Analysis of the coordinative function between a milk antimicrobial protein and an intestinal epithelial lectin for microbial colonization in the infant intestine

Research Project

Project/Area Number	24780126
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Food science
Research Institution	Nagoya University
Principal Investigator	OSHIMA Kenzi 名古屋大学, 生命農学研究科, 助教 (90391888)
Project Period (FY)	2012-04-01 – 2014-03-31
Project Status	Completed (Fiscal Year 2013)
Budget Amount *help	¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000) Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000) Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords	腸内細菌 / ラクトフェリン / 食品機能
Research Abstract	We suggest a hypothetical model in which anti-bacterial protein lactoferrin (LF), which is ingested as a component of milk, and intestinal protein, intelectin, bind and cooperate to regulate primary intestinal flora development in the infant digestive tract. This study validated this hypothesis and obtained following results. 1) Intelectin partially localized in some specific cellular compartments and affected LF intake by the cultured intestinal epithelial cells. 2) Intelectin and LF differently promoted growth of some Bifidobacterium lines. 3) To analyze interaction between intestinal epithelia and microbes in the culture system, several cell lines with the various characters were isolated from heterogenous HT29 cell line.