| Project/Area Number |
24780128
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Food science
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| Research Institution | Kobe University |
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Principal Investigator |
AIHARA Yoshiko 神戸大学, (連合)農学研究科(研究院), 助教 (30620877)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Keywords | 消化管ホルモン / ファイトケミカル / フラボノイド |
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| Research Abstract |
We constructed a novel bioassay system to evaluate ligands of gut hormone secretion using cultured cells (Caco-2). Caco-2 was found to release glucagon-like peptide 1 (GLP-1), peptide YY (PYY), and cholecystokinin (CCK) by adding sugars, free fatty acids, and amino acids, respectively. A screening among phytochemicals using Caco-2 cells showed that (-)-epigallocatechin gallate (EGCG) and ferulic acid can stimulate release of gut hormones. By using dissected gut rings of mice, it was demonstrated that EGCG induce gut hormone release of GLP-1, PYY and CCK from animal tissues. These results indicate that Caco-2 is useful tool to evaluate gut hormone secretion in vitro. According to a gene expression analysis of stimulated Caco-2 cells by EGCG, it is shown that a membrane receptor is involved in a signaling cascade for exocytosis of hormone.
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