|
|---|
| Research Institution | Niigata University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2012-04-01 – 2014-03-31
|
| Project Status |
Completed (Fiscal Year 2013)
|
| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
|
|---|
| Keywords | 歯周病菌 / 増殖阻害 / α-アミラーゼ / Hsp70 / Ｘ線結晶構造解析 / イネタンパク質 / 抗菌性タンパク質 / 抗菌タンパク質 |
|---|
| Research Abstract |
In a recent study, we identified two proteins from rice (Oryza sativa) seed, OsHsp70N (Hsp70 homolog) and AmyI-1 (alpha-amylase),that show significant cell growth-inhibitory activity against periodontal pathogenic bacteria. In this study, we investigated the spectrum of activity of these proteins against the bacteria, and the crystal structures of the proteins were also determined. Crystallographic and intermolecular interaction analysis showed that AmyI-1 interacts with lipopolysaccharide, a component of the cell wall of gram-negative bacteria, and suggested that AmyI-1 and starch hydrolysate have a synergistic effect on the inhibition of cellular growth in these bacteria.
|
